3L21_NOTSC
ID 3L21_NOTSC Reviewed; 94 AA.
AC P01384; A8HDK3;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2009, sequence version 2.
DT 25-MAY-2022, entry version 93.
DE RecName: Full=Alpha-elapitoxin-Nss2a;
DE Short=Alpha-EPTX-Nss2a;
DE AltName: Full=Long neurotoxin 1;
DE Short=LNTX-1;
DE AltName: Full=Notechis III-4;
DE Flags: Precursor;
OS Notechis scutatus scutatus (Mainland tiger snake) (Common tiger snake).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC Serpentes; Colubroidea; Elapidae; Acanthophiinae; Notechis.
OX NCBI_TaxID=70142;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Venom gland;
RX PubMed=17906946; DOI=10.1007/s00018-007-7352-z;
RA St Pierre L., Fischer H., Adams D.J., Schenning M., Lavidis N.,
RA de Jersey J., Masci P.P., Lavin M.F.;
RT "Distinct activities of novel neurotoxins from Australian venomous snakes
RT for nicotinic acetylcholine receptors.";
RL Cell. Mol. Life Sci. 64:2829-2840(2007).
RN [2]
RP PROTEIN SEQUENCE OF 22-94, TOXIC DOSE, AND SUBCELLULAR LOCATION.
RC TISSUE=Venom;
RX PubMed=497256; DOI=10.1016/s0300-9084(79)80172-8;
RA Halpert J., Fohlman J., Eaker D.;
RT "Amino acid sequence of a postsynaptic neurotoxin from the venom of the
RT Australian tiger snake Notechis scutatus scutatus.";
RL Biochimie 61:719-723(1979).
CC -!- FUNCTION: Binds with high affinity to muscular (alpha-1/CHRNA1) and
CC neuronal (alpha-7/CHRNA7) nicotinic acetylcholine receptor (nAChR) and
CC inhibits acetylcholine from binding to the receptor, thereby impairing
CC neuromuscular and neuronal transmission.
CC {ECO:0000250|UniProtKB:P60615}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:497256}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC -!- TOXIC DOSE: LD(50) is 0.125 mg/kg by intravenous injection.
CC {ECO:0000269|PubMed:497256}.
CC -!- SIMILARITY: Belongs to the snake three-finger toxin family. Long-chain
CC subfamily. Type II alpha-neurotoxin sub-subfamily. {ECO:0000305}.
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DR EMBL; DQ917508; ABK63537.1; -; mRNA.
DR PIR; A01655; N2NO1.
DR AlphaFoldDB; P01384; -.
DR SMR; P01384; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IEA:UniProtKB-KW.
DR GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR CDD; cd00206; snake_toxin; 1.
DR Gene3D; 2.10.60.10; -; 1.
DR InterPro; IPR003571; Snake_3FTx.
DR InterPro; IPR045860; Snake_toxin-like_sf.
DR InterPro; IPR018354; Snake_toxin_con_site.
DR SUPFAM; SSF57302; SSF57302; 1.
DR PROSITE; PS00272; SNAKE_TOXIN; 1.
PE 1: Evidence at protein level;
KW Acetylcholine receptor inhibiting toxin; Direct protein sequencing;
KW Disulfide bond; Ion channel impairing toxin; Neurotoxin;
KW Postsynaptic neurotoxin; Secreted; Signal; Toxin.
FT SIGNAL 1..21
FT /evidence="ECO:0000269|PubMed:497256"
FT CHAIN 22..94
FT /note="Alpha-elapitoxin-Nss2a"
FT /id="PRO_0000093555"
FT DISULFID 24..41
FT /evidence="ECO:0000250"
FT DISULFID 34..62
FT /evidence="ECO:0000250"
FT DISULFID 47..51
FT /evidence="ECO:0000250"
FT DISULFID 66..77
FT /evidence="ECO:0000250"
FT DISULFID 78..83
FT /evidence="ECO:0000250"
FT CONFLICT 92..93
FT /note="PH -> HP (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 94 AA; 10289 MW; D316FCF1688AEEC9 CRC64;
MKTLLLTLVV VTIVCLDLGD SLICYMGPKT PRTCPRGQNL CYTKTWCDAF CSSRGKVVEL
GCAATCPIAK SYEDVTCCST DNCNPFPVRP RPHP
