ID   ECSIT_DROME             Reviewed;         409 AA.
AC   Q9U6M0; Q8SZF7; Q9VNJ6;
DT   26-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   26-JUN-2007, sequence version 2.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial;
DE   Flags: Precursor;
GN   Name=ECSIT; ORFNames=CG10610;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND INTERACTION WITH TRAF6.
RX   PubMed=10465784; DOI=10.1101/gad.13.16.2059;
RA   Kopp E., Medzhitov R., Carothers J., Xiao C., Douglas I., Janeway C.A.,
RA   Ghosh S.;
RT   "ECSIT is an evolutionarily conserved intermediate in the Toll/IL-1 signal
RT   transduction pathway.";
RL   Genes Dev. 13:2059-2071(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Berkeley; TISSUE=Embryo;
RX   PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA   Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA   Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA   Celniker S.E.;
RT   "A Drosophila full-length cDNA resource.";
RL   Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC   -!- FUNCTION: Involved in the innate immune response. Promotes the
CC       production of antibacterial peptides. {ECO:0000269|PubMed:10465784}.
CC   -!- FUNCTION: Required for efficient assembly of mitochondrial
CC       NADH:ubiquinone oxidoreductase. {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with Traf6. {ECO:0000269|PubMed:10465784}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC       Mitochondrion {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ECSIT family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAF01220.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AF182511; AAF01220.1; ALT_FRAME; mRNA.
DR   EMBL; AE014297; AAF51937.1; -; Genomic_DNA.
DR   EMBL; AY070908; AAL48530.1; -; mRNA.
DR   RefSeq; NP_649602.2; NM_141345.2.
DR   AlphaFoldDB; Q9U6M0; -.
DR   BioGRID; 65936; 6.
DR   DIP; DIP-18542N; -.
DR   IntAct; Q9U6M0; 4.
DR   STRING; 7227.FBpp0078296; -.
DR   PaxDb; Q9U6M0; -.
DR   PRIDE; Q9U6M0; -.
DR   DNASU; 40732; -.
DR   EnsemblMetazoa; FBtr0078647; FBpp0078296; FBgn0028436.
DR   GeneID; 40732; -.
DR   KEGG; dme:Dmel_CG10610; -.
DR   UCSC; CG10610-RA; d. melanogaster.
DR   CTD; 51295; -.
DR   FlyBase; FBgn0028436; ECSIT.
DR   VEuPathDB; VectorBase:FBgn0028436; -.
DR   eggNOG; KOG3941; Eukaryota.
DR   GeneTree; ENSGT00390000005147; -.
DR   HOGENOM; CLU_046917_1_0_1; -.
DR   InParanoid; Q9U6M0; -.
DR   OMA; FGQHNVH; -.
DR   OrthoDB; 995360at2759; -.
DR   PhylomeDB; Q9U6M0; -.
DR   Reactome; R-DME-6799198; Complex I biogenesis.
DR   BioGRID-ORCS; 40732; 0 hits in 1 CRISPR screen.
DR   GenomeRNAi; 40732; -.
DR   PRO; PR:Q9U6M0; -.
DR   Proteomes; UP000000803; Chromosome 3R.
DR   Bgee; FBgn0028436; Expressed in adult hindgut (Drosophila) and 23 other tissues.
DR   ExpressionAtlas; Q9U6M0; baseline and differential.
DR   Genevisible; Q9U6M0; DM.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005739; C:mitochondrion; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0045087; P:innate immune response; IDA:FlyBase.
DR   GO; GO:0051341; P:regulation of oxidoreductase activity; ISS:UniProtKB.
DR   GO; GO:0061635; P:regulation of protein complex stability; ISS:UniProtKB.
DR   InterPro; IPR029342; ECIST_C.
DR   InterPro; IPR010418; ECSIT.
DR   PANTHER; PTHR13113; PTHR13113; 1.
DR   Pfam; PF06239; ECSIT; 1.
DR   Pfam; PF14784; ECSIT_C; 1.
DR   SMART; SM01284; ECSIT_Cterm; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Immunity; Innate immunity; Mitochondrion; Nucleus;
KW   Reference proteome; Transit peptide.
FT   TRANSIT         1..26
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..409
FT                   /note="Evolutionarily conserved signaling intermediate in
FT                   Toll pathway, mitochondrial"
FT                   /id="PRO_0000291992"
FT   REGION          27..53
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          383..409
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        28..45
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        120
FT                   /note="K -> R (in Ref. 4; AAL48530)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   409 AA;  47065 MW;  1F34CB7FF8A805CB CRC64;
     MLRRAQCLLR LHGNGGHSLV SRFRNYATDE GNPKQNPNPN PRAQKPGTKN LPALRNPFAA
     AQDRTKNSYL TMVEIFQERD VHRRNHVEFI YAALKNMADF GVERDLEVYK ALINVMPKGK
     FIPTNMFQAE FMHYPKQQQC IIDLLEQMED CGVMPDHEME AMLLNVFGRQ GHPLRKYWRM
     MYWMPKFKNL SPWPLPDPVP DDTLEMAKLA LERMCTVDLR SKITVFETSE LKDAIDDTWI
     VSGMSPEQEK LLREHSRQKA LYIEGPFHIW LRNRRINYFT LRADADSEFL SELDERQLDE
     DDVSHIEVPF FGRAPPRRHN QLGKLRSVHQ QDDGTIMAIC ATGTSTKDSL LSWIRLLEAN
     GNPSIGEVPV LFRFTSEVPA KAEEIEGGAS VPATSDNSSQ DEHISSRQK