ID   ECTC_SPOPA              Reviewed;         134 AA.
AC   Q9AP33;
DT   01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=L-ectoine synthase;
DE            EC=4.2.1.108;
DE   AltName: Full=N-acetyldiaminobutyrate dehydratase;
GN   Name=ectC;
OS   Sporosarcina pasteurii (Bacillus pasteurii).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Planococcaceae; Sporosarcina.
OX   NCBI_TaxID=1474;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND INDUCTION.
RC   STRAIN=ATCC 11859 / DSM 33 / NCIB 8841 / NCTC 4822;
RX   PubMed=11823218; DOI=10.1128/aem.68.2.772-783.2002;
RA   Kuhlmann A.U., Bremer E.;
RT   "Osmotically regulated synthesis of the compatible solute ectoine in
RT   Bacillus pasteurii and related Bacillus spp.";
RL   Appl. Environ. Microbiol. 68:772-783(2002).
CC   -!- FUNCTION: Catalyzes the circularization of gamma-N-acetyl-alpha,gamma-
CC       diaminobutyric acid (ADABA) to ectoine (1,4,5,6-tetrahydro-2-methyl-4-
CC       pyrimidine carboxylic acid), which is an excellent osmoprotectant.
CC       {ECO:0000305|PubMed:11823218}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2S)-4-acetamido-2-aminobutanoate = H2O + L-ectoine;
CC         Xref=Rhea:RHEA:17281, ChEBI:CHEBI:15377, ChEBI:CHEBI:58515,
CC         ChEBI:CHEBI:58929; EC=4.2.1.108;
CC   -!- PATHWAY: Amine and polyamine biosynthesis; ectoine biosynthesis; L-
CC       ectoine from L-aspartate 4-semialdehyde: step 3/3.
CC   -!- INDUCTION: By osmotic stress. {ECO:0000269|PubMed:11823218}.
CC   -!- SIMILARITY: Belongs to the ectoine synthase family. {ECO:0000305}.
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DR   EMBL; AF316874; AAK12085.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9AP33; -.
DR   SMR; Q9AP33; -.
DR   KEGG; ag:AAK12085; -.
DR   BRENDA; 4.2.1.108; 682.
DR   UniPathway; UPA00067; UER00123.
DR   GO; GO:0033990; F:ectoine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019491; P:ectoine biosynthetic process; IEA:UniProtKB-UniRule.
DR   CDD; cd06978; cupin_EctC; 1.
DR   Gene3D; 2.60.120.10; -; 1.
DR   HAMAP; MF_01255; Ectoine_synth; 1.
DR   InterPro; IPR010462; Ectoine_synth.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   PANTHER; PTHR39289; PTHR39289; 1.
DR   Pfam; PF06339; Ectoine_synth; 1.
DR   SUPFAM; SSF51182; SSF51182; 1.
PE   2: Evidence at transcript level;
KW   Lyase; Stress response.
FT   CHAIN           1..134
FT                   /note="L-ectoine synthase"
FT                   /id="PRO_0000220146"
SQ   SEQUENCE   134 AA;  15086 MW;  8610F9A0D126DA48 CRC64;
     MIVRTIDEII GTENEVESDT WTSRRLLLEK DGMGFSFHET IIYAGTETHI HYQNHLEAVY
     CVGGDGEIET VSDGKVYPIQ DGTMYALDQH DEHYPRGGKT DMRLICTFNP PLVGTETHDE
     NGVYPLLSKQ PVGK