ID   ECTC_MARHA              Reviewed;         129 AA.
AC   O06061;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=L-ectoine synthase;
DE            EC=4.2.1.108;
DE   AltName: Full=N-acetyldiaminobutyrate dehydratase;
GN   Name=ectC;
OS   Marinococcus halophilus.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Marinococcus.
OX   NCBI_TaxID=1371;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=ATCC 27964 / DSM 20408 / CIP 140819 / JCM 2479 / NBRC 102359 / NCIMB
RC   13496 / CCM 2706;
RX   PubMed=9141677; DOI=10.1099/00221287-143-4-1141;
RA   Louis P., Galinski E.A.;
RT   "Characterization of genes for the biosynthesis of the compatible solute
RT   ectoine from Marinococcus halophilus and osmoregulated expression in
RT   Escherichia coli.";
RL   Microbiology 143:1141-1149(1997).
CC   -!- FUNCTION: Catalyzes the circularization of gamma-N-acetyl-alpha,gamma-
CC       diaminobutyric acid (ADABA) to ectoine (1,4,5,6-tetrahydro-2-methyl-4-
CC       pyrimidine carboxylic acid), which is an excellent osmoprotectant.
CC       {ECO:0000269|PubMed:9141677}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2S)-4-acetamido-2-aminobutanoate = H2O + L-ectoine;
CC         Xref=Rhea:RHEA:17281, ChEBI:CHEBI:15377, ChEBI:CHEBI:58515,
CC         ChEBI:CHEBI:58929; EC=4.2.1.108;
CC   -!- PATHWAY: Amine and polyamine biosynthesis; ectoine biosynthesis; L-
CC       ectoine from L-aspartate 4-semialdehyde: step 3/3.
CC   -!- SIMILARITY: Belongs to the ectoine synthase family. {ECO:0000305}.
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DR   EMBL; U66614; AAB57635.1; -; Genomic_DNA.
DR   RefSeq; WP_022795050.1; NZ_NPFA01000031.1.
DR   AlphaFoldDB; O06061; -.
DR   SMR; O06061; -.
DR   STRING; 1371.GCA_900166605_00959; -.
DR   KEGG; ag:AAB57635; -.
DR   BRENDA; 4.2.1.108; 8055.
DR   UniPathway; UPA00067; UER00123.
DR   GO; GO:0033990; F:ectoine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019491; P:ectoine biosynthetic process; IEA:UniProtKB-UniRule.
DR   CDD; cd06978; cupin_EctC; 1.
DR   Gene3D; 2.60.120.10; -; 1.
DR   HAMAP; MF_01255; Ectoine_synth; 1.
DR   InterPro; IPR010462; Ectoine_synth.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   PANTHER; PTHR39289; PTHR39289; 1.
DR   Pfam; PF06339; Ectoine_synth; 1.
DR   SUPFAM; SSF51182; SSF51182; 1.
PE   3: Inferred from homology;
KW   Lyase.
FT   CHAIN           1..129
FT                   /note="L-ectoine synthase"
FT                   /id="PRO_0000220153"
SQ   SEQUENCE   129 AA;  14797 MW;  DDA07304470A200C CRC64;
     MKVIKLEDLL GTEREVDDGN WVSRRFIMKD DNMGYSVNDT IIRAGTETHI WYQNHLETVY
     CIEGDGEIET LSDNKVYQLE PGVLYALDKN DEHMLRGGSK DMRMVCVFNP PLSGREVHDE
     NGVYPADLD