EDN1_MOUSE
ID EDN1_MOUSE Reviewed; 202 AA.
AC P22387; P70203; P97338;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 3.
DT 03-AUG-2022, entry version 187.
DE RecName: Full=Endothelin-1;
DE Short=ET-1;
DE AltName: Full=Preproendothelin-1;
DE Short=PPET1;
DE Contains:
DE RecName: Full=Big endothelin-1;
DE Flags: Precursor;
GN Name=Edn1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6 X CBA; TISSUE=Lung;
RX PubMed=8575440; DOI=10.1111/j.1432-1033.1995.819_a.x;
RA Chan T.S., Lin C.X., Chan W.Y., Chung S.S., Chung S.K.;
RT "Mouse preproendothelin-1 gene. cDNA cloning, sequence analysis and
RT determination of sites of expression during embryonic development.";
RL Eur. J. Biochem. 234:819-826(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=BALB/cJ; TISSUE=Lung;
RX PubMed=8688455; DOI=10.1016/0167-4781(96)00066-8;
RA Kurama M., Ishida N., Matsui M., Saida K., Mitsui Y.;
RT "Sequence and neuronal expression of mouse endothelin-1 cDNA.";
RL Biochim. Biophys. Acta 1307:249-253(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=BALB/cJ; TISSUE=Liver;
RX PubMed=8824799; DOI=10.1006/geno.1996.0029;
RA Maemura K., Kurihara H., Kurihara Y., Oda H., Ishikawa T., Copeland N.G.,
RA Gilbert D.J., Jenkins N.A., Yazaki Y.;
RT "Sequence analysis, chromosomal location, and developmental expression of
RT the mouse preproendothelin-1 gene.";
RL Genomics 31:177-184(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N-3; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
RC STRAIN=BALB/cJ;
RX PubMed=7883980; DOI=10.1172/jci117784;
RA Harats D., Kurihara H., Belloni P., Oakley H., Ziober A., Ackley D.,
RA Cain G., Kurihara Y., Lawn R., Sigal E.;
RT "Targeting gene expression to the vascular wall in transgenic mice using
RT the murine preproendothelin-1 promoter.";
RL J. Clin. Invest. 95:1335-1344(1995).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 36-76.
RC TISSUE=Kidney;
RX PubMed=8238383; DOI=10.1152/ajprenal.1993.265.4.f542;
RA Chen M., Todd-Turla K., Wang W.H., Cao X., Smart A., Brosius F.C.,
RA Killen P.D., Keiser J.A., Briggs J.P., Schnermann J.;
RT "Endothelin-1 mRNA in glomerular and epithelial cells of kidney.";
RL Am. J. Physiol. 265:F542-F550(1993).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 48-77.
RX PubMed=2768235; DOI=10.1016/s0021-9258(18)63739-1;
RA Saida K., Mitsui Y., Ishida N.;
RT "A novel peptide, vasoactive intestinal contractor, of a new (endothelin)
RT peptide family. Molecular cloning, expression, and biological activity.";
RL J. Biol. Chem. 264:14613-14616(1989).
RN [8]
RP PROTEIN SEQUENCE OF 155-165, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=OF1; TISSUE=Hippocampus;
RA Lubec G., Sunyer B., Chen W.-Q.;
RL Submitted (JAN-2009) to UniProtKB.
RN [9]
RP FUNCTION.
RX PubMed=16293765; DOI=10.1152/physiolgenomics.00144.2005;
RA Herrera V.L., Ponce L.R., Bagamasbad P.D., VanPelt B.D., Didishvili T.,
RA Ruiz-Opazo N.;
RT "Embryonic lethality in Dear gene-deficient mice: new player in
RT angiogenesis.";
RL Physiol. Genomics 23:257-268(2005).
CC -!- FUNCTION: Endothelins are endothelium-derived vasoconstrictor peptides
CC (By similarity). Probable ligand for G-protein coupled receptors EDNRA
CC and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and
CC RHOA cascade in glomerular mesangial cells (By similarity). Also binds
CC the DEAR/FBXW7-AS1 receptor (PubMed:16293765).
CC {ECO:0000250|UniProtKB:P05305, ECO:0000250|UniProtKB:P09558,
CC ECO:0000269|PubMed:16293765}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Highest expression in the adult is in lung. Lower
CC levels found in heart, kidney, brain and intestine. In the embryo,
CC expressed in outer and inner pharyngeal arch surfaces. Also expressed
CC in endothelium of dorsal aorta and arch arteries, and in epithelium of
CC pharyngeal pouches. {ECO:0000269|PubMed:8824799}.
