ECP_RAT
ID ECP_RAT Reviewed; 155 AA.
AC P70709;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Eosinophil cationic protein;
DE Short=ECP;
DE EC=3.1.27.-;
DE AltName: Full=Eosinophil secondary granule ribonuclease 11;
DE Short=EAR-11;
DE AltName: Full=Ribonuclease 3;
DE Short=RNase 3;
DE Flags: Precursor;
GN Name=Rnase3; Synonyms=Ear11, Rns3;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Sprague-Dawley; TISSUE=Bone marrow;
RX PubMed=9116043; DOI=10.1016/s0167-4781(97)00024-9;
RA Nittoh T., Hirakata M., Mue S., Ohuchi K.;
RT "Identification of cDNA encoding rat eosinophil cationic
RT protein/eosinophil-associated ribonuclease.";
RL Biochim. Biophys. Acta 1351:42-46(1997).
CC -!- FUNCTION: Cytotoxin and helminthotoxin with ribonuclease activity.
CC Possesses a wide variety of biological activities (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic granule {ECO:0000250}. Note=Matrix of
CC eosinophil's large specific granule. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the pancreatic ribonuclease family.
CC {ECO:0000305}.
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DR EMBL; D88586; BAA13648.1; -; mRNA.
DR RefSeq; NP_620257.1; NM_138902.1.
DR RefSeq; XP_002728238.1; XM_002728192.4.
DR AlphaFoldDB; P70709; -.
DR SMR; P70709; -.
DR STRING; 10116.ENSRNOP00000044390; -.
DR GlyGen; P70709; 2 sites.
DR PaxDb; P70709; -.
DR PRIDE; P70709; -.
DR Ensembl; ENSRNOT00000105605; ENSRNOP00000076490; ENSRNOG00000068464.
DR Ensembl; ENSRNOT00000119723; ENSRNOP00000084145; ENSRNOG00000064658.
DR GeneID; 100361866; -.
DR GeneID; 192264; -.
DR KEGG; rno:100361866; -.
DR KEGG; rno:192264; -.
DR CTD; 6037; -.
DR RGD; 621580; Rnase3.
DR eggNOG; ENOG502TF52; Eukaryota.
DR GeneTree; ENSGT00940000162253; -.
DR HOGENOM; CLU_117006_0_1_1; -.
DR InParanoid; P70709; -.
DR OMA; PSCNIAM; -.
DR OrthoDB; 1482425at2759; -.
DR PhylomeDB; P70709; -.
DR TreeFam; TF333393; -.
DR Reactome; R-RNO-6798695; Neutrophil degranulation.
DR PRO; PR:P70709; -.
DR Proteomes; UP000002494; Chromosome 15.
DR Bgee; ENSRNOG00000031445; Expressed in lung and 5 other tissues.
DR ExpressionAtlas; P70709; baseline.
DR Genevisible; P70709; RN.
DR GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR GO; GO:0005615; C:extracellular space; ISO:RGD.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0001530; F:lipopolysaccharide binding; ISO:RGD.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0004540; F:ribonuclease activity; IBA:GO_Central.
DR GO; GO:0019731; P:antibacterial humoral response; ISO:RGD.
DR GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; ISO:RGD.
DR GO; GO:0006935; P:chemotaxis; IBA:GO_Central.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; ISO:RGD.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; ISO:RGD.
DR GO; GO:0043152; P:induction of bacterial agglutination; ISO:RGD.
DR GO; GO:0045087; P:innate immune response; ISO:RGD.
DR GO; GO:0002227; P:innate immune response in mucosa; ISO:RGD.
DR Gene3D; 3.10.130.10; -; 1.
DR InterPro; IPR001427; RNaseA.
DR InterPro; IPR036816; RNaseA-like_dom_sf.
DR InterPro; IPR023411; RNaseA_AS.
DR InterPro; IPR023412; RNaseA_domain.
DR PANTHER; PTHR11437; PTHR11437; 1.
DR Pfam; PF00074; RnaseA; 1.
DR PRINTS; PR00794; RIBONUCLEASE.
DR SMART; SM00092; RNAse_Pc; 1.
DR SUPFAM; SSF54076; SSF54076; 1.
DR PROSITE; PS00127; RNASE_PANCREATIC; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Endonuclease; Glycoprotein; Hydrolase; Nuclease;
KW Reference proteome; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..155
FT /note="Eosinophil cationic protein"
FT /id="PRO_0000030866"
FT ACT_SITE 38
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT ACT_SITE 150
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT BINDING 62..66
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT CARBOHYD 88
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 107
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 47..106
FT /evidence="ECO:0000250"
FT DISULFID 61..118
FT /evidence="ECO:0000250"
FT DISULFID 79..133
FT /evidence="ECO:0000250"
FT DISULFID 86..94
FT /evidence="ECO:0000250"
SQ SEQUENCE 155 AA; 18007 MW; F190864C34EA22C7 CRC64;
MGLKLLESRL CLLLSLGLVL MLASCQPPTP SQWFEIQHIY NRAYPRCNDA MRHRNRFTGH
CKDINTFLHT SFASVVGVCG NRNIPCGNRT YRNCHNSRYR VSITFCNLTT PARIYTQCRY
QTTRSRKFYT VGCDPRTPRD SPMYPVVPVH LDRIF
