ECPB_ECOLI
ID ECPB_ECOLI Reviewed; 222 AA.
AC P77188; Q2MCD0;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Probable fimbrial chaperone EcpB;
DE Flags: Precursor;
GN Name=ecpB; Synonyms=matC, yagY; OrderedLocusNames=b0292, JW0286;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP LACK OF EXPRESSION.
RC STRAIN=K12;
RX PubMed=11466275; DOI=10.1128/jb.183.16.4727-4736.2001;
RA Pouttu R., Westerlund-Wikstrom B., Lang H., Alsti K., Virkola R.,
RA Saarela U., Siitonen A., Kalkkinen N., Korhonen T.K.;
RT "matB, a common fimbrillin gene of Escherichia coli, expressed in a
RT genetically conserved, virulent clonal group.";
RL J. Bacteriol. 183:4727-4736(2001).
RN [5]
RP EXPRESSION.
RC STRAIN=K12;
RX PubMed=20522494; DOI=10.1099/mic.0.039610-0;
RA Lehti T.A., Bauchart P., Heikkinen J., Hacker J., Korhonen T.K.,
RA Dobrindt U., Westerlund-Wikstrom B.;
RT "Mat fimbriae promote biofilm formation by meningitis-associated
RT Escherichia coli.";
RL Microbiology 156:2408-2417(2010).
CC -!- FUNCTION: Part of the ecpRABCDE operon, which encodes the E.coli common
CC pilus (ECP). ECP is found in both commensal and pathogenic strains and
CC plays a dual role in early-stage biofilm development and host cell
CC recognition (By similarity). {ECO:0000250}.
CC -!- INTERACTION:
CC P77188; P62620: ispG; NbExp=2; IntAct=EBI-1113651, EBI-550338;
CC -!- INDUCTION: Negatively regulated by H-NS. Positively regulated by IHF
CC and EcpR (By similarity). {ECO:0000250}.
CC -!- MISCELLANEOUS: Not expressed under classical laboratory conditions, but
CC is functional when constitutively expressed (PubMed:11466275 and
CC PubMed:20522494).
CC -!- SIMILARITY: Belongs to the EcpB/EcpE family. {ECO:0000305}.
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DR EMBL; U73857; AAB18021.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73395.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76076.1; -; Genomic_DNA.
DR PIR; D64755; D64755.
DR RefSeq; NP_414826.1; NC_000913.3.
DR RefSeq; WP_000716398.1; NZ_STEB01000020.1.
DR PDB; 5DFK; X-ray; 2.40 A; A=21-222.
DR PDBsum; 5DFK; -.
DR AlphaFoldDB; P77188; -.
DR SMR; P77188; -.
DR BioGRID; 4259791; 5.
DR BioGRID; 853088; 1.
DR IntAct; P77188; 53.
DR STRING; 511145.b0292; -.
DR PaxDb; P77188; -.
DR PRIDE; P77188; -.
DR EnsemblBacteria; AAC73395; AAC73395; b0292.
DR EnsemblBacteria; BAE76076; BAE76076; BAE76076.
DR GeneID; 66671413; -.
DR GeneID; 948806; -.
DR KEGG; ecj:JW0286; -.
DR KEGG; eco:b0292; -.
DR PATRIC; fig|511145.12.peg.296; -.
DR EchoBASE; EB3334; -.
DR eggNOG; COG3121; Bacteria.
DR HOGENOM; CLU_106652_0_0_6; -.
DR OMA; KENYFVM; -.
DR BioCyc; EcoCyc:G6163-MON; -.
DR PRO; PR:P77188; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR040695; EcpB_C.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR008962; PapD-like_sf.
DR Pfam; PF18649; EcpB_C; 1.
DR SUPFAM; SSF49354; SSF49354; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chaperone; Fimbrium biogenesis; Reference proteome; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..222
FT /note="Probable fimbrial chaperone EcpB"
FT /id="PRO_0000013789"
FT STRAND 26..30
FT /evidence="ECO:0007829|PDB:5DFK"
FT STRAND 35..43
FT /evidence="ECO:0007829|PDB:5DFK"
FT STRAND 45..47
FT /evidence="ECO:0007829|PDB:5DFK"
FT STRAND 49..59
FT /evidence="ECO:0007829|PDB:5DFK"
FT STRAND 75..79
FT /evidence="ECO:0007829|PDB:5DFK"
FT STRAND 81..85
FT /evidence="ECO:0007829|PDB:5DFK"
FT STRAND 89..97
FT /evidence="ECO:0007829|PDB:5DFK"
FT STRAND 106..113
FT /evidence="ECO:0007829|PDB:5DFK"
FT STRAND 136..143
FT /evidence="ECO:0007829|PDB:5DFK"
FT STRAND 152..155
FT /evidence="ECO:0007829|PDB:5DFK"
FT STRAND 158..161
FT /evidence="ECO:0007829|PDB:5DFK"
FT STRAND 163..165
FT /evidence="ECO:0007829|PDB:5DFK"
FT STRAND 167..177
FT /evidence="ECO:0007829|PDB:5DFK"
FT HELIX 178..180
FT /evidence="ECO:0007829|PDB:5DFK"
FT STRAND 184..190
FT /evidence="ECO:0007829|PDB:5DFK"
FT STRAND 195..197
FT /evidence="ECO:0007829|PDB:5DFK"
FT STRAND 199..201
FT /evidence="ECO:0007829|PDB:5DFK"
FT STRAND 209..214
FT /evidence="ECO:0007829|PDB:5DFK"
FT STRAND 217..222
FT /evidence="ECO:0007829|PDB:5DFK"
SQ SEQUENCE 222 AA; 24517 MW; 2B47AE6230FCC8D1 CRC64;
MKKHLLPLAL LFSGISPAQA LDVGDISSFM NSDSSTLSKT IKNSTDSGRL INIRLERLSS
PLDDGQVISM DKPDELLLTP ASLLLPAQAS EVIRFFYKGP ADEKERYYRI VWFDQALSDA
QRDNANRSAV ATASARIGTI LVVAPRQANY HFQYANGSLT NTGNATLRIL AYGPCLKAAN
GKECKENYYL MPGKSRRFTR VDTADNKGRV ALWQGDKFIP VK