CC -!- DEVELOPMENTAL STAGE: Expression in lung is low at 17.5 dpc fetal mice
CC and increases progressively into adulthood.
CC {ECO:0000269|PubMed:8824799}.
CC -!- SIMILARITY: Belongs to the endothelin/sarafotoxin family.
CC {ECO:0000305}.
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DR EMBL; U35233; AAA97608.1; -; mRNA.
DR EMBL; D43775; BAA07830.1; -; mRNA.
DR EMBL; D70842; BAA11109.1; -; Genomic_DNA.
DR EMBL; BC029547; AAH29547.1; -; mRNA.
DR EMBL; U07982; AAA74439.1; ALT_SEQ; Genomic_DNA.
DR EMBL; S66650; AAP13983.1; -; mRNA.
DR EMBL; M26497; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS26474.1; -.
DR PIR; S63538; S63538.
DR RefSeq; NP_034234.1; NM_010104.4.
DR AlphaFoldDB; P22387; -.
DR BMRB; P22387; -.
DR STRING; 10090.ENSMUSP00000021796; -.
DR PhosphoSitePlus; P22387; -.
DR PaxDb; P22387; -.
DR PRIDE; P22387; -.
DR ProteomicsDB; 277682; -.
DR Antibodypedia; 10136; 802 antibodies from 38 providers.
DR DNASU; 13614; -.
DR Ensembl; ENSMUST00000021796; ENSMUSP00000021796; ENSMUSG00000021367.
DR GeneID; 13614; -.
DR KEGG; mmu:13614; -.
DR UCSC; uc007qfl.2; mouse.
DR CTD; 1906; -.
DR MGI; MGI:95283; Edn1.
DR VEuPathDB; HostDB:ENSMUSG00000021367; -.
DR eggNOG; ENOG502S1NV; Eukaryota.
DR GeneTree; ENSGT00950000183053; -.
DR HOGENOM; CLU_090013_1_0_1; -.
DR InParanoid; P22387; -.
DR OMA; RCQCANQ; -.
DR OrthoDB; 1264335at2759; -.
DR PhylomeDB; P22387; -.
DR TreeFam; TF333184; -.
DR Reactome; R-MMU-375276; Peptide ligand-binding receptors.
DR Reactome; R-MMU-416476; G alpha (q) signalling events.
DR BioGRID-ORCS; 13614; 0 hits in 71 CRISPR screens.
DR ChiTaRS; Edn1; mouse.
DR PRO; PR:P22387; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; P22387; protein.
DR Bgee; ENSMUSG00000021367; Expressed in trunk blood vessel and 199 other tissues.
DR ExpressionAtlas; P22387; baseline and differential.
DR Genevisible; P22387; MM.
DR GO; GO:0045178; C:basal part of cell; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005615; C:extracellular space; IDA:MGI.
DR GO; GO:0048237; C:rough endoplasmic reticulum lumen; ISO:MGI.
DR GO; GO:0033093; C:Weibel-Palade body; ISO:MGI.
DR GO; GO:0005125; F:cytokine activity; ISO:MGI.
DR GO; GO:0031707; F:endothelin A receptor binding; IPI:MGI.
DR GO; GO:0031708; F:endothelin B receptor binding; ISO:MGI.
DR GO; GO:0005179; F:hormone activity; ISO:MGI.
DR GO; GO:0005102; F:signaling receptor binding; IDA:MGI.
DR GO; GO:0007193; P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway; IDA:MGI.
DR GO; GO:0014824; P:artery smooth muscle contraction; ISO:MGI.
DR GO; GO:0048675; P:axon extension; IDA:MGI.
DR GO; GO:0060385; P:axonogenesis involved in innervation; IMP:MGI.
DR GO; GO:0048514; P:blood vessel morphogenesis; IMP:MGI.
DR GO; GO:0007589; P:body fluid secretion; IMP:MGI.
DR GO; GO:0001569; P:branching involved in blood vessel morphogenesis; IMP:MGI.
DR GO; GO:0070588; P:calcium ion transmembrane transport; IDA:MGI.
DR GO; GO:0019722; P:calcium-mediated signaling; ISO:MGI.
