ID   ECPR_ECOKI              Reviewed;         196 AA.
AC   Q9AME2; D5CVK1;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=HTH-type transcriptional regulator EcpR;
DE   AltName: Full=Meningitis associated and temperature regulated protein A;
GN   Name=ecpR; Synonyms=matA; OrderedLocusNames=ECOK1_0281;
OS   Escherichia coli O18:K1:H7 (strain IHE3034 / ExPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=714962;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=IHE3034 / ExPEC;
RX   PubMed=11466275; DOI=10.1128/jb.183.16.4727-4736.2001;
RA   Pouttu R., Westerlund-Wikstrom B., Lang H., Alsti K., Virkola R.,
RA   Saarela U., Siitonen A., Kalkkinen N., Korhonen T.K.;
RT   "matB, a common fimbrillin gene of Escherichia coli, expressed in a
RT   genetically conserved, virulent clonal group.";
RL   J. Bacteriol. 183:4727-4736(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IHE3034 / ExPEC;
RX   PubMed=20439758; DOI=10.1073/pnas.0915077107;
RA   Moriel D.G., Bertoldi I., Spagnuolo A., Marchi S., Rosini R., Nesta B.,
RA   Pastorello I., Corea V.A., Torricelli G., Cartocci E., Savino S.,
RA   Scarselli M., Dobrindt U., Hacker J., Tettelin H., Tallon L.J.,
RA   Sullivan S., Wieler L.H., Ewers C., Pickard D., Dougan G., Fontana M.R.,
RA   Rappuoli R., Pizza M., Serino L.;
RT   "Identification of protective and broadly conserved vaccine antigens from
RT   the genome of extraintestinal pathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 107:9072-9077(2010).
RN   [3]
RP   FUNCTION.
RC   STRAIN=IHE3034 / ExPEC;
RX   PubMed=20522494; DOI=10.1099/mic.0.039610-0;
RA   Lehti T.A., Bauchart P., Heikkinen J., Hacker J., Korhonen T.K.,
RA   Dobrindt U., Westerlund-Wikstrom B.;
RT   "Mat fimbriae promote biofilm formation by meningitis-associated
RT   Escherichia coli.";
RL   Microbiology 156:2408-2417(2010).
RN   [4]
RP   FUNCTION IN REPRESSION OF FLHDC, AND DNA-BINDING.
RC   STRAIN=IHE3034 / ExPEC;
RX   PubMed=22422754; DOI=10.1099/mic.0.056499-0;
RA   Lehti T.A., Bauchart P., Dobrindt U., Korhonen T.K.,
RA   Westerlund-Wikstrom B.;
RT   "The fimbriae activator MatA switches off motility in Escherichia coli by
RT   repression of the flagellar master operon flhDC.";
RL   Microbiology 158:1444-1455(2012).
CC   -!- FUNCTION: Part of the ecpRABCDE operon, which encodes the E.coli common
CC       pilus (ECP). ECP is found in both commensal and pathogenic strains and
CC       plays a dual role in early-stage biofilm development and host cell
CC       recognition. Positively regulates the expression of the ecp operon.
CC       Also represses expression of the flagellar master operon flhDC, and
CC       consequently prevents flagellum biosynthesis and motility. Acts by
CC       binding to the regulatory region of the flhDC operon.
CC       {ECO:0000269|PubMed:11466275, ECO:0000269|PubMed:20522494,
CC       ECO:0000269|PubMed:22422754}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- INDUCTION: Negatively regulated by H-NS. Positively autoregulated. Also
CC       positively regulated by IHF (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the EcpR/MatA family. {ECO:0000305}.
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DR   EMBL; AF325731; AAK01670.1; -; Genomic_DNA.
DR   EMBL; CP001969; ADE91099.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9AME2; -.
DR   SMR; Q9AME2; -.
DR   KEGG; eih:ECOK1_0281; -.
DR   PATRIC; fig|714962.3.peg.281; -.
DR   HOGENOM; CLU_128111_0_0_6; -.
DR   OMA; CIWPAHD; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd06170; LuxR_C_like; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF00196; GerE; 1.
DR   PRINTS; PR00038; HTHLUXR.
DR   SMART; SM00421; HTH_LUXR; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
DR   PROSITE; PS50043; HTH_LUXR_2; 1.
PE   1: Evidence at protein level;
KW   Activator; Cytoplasm; DNA-binding; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..196
FT                   /note="HTH-type transcriptional regulator EcpR"
FT                   /id="PRO_0000369171"
FT   DOMAIN          138..196
FT                   /note="HTH luxR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT   DNA_BIND        162..181
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
SQ   SEQUENCE   196 AA;  23299 MW;  424D68D2B471154B CRC64;
     MTWQNDYSRD YEVKNHMECQ NRSDKYIWSP HDAYFYKGLS ELIVDIDRLI YLSLEKIRKD
     FVFINLNTDS LTEFINRDNE WLSAVKGKQV VLIAARKSEA LANYWYYNSN IRGVVYAGLS
     RDIRKELAYV INGRFLRKDI KKDKITDREM EIIRMTAQGM LPKSIARIEN CSVKTVYTHR
     RNAEAKLYSK LYKLVQ