ID   ECTT_VIRPA              Reviewed;         501 AA.
AC   Q93AK1;
DT   20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-MAY-2022, entry version 45.
DE   RecName: Full=Ectoine/hydroxyectoine transporter {ECO:0000303|PubMed:21764932};
GN   Name=ectT {ECO:0000303|PubMed:21764932};
OS   Virgibacillus pantothenticus.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Virgibacillus.
OX   NCBI_TaxID=1473;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES,
RP   SUBCELLULAR LOCATION, AND INDUCTION.
RC   STRAIN=DSM 26;
RX   PubMed=21764932; DOI=10.1128/jb.05270-11;
RA   Kuhlmann A.U., Hoffmann T., Bursy J., Jebbar M., Bremer E.;
RT   "Ectoine and hydroxyectoine as protectants against osmotic and cold stress:
RT   uptake through the SigB-controlled betaine-choline-carnitine transporter-
RT   type carrier EctT from Virgibacillus pantothenticus.";
RL   J. Bacteriol. 193:4699-4708(2011).
CC   -!- FUNCTION: Mediates the import of ectoine and hydroxyectoine, which
CC       function as osmotic and cold stress protectants. Has also minor uptake
CC       activities for the compatible solutes proline and glycine betaine.
CC       {ECO:0000269|PubMed:21764932}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=44 uM for ectoine {ECO:0000269|PubMed:21764932};
CC         Vmax=169 nmol/min/mg enzyme with ectoine as substrate
CC         {ECO:0000269|PubMed:21764932};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000305|PubMed:21764932}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- INDUCTION: Induced by high salinity and cold stress. Induction is
CC       mediated by SigB, the master regulator of the general stress response.
CC       {ECO:0000269|PubMed:21764932}.
CC   -!- SIMILARITY: Belongs to the BCCT transporter (TC 2.A.15) family.
CC       {ECO:0000305}.
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DR   EMBL; AF421189; AAL16076.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q93AK1; -.
DR   SMR; Q93AK1; -.
DR   TCDB; 2.A.15.1.8; the betaine/carnitine/choline transporter (bcct) family.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0071705; P:nitrogen compound transport; IEA:InterPro.
DR   InterPro; IPR000060; BCCT_transptr.
DR   PANTHER; PTHR30047; PTHR30047; 1.
DR   Pfam; PF02028; BCCT; 1.
DR   TIGRFAMs; TIGR00842; bcct; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Membrane; Stress response;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..501
FT                   /note="Ectoine/hydroxyectoine transporter"
FT                   /id="PRO_0000435358"
FT   TRANSMEM        9..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        45..65
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        86..106
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        137..157
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        190..210
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        220..240
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        258..278
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        311..331
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        343..363
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        395..415
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        441..461
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        465..485
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   501 AA;  54847 MW;  FA6A822A42238E44 CRC64;
     MNKSTLNNPV FYVSAFVVFL LVIIGATLPN RFGAVAEKLF HFTTIHFGWF YLLAVFVFVV
     FLITLSLSKF GKIKLGATLT KPEYSFFTWI GMLFSAGFGA GLVFWGVAEP MSHFFKTPFP
     AVEAMSEEAA RVAMGYAFFH WGVSQWSVFA IVGLVIAYLQ FRKKRRGLIS TSIQPIIGKN
     KFIADTVDSL AVIATVMGVA TSLGLGILQM NGGLKSVFDV PTSIWVQMAI AGVMLITYLI
     SSSTGLDRGI KWLSNINLGS LFIIIVFVFM AGPTVFILNT FVLGLGDYFS NFIGYSLRLT
     PYTGDTWVRE WTIFYWAWST AWSPFVGAFI ARVSRGRSIR QYVLGVLVVS PAIACIWIAA
     FGGTAVYNDL MNGTSIAEAV NADIAVALFE TYQHLPMTTI LSILSIFLIF TFLVTSADSA
     TYILGVMTSR GSLNPTLVTK IVWGLLITAI AVVLLLAGGL EALQTASLIS ALPFTVILLL
     MMASFTRMLS KGEKKAEQDK E