ID   ECTC_VIBC3              Reviewed;         138 AA.
AC   A5F0H9; C3M6B7;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-JUN-2007, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=L-ectoine synthase {ECO:0000255|HAMAP-Rule:MF_01255};
DE            EC=4.2.1.108 {ECO:0000255|HAMAP-Rule:MF_01255};
DE   AltName: Full=N-acetyldiaminobutyrate dehydratase {ECO:0000255|HAMAP-Rule:MF_01255};
GN   Name=ectC {ECO:0000255|HAMAP-Rule:MF_01255};
GN   OrderedLocusNames=VC0395_0411, VC395_A0847;
OS   Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 /
OS   O395).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=345073;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RA   Heidelberg J.;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX   PubMed=19115014; DOI=10.1371/journal.pone.0004053;
RA   Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J.,
RA   Wang W., Wang J., Qian W., Li D., Wang L.;
RT   "A recalibrated molecular clock and independent origins for the cholera
RT   pandemic clones.";
RL   PLoS ONE 3:E4053-E4053(2008).
CC   -!- FUNCTION: Catalyzes the circularization of gamma-N-acetyl-alpha,gamma-
CC       diaminobutyric acid (ADABA) to ectoine (1,4,5,6-tetrahydro-2-methyl-4-
CC       pyrimidine carboxylic acid), which is an excellent osmoprotectant.
CC       {ECO:0000255|HAMAP-Rule:MF_01255}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2S)-4-acetamido-2-aminobutanoate = H2O + L-ectoine;
CC         Xref=Rhea:RHEA:17281, ChEBI:CHEBI:15377, ChEBI:CHEBI:58515,
CC         ChEBI:CHEBI:58929; EC=4.2.1.108; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01255};
CC   -!- PATHWAY: Amine and polyamine biosynthesis; ectoine biosynthesis; L-
CC       ectoine from L-aspartate 4-semialdehyde: step 3/3. {ECO:0000255|HAMAP-
CC       Rule:MF_01255}.
CC   -!- SIMILARITY: Belongs to the ectoine synthase family. {ECO:0000255|HAMAP-
CC       Rule:MF_01255}.
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DR   EMBL; CP000626; ABQ19414.1; -; Genomic_DNA.
DR   EMBL; CP001236; ACP11680.1; -; Genomic_DNA.
DR   RefSeq; WP_000637098.1; NZ_JAACZH010000012.1.
DR   AlphaFoldDB; A5F0H9; -.
DR   SMR; A5F0H9; -.
DR   STRING; 345073.VC395_A0847; -.
DR   EnsemblBacteria; ABQ19414; ABQ19414; VC0395_0411.
DR   GeneID; 57742201; -.
DR   KEGG; vco:VC0395_0411; -.
DR   KEGG; vcr:VC395_A0847; -.
DR   PATRIC; fig|345073.21.peg.3578; -.
DR   eggNOG; COG0662; Bacteria.
DR   HOGENOM; CLU_154525_0_0_6; -.
DR   OMA; CVFNPPI; -.
DR   UniPathway; UPA00067; UER00123.
DR   Proteomes; UP000000249; Chromosome 1.
DR   GO; GO:0033990; F:ectoine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019491; P:ectoine biosynthetic process; IEA:UniProtKB-UniRule.
DR   CDD; cd06978; cupin_EctC; 1.
DR   Gene3D; 2.60.120.10; -; 1.
DR   HAMAP; MF_01255; Ectoine_synth; 1.
DR   InterPro; IPR010462; Ectoine_synth.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   PANTHER; PTHR39289; PTHR39289; 1.
DR   Pfam; PF06339; Ectoine_synth; 1.
DR   SUPFAM; SSF51182; SSF51182; 1.
PE   3: Inferred from homology;
KW   Lyase.
FT   CHAIN           1..138
FT                   /note="L-ectoine synthase"
FT                   /id="PRO_1000073178"
SQ   SEQUENCE   138 AA;  15996 MW;  500962E3FEBF8F05 CRC64;
     MIVRTLEECR QSERRVVAEN WESVRMLLKD DHMGFSFHIT TIYANTQTHI HYRNHLESVY
     CMSGEGEIEV VGGKTYPIQP GTLYILDQHD EHYLRAFSSE MVMACVFNPP LTGHEIHDAE
     GVYPLDKSEL ISQCHKEK