EDA2_ARATH
ID EDA2_ARATH Reviewed; 489 AA.
AC Q1PF50; Q9SL39;
DT 08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT 08-FEB-2011, sequence version 2.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Probable serine protease EDA2;
DE EC=3.4.-.-;
DE AltName: Full=Protein EMBRYO SAC DEVELOPMENT ARREST 2;
DE Flags: Precursor;
GN Name=EDA2; OrderedLocusNames=At2g18080; ORFNames=T27K22.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 73-489.
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [4]
RP PREDICTION.
RX PubMed=15333753; DOI=10.1104/pp.104.043695;
RA Reumann S., Ma C., Lemke S., Babujee L.;
RT "AraPerox. A database of putative Arabidopsis proteins from plant
RT peroxisomes.";
RL Plant Physiol. 136:2587-2608(2004).
RN [5]
RP FUNCTION.
RX PubMed=15634699; DOI=10.1242/dev.01595;
RA Pagnussat G.C., Yu H.-J., Ngo Q.A., Rajani S., Mayalagu S., Johnson C.S.,
RA Capron A., Xie L.-F., Ye D., Sundaresan V.;
RT "Genetic and molecular identification of genes required for female
RT gametophyte development and function in Arabidopsis.";
RL Development 132:603-614(2005).
RN [6]
RP DISRUPTION PHENOTYPE.
RX PubMed=19748917; DOI=10.1104/pp.109.142505;
RA Lingard M.J., Bartel B.;
RT "Arabidopsis LON2 is necessary for peroxisomal function and sustained
RT matrix protein import.";
RL Plant Physiol. 151:1354-1365(2009).
CC -!- FUNCTION: May be involved in a proteolytic pathway controlling the
CC nuclear division phase of megagametogenesis.
CC {ECO:0000269|PubMed:15634699}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}. Note=Prediction of a
CC peroxisomal location. {ECO:0000269|PubMed:15333753}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype.
CC {ECO:0000269|PubMed:19748917}.
CC -!- SIMILARITY: Belongs to the peptidase S28 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD20118.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC006201; AAD20118.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC06724.1; -; Genomic_DNA.
DR EMBL; DQ446516; ABE65823.1; -; mRNA.
DR PIR; A84560; A84560.
DR RefSeq; NP_179399.5; NM_127364.6.
DR AlphaFoldDB; Q1PF50; -.
DR SMR; Q1PF50; -.
DR STRING; 3702.AT2G18080.1; -.
DR MEROPS; S28.A05; -.
DR iPTMnet; Q1PF50; -.
DR PaxDb; Q1PF50; -.
DR PRIDE; Q1PF50; -.
DR ProteomicsDB; 222058; -.
DR EnsemblPlants; AT2G18080.1; AT2G18080.1; AT2G18080.
DR GeneID; 816320; -.
DR Gramene; AT2G18080.1; AT2G18080.1; AT2G18080.
DR KEGG; ath:AT2G18080; -.
DR Araport; AT2G18080; -.
DR TAIR; locus:2060959; AT2G18080.
DR eggNOG; KOG2182; Eukaryota.
DR HOGENOM; CLU_020959_4_1_1; -.
DR InParanoid; Q1PF50; -.
DR OMA; ACYNRIE; -.
DR OrthoDB; 555765at2759; -.
DR PhylomeDB; Q1PF50; -.
DR PRO; PR:Q1PF50; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q1PF50; baseline and differential.
DR Genevisible; Q1PF50; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008239; F:dipeptidyl-peptidase activity; IBA:GO_Central.
DR GO; GO:0008236; F:serine-type peptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0009561; P:megagametogenesis; IMP:TAIR.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 1.20.120.980; -; 1.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR008758; Peptidase_S28.
DR InterPro; IPR042269; Ser_carbopepase_S28_SKS.
DR Pfam; PF05577; Peptidase_S28; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Hydrolase; Protease; Reference proteome; Secreted;
KW Serine protease; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..489
FT /note="Probable serine protease EDA2"
FT /id="PRO_0000405231"
FT ACT_SITE 178
FT /note="Charge relay system"
FT /evidence="ECO:0000255"
FT ACT_SITE 410
FT /note="Charge relay system"
FT /evidence="ECO:0000255"
FT ACT_SITE 436
FT /note="Charge relay system"
FT /evidence="ECO:0000255"
FT CARBOHYD 35
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 51
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 162
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 253
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 293
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 365
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 406
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 419
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 456
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 489 AA; 55807 MW; E284E3BA7C226F59 CRC64;
MSLEFGFILI NIFTAIVSFS TLSHALLHPS SVSHNVSRSR YYMTTNELWF NQTLDHESPN
DHRKFRQRYY EFMDYFRSPD GPMFMIICGE GPCSGIANDY INVLAKKFQA GVVSLEHRYY
GKSSPFNSLA TENLKYLSSK QALYDLASFR QYYQESLNKK LNISSGGSDN PWFFFGISYS
GALSAWFRLK FPHLTCGSLA SSAVVRAIYE FSEFDQQIGE SAGQECKLAL QETNKLLELG
LKVKNKAVKS LFNATELDVD ADFLYLTADA AVMAFQYGNP DKLCVPLVEA KKNGSDLVVT
YSTYVREYCM RIWGLRVRTY NRKHLRNTVV TADSAYRLWW FQACTELGYF QVAPKYDSVR
SHQINTTFHL DLCKSLFGKD VYPKVDATNL YYGGDRLAAT KIIFTNGSED PWRHASKQNS
THEMPSYIIK CRNCGHGSDI RGCPQSPMVI EGKSNNCSLP DYVNKVRQQM VEHIDLWLSE
CRQSIRSSI