DR GO; GO:0060070; P:canonical Wnt signaling pathway; IDA:MGI.
DR GO; GO:0003253; P:cardiac neural crest cell migration involved in outflow tract morphogenesis; IDA:MGI.
DR GO; GO:0051216; P:cartilage development; IMP:MGI.
DR GO; GO:0007166; P:cell surface receptor signaling pathway; ISO:MGI.
DR GO; GO:0007267; P:cell-cell signaling; ISO:MGI.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; IDA:MGI.
DR GO; GO:0071277; P:cellular response to calcium ion; IEA:Ensembl.
DR GO; GO:0071398; P:cellular response to fatty acid; IEA:Ensembl.
DR GO; GO:0071372; P:cellular response to follicle-stimulating hormone stimulus; IDA:MGI.
DR GO; GO:0071385; P:cellular response to glucocorticoid stimulus; IEA:Ensembl.
DR GO; GO:0044751; P:cellular response to human chorionic gonadotropin stimulus; IDA:MGI.
DR GO; GO:0070301; P:cellular response to hydrogen peroxide; ISO:MGI.
DR GO; GO:0071456; P:cellular response to hypoxia; IEA:Ensembl.
DR GO; GO:0071346; P:cellular response to interferon-gamma; IEA:Ensembl.
DR GO; GO:0071347; P:cellular response to interleukin-1; IEA:Ensembl.
DR GO; GO:0071373; P:cellular response to luteinizing hormone stimulus; IDA:MGI.
DR GO; GO:0071389; P:cellular response to mineralocorticoid stimulus; IEA:Ensembl.
DR GO; GO:0071310; P:cellular response to organic substance; IDA:MGI.
DR GO; GO:0071560; P:cellular response to transforming growth factor beta stimulus; IEA:Ensembl.
DR GO; GO:0071356; P:cellular response to tumor necrosis factor; IEA:Ensembl.
DR GO; GO:0071466; P:cellular response to xenobiotic stimulus; IEA:Ensembl.
DR GO; GO:0009953; P:dorsal/ventral pattern formation; IMP:MGI.
DR GO; GO:0035050; P:embryonic heart tube development; IMP:MGI.
DR GO; GO:0086100; P:endothelin receptor signaling pathway; ISS:UniProtKB.
DR GO; GO:0086101; P:endothelin receptor signaling pathway involved in heart process; IDA:MGI.
DR GO; GO:0042045; P:epithelial fluid transport; ISO:MGI.
DR GO; GO:0070371; P:ERK1 and ERK2 cascade; IDA:MGI.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; ISO:MGI.
DR GO; GO:0010467; P:gene expression; IDA:MGI.
DR GO; GO:0007507; P:heart development; IMP:MGI.
DR GO; GO:0001821; P:histamine secretion; ISO:MGI.
DR GO; GO:0007249; P:I-kappaB kinase/NF-kappaB signaling; IDA:MGI.
DR GO; GO:0001701; P:in utero embryonic development; IMP:MGI.
DR GO; GO:0048016; P:inositol phosphate-mediated signaling; ISO:MGI.
DR GO; GO:0035556; P:intracellular signal transduction; ISO:MGI.
DR GO; GO:0000165; P:MAPK cascade; IDA:MGI.
DR GO; GO:0060137; P:maternal process involved in parturition; IEA:Ensembl.
DR GO; GO:1903537; P:meiotic cell cycle process involved in oocyte maturation; IDA:MGI.
DR GO; GO:0051899; P:membrane depolarization; ISO:MGI.
DR GO; GO:0042474; P:middle ear morphogenesis; IMP:MGI.
DR GO; GO:0007005; P:mitochondrion organization; IDA:MGI.
DR GO; GO:0010259; P:multicellular organism aging; IDA:MGI.
DR GO; GO:0010629; P:negative regulation of gene expression; ISO:MGI.
DR GO; GO:0046888; P:negative regulation of hormone secretion; ISO:MGI.
DR GO; GO:0051771; P:negative regulation of nitric-oxide synthase biosynthetic process; ISO:MGI.
DR GO; GO:0051248; P:negative regulation of protein metabolic process; ISO:MGI.
DR GO; GO:0034392; P:negative regulation of smooth muscle cell apoptotic process; ISO:MGI.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0014032; P:neural crest cell development; IMP:MGI.
DR GO; GO:0014033; P:neural crest cell differentiation; IMP:MGI.
