ID   ECPA_ECOLI              Reviewed;         195 AA.
AC   P0AAA3; P77264; Q2MCC9;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Common pilus major fimbrillin subunit EcpA;
DE   AltName: Full=MatB fimbrillin;
DE   Flags: Precursor;
GN   Name=ecpA; Synonyms=matB, yagZ; OrderedLocusNames=b0293, JW0287;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RA   Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA   Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA   Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT   "Sequence of minutes 4-25 of Escherichia coli.";
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   LACK OF EXPRESSION.
RC   STRAIN=K12;
RX   PubMed=11466275; DOI=10.1128/jb.183.16.4727-4736.2001;
RA   Pouttu R., Westerlund-Wikstrom B., Lang H., Alsti K., Virkola R.,
RA   Saarela U., Siitonen A., Kalkkinen N., Korhonen T.K.;
RT   "matB, a common fimbrillin gene of Escherichia coli, expressed in a
RT   genetically conserved, virulent clonal group.";
RL   J. Bacteriol. 183:4727-4736(2001).
RN   [5]
RP   EXPRESSION.
RC   STRAIN=K12;
RX   PubMed=20522494; DOI=10.1099/mic.0.039610-0;
RA   Lehti T.A., Bauchart P., Heikkinen J., Hacker J., Korhonen T.K.,
RA   Dobrindt U., Westerlund-Wikstrom B.;
RT   "Mat fimbriae promote biofilm formation by meningitis-associated
RT   Escherichia coli.";
RL   Microbiology 156:2408-2417(2010).
CC   -!- FUNCTION: Part of the ecpRABCDE operon, which encodes the E.coli common
CC       pilus (ECP). ECP is found in both commensal and pathogenic strains and
CC       plays a dual role in early-stage biofilm development and host cell
CC       recognition. Major subunit of the fimbria (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Self-associates. Forms filaments. Interacts with EcpD (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Fimbrium {ECO:0000250}.
CC   -!- INDUCTION: Negatively regulated by H-NS. Positively regulated by IHF
CC       and EcpR (By similarity). {ECO:0000250}.
CC   -!- MISCELLANEOUS: Not expressed under classical laboratory conditions, but
CC       is functional when constitutively expressed (PubMed:11466275 and
CC       PubMed:20522494).
CC   -!- SIMILARITY: Belongs to the EcpA/MatB fimbrillin family. {ECO:0000305}.
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DR   EMBL; U73857; AAB18022.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC73396.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE76077.1; -; Genomic_DNA.
DR   PIR; E64755; E64755.
DR   RefSeq; NP_414827.1; NC_000913.3.
DR   RefSeq; WP_000730972.1; NZ_SSZK01000048.1.
DR   AlphaFoldDB; P0AAA3; -.
DR   SMR; P0AAA3; -.
DR   BioGRID; 4259792; 22.
DR   STRING; 511145.b0293; -.
DR   PaxDb; P0AAA3; -.
DR   EnsemblBacteria; AAC73396; AAC73396; b0293.
DR   EnsemblBacteria; BAE76077; BAE76077; BAE76077.
DR   GeneID; 948759; -.
DR   KEGG; ecj:JW0287; -.
DR   KEGG; eco:b0293; -.
DR   PATRIC; fig|1411691.4.peg.1985; -.
DR   EchoBASE; EB3335; -.
DR   eggNOG; ENOG502Z9JQ; Bacteria.
DR   HOGENOM; CLU_120328_0_0_6; -.
DR   OMA; AQDQFTF; -.
DR   BioCyc; EcoCyc:G6164-MON; -.
DR   PRO; PR:P0AAA3; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0009289; C:pilus; IEA:UniProtKB-SubCell.
DR   Gene3D; 2.60.40.3290; -; 1.
DR   InterPro; IPR016514; EcpA.
DR   InterPro; IPR038478; Fimbrillin_EcpA_sf.
DR   Pfam; PF16449; MatB; 1.
DR   PIRSF; PIRSF007320; Fimbrillin_MatB; 1.
PE   3: Inferred from homology;
KW   Fimbrium; Reference proteome; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..195
FT                   /note="Common pilus major fimbrillin subunit EcpA"
FT                   /id="PRO_0000013790"
SQ   SEQUENCE   195 AA;  20111 MW;  60791615380A2A44 CRC64;
     MKKKVLAIAL VTVFTGMGVA QAADVTAQAV ATWSATAKKD TTSKLVVTPL GSLAFQYAEG
     IKGFNSQKGL FDVAIEGDST ATAFKLTSRL ITNTLTQLDT SGSTLNVGVD YNGAAVEKTG
     DTVMIDTANG VLGGNLSPLA NGYNASNRTT AQDGFTFSII SGTTNGTTAV TDYSTLPEGI
     WSGDVSVQFD ATWTS