ECPR_ECOLI
ID ECPR_ECOLI Reviewed; 196 AA.
AC P71301; Q2MCC8;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=HTH-type transcriptional regulator EcpR;
GN Name=ecpR; Synonyms=matA, ykgK; OrderedLocusNames=b0294, JW5031;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP EXPRESSION.
RC STRAIN=K12;
RX PubMed=11466275; DOI=10.1128/jb.183.16.4727-4736.2001;
RA Pouttu R., Westerlund-Wikstrom B., Lang H., Alsti K., Virkola R.,
RA Saarela U., Siitonen A., Kalkkinen N., Korhonen T.K.;
RT "matB, a common fimbrillin gene of Escherichia coli, expressed in a
RT genetically conserved, virulent clonal group.";
RL J. Bacteriol. 183:4727-4736(2001).
RN [5]
RP FUNCTION, AND MUTAGENESIS OF HIS-179.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=22422754; DOI=10.1099/mic.0.056499-0;
RA Lehti T.A., Bauchart P., Dobrindt U., Korhonen T.K.,
RA Westerlund-Wikstrom B.;
RT "The fimbriae activator MatA switches off motility in Escherichia coli by
RT repression of the flagellar master operon flhDC.";
RL Microbiology 158:1444-1455(2012).
CC -!- FUNCTION: Part of the ecpRABCDE operon, which encodes the E.coli common
CC pilus (ECP). ECP is found in both commensal and pathogenic strains and
CC plays a dual role in early-stage biofilm development and host cell
CC recognition. Positively regulates the expression of the ecp operon (By
CC similarity). Also represses expression of the flagellar master operon
CC flhDC, and consequently prevents flagellum biosynthesis and motility.
CC Acts by binding to the regulatory region of the flhDC operon
CC (Probable). {ECO:0000250, ECO:0000269|PubMed:22422754, ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- INDUCTION: Negatively regulated by H-NS. Positively autoregulated. Also
CC positively regulated by IHF (By similarity). {ECO:0000250}.
CC -!- MISCELLANEOUS: Expressed in strain K12. {ECO:0000305|PubMed:11466275}.
CC -!- SIMILARITY: Belongs to the EcpR/MatA family. {ECO:0000305}.
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DR EMBL; U73857; AAB18023.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73397.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76078.1; -; Genomic_DNA.
DR PIR; F64755; F64755.
DR RefSeq; NP_414828.1; NC_000913.3.
DR AlphaFoldDB; P71301; -.
DR SMR; P71301; -.
DR BioGRID; 4259793; 6.
DR BioGRID; 849363; 1.
DR ComplexPortal; CPX-5764; matA-rcsB DNA-binding transcription factor complex.
DR IntAct; P71301; 2.
DR STRING; 511145.b0294; -.
DR PaxDb; P71301; -.
DR PRIDE; P71301; -.
DR EnsemblBacteria; AAC73397; AAC73397; b0294.
DR EnsemblBacteria; BAE76078; BAE76078; BAE76078.
DR GeneID; 944966; -.
DR KEGG; ecj:JW5031; -.
DR KEGG; eco:b0294; -.
DR PATRIC; fig|511145.12.peg.298; -.
DR EchoBASE; EB4070; -.
DR eggNOG; COG2771; Bacteria.
DR HOGENOM; CLU_128111_0_0_6; -.
DR OMA; CIWPAHD; -.
DR PhylomeDB; P71301; -.
DR BioCyc; EcoCyc:G6165-MON; -.
DR PRO; PR:P71301; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005667; C:transcription regulator complex; IPI:ComplexPortal.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:1902021; P:regulation of bacterial-type flagellum-dependent cell motility; IC:ComplexPortal.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:ComplexPortal.
DR CDD; cd06170; LuxR_C_like; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF00196; GerE; 1.
DR PRINTS; PR00038; HTHLUXR.
DR SMART; SM00421; HTH_LUXR; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR PROSITE; PS50043; HTH_LUXR_2; 1.
PE 1: Evidence at protein level;
KW Activator; Cytoplasm; DNA-binding; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..196
FT /note="HTH-type transcriptional regulator EcpR"
FT /id="PRO_0000184199"
FT DOMAIN 138..196
FT /note="HTH luxR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT DNA_BIND 162..181
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT MUTAGEN 179
FT /note="H->P: Slightly increases motility."
FT /evidence="ECO:0000269|PubMed:22422754"
SQ SEQUENCE 196 AA; 23274 MW; 5A3676E17E9793B9 CRC64;
MTWQSDYSRD YEVKNHMECQ NRSDKYIWSP HDAYFYKGLS ELIVDIDRLI YLSLEKIRKD
FVFINLSTDS LSEFINRDNE WLSAVKGKQV VLIAARKSEA LANYWYYNSN IRGVVYAGLS
RDIRKELVYV INGRFLRKDI KKDKITDREM EIIRMTAQGM QPKSIARIEN CSVKTVYTHR
RNAEAKLYSK IYKLVQ