DR GO; GO:0014034; P:neural crest cell fate commitment; IMP:MGI.
DR GO; GO:0031175; P:neuron projection development; IDA:MGI.
DR GO; GO:0030185; P:nitric oxide transport; ISO:MGI.
DR GO; GO:0003357; P:noradrenergic neuron differentiation; IMP:MGI.
DR GO; GO:0030072; P:peptide hormone secretion; ISO:MGI.
DR GO; GO:0061626; P:pharyngeal arch artery morphogenesis; IMP:MGI.
DR GO; GO:0014065; P:phosphatidylinositol 3-kinase signaling; ISO:MGI.
DR GO; GO:0031583; P:phospholipase D-activating G protein-coupled receptor signaling pathway; IDA:MGI.
DR GO; GO:0010613; P:positive regulation of cardiac muscle hypertrophy; ISO:MGI.
DR GO; GO:2001259; P:positive regulation of cation channel activity; ISO:MGI.
DR GO; GO:0061051; P:positive regulation of cell growth involved in cardiac muscle cell development; ISO:MGI.
DR GO; GO:0030335; P:positive regulation of cell migration; ISO:MGI.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IDA:MGI.
DR GO; GO:0045793; P:positive regulation of cell size; ISO:MGI.
DR GO; GO:0070101; P:positive regulation of chemokine-mediated signaling pathway; ISO:MGI.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; ISO:MGI.
DR GO; GO:0051482; P:positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling pathway; IDA:MGI.
DR GO; GO:0051091; P:positive regulation of DNA-binding transcription factor activity; ISO:MGI.
DR GO; GO:0010460; P:positive regulation of heart rate; ISO:MGI.
DR GO; GO:0046887; P:positive regulation of hormone secretion; ISO:MGI.
DR GO; GO:0043507; P:positive regulation of JUN kinase activity; ISO:MGI.
DR GO; GO:0043406; P:positive regulation of MAP kinase activity; ISO:MGI.
DR GO; GO:0045840; P:positive regulation of mitotic nuclear division; ISO:MGI.
DR GO; GO:0090023; P:positive regulation of neutrophil chemotaxis; ISO:MGI.
DR GO; GO:1901224; P:positive regulation of NIK/NF-kappaB signaling; ISO:MGI.
DR GO; GO:0042482; P:positive regulation of odontogenesis; ISO:MGI.
DR GO; GO:0032308; P:positive regulation of prostaglandin secretion; ISO:MGI.
DR GO; GO:0060585; P:positive regulation of prostaglandin-endoperoxide synthase activity; ISO:MGI.
DR GO; GO:0035815; P:positive regulation of renal sodium excretion; IMP:MGI.
DR GO; GO:0060298; P:positive regulation of sarcomere organization; ISO:MGI.
DR GO; GO:2000273; P:positive regulation of signaling receptor activity; ISO:MGI.
DR GO; GO:0048661; P:positive regulation of smooth muscle cell proliferation; ISO:MGI.
DR GO; GO:0045987; P:positive regulation of smooth muscle contraction; IDA:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0035810; P:positive regulation of urine volume; IMP:MGI.
DR GO; GO:1904707; P:positive regulation of vascular associated smooth muscle cell proliferation; ISO:MGI.
DR GO; GO:0001516; P:prostaglandin biosynthetic process; ISO:MGI.
DR GO; GO:0010737; P:protein kinase A signaling; ISO:MGI.
DR GO; GO:0042313; P:protein kinase C deactivation; ISO:MGI.
DR GO; GO:0007205; P:protein kinase C-activating G protein-coupled receptor signaling pathway; IDA:MGI.
DR GO; GO:0006468; P:protein phosphorylation; IDA:MGI.
DR GO; GO:0008217; P:regulation of blood pressure; IMP:MGI.
DR GO; GO:0010827; P:regulation of glucose transmembrane transport; IDA:MGI.
DR GO; GO:0006885; P:regulation of pH; IDA:MGI.
DR GO; GO:0051930; P:regulation of sensory perception of pain; ISO:MGI.
DR GO; GO:0003100; P:regulation of systemic arterial blood pressure by endothelin; ISO:MGI.
DR GO; GO:0019229; P:regulation of vasoconstriction; IEA:InterPro.
DR GO; GO:0070294; P:renal sodium ion absorption; IDA:MGI.
