EDD_PSEAE
ID EDD_PSEAE Reviewed; 608 AA.
AC P31961; Q9HZ45;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 11-JAN-2001, sequence version 3.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Phosphogluconate dehydratase {ECO:0000255|HAMAP-Rule:MF_02094};
DE EC=4.2.1.12 {ECO:0000255|HAMAP-Rule:MF_02094};
DE AltName: Full=6-phosphogluconate dehydratase {ECO:0000303|PubMed:8045900};
GN Name=edd {ECO:0000255|HAMAP-Rule:MF_02094, ECO:0000303|PubMed:8045900};
GN OrderedLocusNames=PA3194;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=8045900; DOI=10.1128/jb.176.15.4700-4709.1994;
RA Temple L.M., Sage A., Christie G.E., Phibbs P.V. Jr.;
RT "Two genes for carbohydrate catabolism are divergently transcribed from a
RT region of DNA containing the hexC locus in Pseudomonas aeruginosa PAO1.";
RL J. Bacteriol. 176:4700-4709(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Catalyzes the dehydration of 6-phospho-D-gluconate to 2-
CC dehydro-3-deoxy-6-phospho-D-gluconate. {ECO:0000255|HAMAP-
CC Rule:MF_02094}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=6-phospho-D-gluconate = 2-dehydro-3-deoxy-6-phospho-D-
CC gluconate + H2O; Xref=Rhea:RHEA:17277, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:57569, ChEBI:CHEBI:58759; EC=4.2.1.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_02094};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_02094};
CC Note=Binds 1 [4Fe-4S] cluster. {ECO:0000255|HAMAP-Rule:MF_02094};
CC -!- PATHWAY: Carbohydrate metabolism; Entner-Doudoroff pathway.
CC {ECO:0000255|HAMAP-Rule:MF_02094}.
CC -!- SIMILARITY: Belongs to the IlvD/Edd family. {ECO:0000255|HAMAP-
CC Rule:MF_02094, ECO:0000305}.
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DR EMBL; M74256; AAA03487.1; -; Unassigned_DNA.
DR EMBL; AE004091; AAG06582.1; -; Genomic_DNA.
DR PIR; G83246; G83246.
DR RefSeq; NP_251884.1; NC_002516.2.
DR RefSeq; WP_003091478.1; NZ_QZGE01000019.1.
DR AlphaFoldDB; P31961; -.
DR SMR; P31961; -.
DR STRING; 287.DR97_4742; -.
DR PaxDb; P31961; -.
DR PRIDE; P31961; -.
DR EnsemblBacteria; AAG06582; AAG06582; PA3194.
DR GeneID; 882909; -.
DR KEGG; pae:PA3194; -.
DR PATRIC; fig|208964.12.peg.3340; -.
DR PseudoCAP; PA3194; -.
DR HOGENOM; CLU_014271_1_2_6; -.
DR InParanoid; P31961; -.
DR OMA; CANIAHV; -.
DR PhylomeDB; P31961; -.
DR BioCyc; PAER208964:G1FZ6-3254-MON; -.
DR UniPathway; UPA00226; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016836; F:hydro-lyase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004456; F:phosphogluconate dehydratase activity; IBA:GO_Central.
DR GO; GO:0046177; P:D-gluconate catabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0009255; P:Entner-Doudoroff pathway through 6-phosphogluconate; IEA:UniProtKB-UniRule.
DR Gene3D; 3.50.30.80; -; 1.
DR HAMAP; MF_02094; Edd; 1.
DR InterPro; IPR004786; 6-phosphgluc_deHydtase.
DR InterPro; IPR042096; Dihydro-acid_dehy_C.
DR InterPro; IPR000581; DiOHA_6PGluconate_deHydtase.
DR InterPro; IPR020558; DiOHA_6PGluconate_deHydtase_CS.
DR InterPro; IPR037237; IlvD/EDD_N.
DR PANTHER; PTHR43661:SF1; PTHR43661:SF1; 1.
DR Pfam; PF00920; ILVD_EDD; 1.
DR SUPFAM; SSF143975; SSF143975; 1.
DR TIGRFAMs; TIGR01196; edd; 1.
DR PROSITE; PS00886; ILVD_EDD_1; 1.
DR PROSITE; PS00887; ILVD_EDD_2; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; Carbohydrate metabolism; Gluconate utilization; Iron; Iron-sulfur;
KW Lyase; Metal-binding; Reference proteome.
FT CHAIN 1..608
FT /note="Phosphogluconate dehydratase"
FT /id="PRO_0000103557"
FT BINDING 154
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02094"
FT BINDING 221
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02094"
FT CONFLICT 84..88
FT /note="FERFP -> VRAPFR (in Ref. 1; AAA03487)"
FT /evidence="ECO:0000305"
FT CONFLICT 93..104
FT /note="QALHEIGSVGQF -> PGACTRSVRSASV (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 163..167
FT /note="IGSLR -> SAAS (in Ref. 1; AAA03487)"
FT /evidence="ECO:0000305"
FT CONFLICT 178
FT /note="A -> G (in Ref. 1; AAA03487)"
FT /evidence="ECO:0000305"
FT CONFLICT 232
FT /note="L -> VR (in Ref. 1; AAA03487)"
FT /evidence="ECO:0000305"
FT CONFLICT 258
FT /note="T -> S (in Ref. 1; AAA03487)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 608 AA; 65183 MW; 1C2A415B32B95911 CRC64;
MHPRVLEVTR RIQARSAATR QRYLEMVRAA ASKGPHRGTL PCGNLAHGVA ACGESDKQTL
RLMNQANVAI VSAYNDMLSA HQPFERFPGL IKQALHEIGS VGQFAGGVPA MCDGVTQGEP
GMELSLASRD VIAMSTAIAL SHNMFDAALC LGVCDKIVPG LLIGSLRFGH LPTVFVPAGP
MPTGISNKEK AAVRQLFAEG KATREELLAS EMASYHAPGT CTFYGTANTN QLLVEVMGLH
LPGASFVNPN TPLRDELTRE AARQASRLTP ENGNYVPMAE IVDEKAIVNS VVALLATGGS
TNHTLHLLAI AQAAGIQLTW QDMSELSHVV PTLARIYPNG QADINHFQAA GGMSFLIRQL
LDGGLLHEDV QTVAGPGLRR YTREPFLEDG RLVWREGPER SLDEAILRPL DKPFSAEGGL
RLMEGNLGRG VMKVSAVAPE HQVVEAPVRI FHDQASLAAA FKAGELERDL VAVVRFQGPR
ANGMPELHKL TPFLGVLQDR GFKVALVTDG RMSGASGKVP AAIHVSPEAI AGGPLARLRD
GDRVRVDGVN GELRVLVDDA EWQARSLEPA PQDGNLGCGR ELFAFMRNAM SSAEEGACSF
TESLNGWR
