ID   ECPR_ECO27              Reviewed;         196 AA.
AC   B7UJE2;
DT   09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=HTH-type transcriptional regulator EcpR;
GN   Name=ecpR; OrderedLocusNames=E2348C_0250;
OS   Escherichia coli O127:H6 (strain E2348/69 / EPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=574521;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=E2348/69 / EPEC;
RX   PubMed=18952797; DOI=10.1128/jb.01238-08;
RA   Iguchi A., Thomson N.R., Ogura Y., Saunders D., Ooka T., Henderson I.R.,
RA   Harris D., Asadulghani M., Kurokawa K., Dean P., Kenny B., Quail M.A.,
RA   Thurston S., Dougan G., Hayashi T., Parkhill J., Frankel G.;
RT   "Complete genome sequence and comparative genome analysis of
RT   enteropathogenic Escherichia coli O127:H6 strain E2348/69.";
RL   J. Bacteriol. 191:347-354(2009).
RN   [2]
RP   FUNCTION, DNA-BINDING, INDUCTION, AND MUTAGENESIS OF ASP-60; GLY-159;
RP   THR-175; VAL-176 AND LYS-186.
RC   STRAIN=E2348/69 / EPEC;
RX   PubMed=22797761; DOI=10.1128/jb.00915-12;
RA   Martinez-Santos V.I., Medrano-Lopez A., Saldana Z., Giron J.A.,
RA   Puente J.L.;
RT   "Transcriptional regulation of the ecp operon by EcpR, IHF, and H-NS in
RT   attaching and effacing Escherichia coli.";
RL   J. Bacteriol. 194:5020-5033(2012).
CC   -!- FUNCTION: Part of the ecpRABCDE operon, which encodes the E.coli common
CC       pilus (ECP). ECP is found in both commensal and pathogenic strains and
CC       plays a dual role in early-stage biofilm development and host cell
CC       recognition (By similarity). Positively regulates the expression of the
CC       ecp operon by binding to two TTCCT boxes. {ECO:0000250,
CC       ECO:0000269|PubMed:22797761}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- INDUCTION: Negatively regulated by H-NS. Positively autoregulated. Also
CC       positively regulated by IHF. {ECO:0000269|PubMed:22797761}.
CC   -!- SIMILARITY: Belongs to the EcpR/MatA family. {ECO:0000305}.
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DR   EMBL; FM180568; CAS07798.1; -; Genomic_DNA.
DR   AlphaFoldDB; B7UJE2; -.
DR   SMR; B7UJE2; -.
DR   EnsemblBacteria; CAS07798; CAS07798; E2348C_0250.
DR   KEGG; ecg:E2348C_0250; -.
DR   HOGENOM; CLU_128111_0_0_6; -.
DR   OMA; CIWPAHD; -.
DR   Proteomes; UP000008205; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:2000144; P:positive regulation of DNA-templated transcription, initiation; IMP:CACAO.
DR   CDD; cd06170; LuxR_C_like; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF00196; GerE; 1.
DR   PRINTS; PR00038; HTHLUXR.
DR   SMART; SM00421; HTH_LUXR; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
DR   PROSITE; PS50043; HTH_LUXR_2; 1.
PE   1: Evidence at protein level;
KW   Activator; Cytoplasm; DNA-binding; Transcription; Transcription regulation.
FT   CHAIN           1..196
FT                   /note="HTH-type transcriptional regulator EcpR"
FT                   /id="PRO_0000429480"
FT   DOMAIN          138..196
FT                   /note="HTH luxR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT   DNA_BIND        162..181
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT   MUTAGEN         60
FT                   /note="D->A: Decrease in activity."
FT                   /evidence="ECO:0000269|PubMed:22797761"
FT   MUTAGEN         159
FT                   /note="G->A: Decrease in activity."
FT                   /evidence="ECO:0000269|PubMed:22797761"
FT   MUTAGEN         175
FT                   /note="T->A: Decrease in activity."
FT                   /evidence="ECO:0000269|PubMed:22797761"
FT   MUTAGEN         176
FT                   /note="V->A: Lack of activity."
FT                   /evidence="ECO:0000269|PubMed:22797761"
FT   MUTAGEN         186
FT                   /note="K->A: Lack of activity."
FT                   /evidence="ECO:0000269|PubMed:22797761"
SQ   SEQUENCE   196 AA;  23299 MW;  424D68D2B471154B CRC64;
     MTWQNDYSRD YEVKNHMECQ NRSDKYIWSP HDAYFYKGLS ELIVDIDRLI YLSLEKIRKD
     FVFINLNTDS LTEFINRDNE WLSAVKGKQV VLIAARKSEA LANYWYYNSN IRGVVYAGLS
     RDIRKELAYV INGRFLRKDI KKDKITDREM EIIRMTAQGM LPKSIARIEN CSVKTVYTHR
     RNAEAKLYSK LYKLVQ