DR GO; GO:0007585; P:respiratory gaseous exchange by respiratory system; IMP:MGI.
DR GO; GO:0014823; P:response to activity; IEA:Ensembl.
DR GO; GO:0043200; P:response to amino acid; IEA:Ensembl.
DR GO; GO:0001975; P:response to amphetamine; IMP:MGI.
DR GO; GO:0071548; P:response to dexamethasone; IEA:Ensembl.
DR GO; GO:0001666; P:response to hypoxia; IMP:MGI.
DR GO; GO:0044321; P:response to leptin; IEA:Ensembl.
DR GO; GO:0032496; P:response to lipopolysaccharide; IEA:Ensembl.
DR GO; GO:0035994; P:response to muscle stretch; IEA:Ensembl.
DR GO; GO:0035094; P:response to nicotine; IEA:Ensembl.
DR GO; GO:0010193; P:response to ozone; IEA:Ensembl.
DR GO; GO:0034696; P:response to prostaglandin F; IEA:Ensembl.
DR GO; GO:1902074; P:response to salt; IEA:Ensembl.
DR GO; GO:0033574; P:response to testosterone; IEA:Ensembl.
DR GO; GO:0043179; P:rhythmic excitation; IMP:MGI.
DR GO; GO:1902287; P:semaphorin-plexin signaling pathway involved in axon guidance; IMP:MGI.
DR GO; GO:0019233; P:sensory perception of pain; ISO:MGI.
DR GO; GO:0001501; P:skeletal system development; IMP:MGI.
DR GO; GO:0006939; P:smooth muscle contraction; IDA:MGI.
DR GO; GO:0042554; P:superoxide anion generation; ISO:MGI.
DR GO; GO:0097492; P:sympathetic neuron axon guidance; IMP:MGI.
DR GO; GO:0030878; P:thyroid gland development; IMP:MGI.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0042310; P:vasoconstriction; ISO:MGI.
DR GO; GO:0014826; P:vein smooth muscle contraction; ISO:MGI.
DR InterPro; IPR020475; Endothelin.
DR InterPro; IPR019764; Endothelin_toxin_CS.
DR InterPro; IPR001928; Endothln-like_toxin.
DR PANTHER; PTHR13874; PTHR13874; 1.
DR Pfam; PF00322; Endothelin; 1.
DR PRINTS; PR00365; ENDOTHELIN.
DR SMART; SM00272; END; 2.
DR PROSITE; PS00270; ENDOTHELIN; 2.
PE 1: Evidence at protein level;
KW Cleavage on pair of basic residues; Direct protein sequencing;
KW Disulfide bond; Reference proteome; Secreted; Signal; Vasoactive;
KW Vasoconstrictor.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT PROPEP 26..50
FT /evidence="ECO:0000250|UniProtKB:P22388"
FT /id="PRO_0000008062"
FT PEPTIDE 53..91
FT /note="Big endothelin-1"
FT /evidence="ECO:0000250|UniProtKB:P22388"
FT /id="PRO_0000436397"
FT PEPTIDE 53..73
FT /note="Endothelin-1"
FT /id="PRO_0000008063"
FT PROPEP 74..202
FT /id="PRO_0000008064"
FT REGION 110..124
FT /note="Endothelin-like"
FT SITE 73..74
FT /note="Cleavage; by KEL"
FT /evidence="ECO:0000250|UniProtKB:P05305"
FT DISULFID 53..67
FT /evidence="ECO:0000250|UniProtKB:P05305"
FT DISULFID 55..63
FT /evidence="ECO:0000250|UniProtKB:P05305"
FT CONFLICT 75
FT /note="N -> K (in Ref. 3; BAA11109)"
FT /evidence="ECO:0000305"
FT CONFLICT 103
FT /note="A -> G (in Ref. 2; BAA07830)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 202 AA; 22809 MW; 3A0C1E1DFA797411 CRC64;
MDYFPVIFSL LFVTFQGAPE TAVLGAELST GAENGVQSPP PSTPWRPRRS KRCSCSSLMD
KECVYFCHLD IIWVNTPERV VPYGLGGSSR SKRSLKDLLP NKATDQAVRC QCAHQKDKKC
WNFCQAGKEL RAQSTMQKSL KDSKKGKPCS KLGKKCIYQQ LVEGRKLRRL EAISNSIKAS
FRVAKLKAEL YRDQKLTHNR AH
