ID   EDN2_CANLF              Reviewed;         178 AA.
AC   P12064; Q867C8;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 2.
DT   03-AUG-2022, entry version 134.
DE   RecName: Full=Endothelin-2;
DE            Short=ET-2;
DE   AltName: Full=Preproendothelin-2;
DE            Short=PPET2;
DE   Flags: Precursor;
GN   Name=EDN2;
OS   Canis lupus familiaris (Dog) (Canis familiaris).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Carnivora; Caniformia; Canidae; Canis.
OX   NCBI_TaxID=9615;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Uchide T.;
RT   "Cloning of dog preproendothelin-2 cDNA and quantitative method of the gene
RT   expression by real-time PCR.";
RL   Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 19-74.
RC   TISSUE=Kidney;
RX   PubMed=2668883; DOI=10.1093/nar/17.13.5389;
RA   Itoh Y., Kimura C., Onda H., Fujino M.;
RT   "Canine endothelin-2: cDNA sequence for the mature peptide.";
RL   Nucleic Acids Res. 17:5389-5389(1989).
CC   -!- FUNCTION: Endothelins are endothelium-derived vasoconstrictor peptides.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the endothelin/sarafotoxin family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA40354.1; Type=Miscellaneous discrepancy; Evidence={ECO:0000305};
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DR   EMBL; AB089264; BAC56713.1; -; mRNA.
DR   EMBL; X57038; CAA40354.1; ALT_SEQ; Genomic_DNA.
DR   PIR; S29563; S29563.
DR   RefSeq; NP_001003002.1; NM_001003002.1.
DR   AlphaFoldDB; P12064; -.
DR   STRING; 9612.ENSCAFP00000038061; -.
DR   PaxDb; P12064; -.
DR   Ensembl; ENSCAFT00030042218; ENSCAFP00030036831; ENSCAFG00030022935.
DR   Ensembl; ENSCAFT00040020170; ENSCAFP00040017495; ENSCAFG00040010846.
DR   Ensembl; ENSCAFT00845015151; ENSCAFP00845011762; ENSCAFG00845008596.
DR   GeneID; 403508; -.
DR   KEGG; cfa:403508; -.
DR   CTD; 1907; -.
DR   VEuPathDB; HostDB:ENSCAFG00845008596; -.
DR   eggNOG; ENOG502S5KM; Eukaryota.
DR   GeneTree; ENSGT00950000183053; -.
DR   InParanoid; P12064; -.
DR   OrthoDB; 1264335at2759; -.
DR   TreeFam; TF333184; -.
DR   Reactome; R-CFA-416476; G alpha (q) signalling events.
DR   Proteomes; UP000002254; Chromosome 15.
DR   Bgee; ENSCAFG00000002579; Expressed in hair follicle and 20 other tissues.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0031708; F:endothelin B receptor binding; IBA:GO_Central.
DR   GO; GO:0005179; F:hormone activity; IBA:GO_Central.
DR   GO; GO:0006874; P:cellular calcium ion homeostasis; IBA:GO_Central.
DR   GO; GO:0060585; P:positive regulation of prostaglandin-endoperoxide synthase activity; IBA:GO_Central.
DR   GO; GO:0003100; P:regulation of systemic arterial blood pressure by endothelin; IBA:GO_Central.
DR   GO; GO:0019229; P:regulation of vasoconstriction; IEA:InterPro.
DR   GO; GO:0014826; P:vein smooth muscle contraction; IBA:GO_Central.
DR   InterPro; IPR020475; Endothelin.
DR   InterPro; IPR019764; Endothelin_toxin_CS.
DR   InterPro; IPR001928; Endothln-like_toxin.
DR   InterPro; IPR015658; ET-2.
DR   PANTHER; PTHR13874; PTHR13874; 1.
DR   PANTHER; PTHR13874:SF9; PTHR13874:SF9; 1.
DR   Pfam; PF00322; Endothelin; 1.
DR   PRINTS; PR00365; ENDOTHELIN.
DR   SMART; SM00272; END; 2.
DR   PROSITE; PS00270; ENDOTHELIN; 2.
PE   2: Evidence at transcript level;
KW   Cleavage on pair of basic residues; Disulfide bond; Reference proteome;
KW   Secreted; Signal; Vasoactive; Vasoconstrictor.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   PROPEP          25..46
FT                   /id="PRO_0000008084"
FT   PEPTIDE         49..69
FT                   /note="Endothelin-2"
FT                   /id="PRO_0000008085"
FT   PROPEP          70..178
FT                   /id="PRO_0000008086"
FT   REGION          96..111
FT                   /note="Endothelin-like"
FT   REGION          156..178
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   SITE            69..70
FT                   /note="Cleavage; by KEL"
FT                   /evidence="ECO:0000250"
FT   DISULFID        49..63
FT                   /evidence="ECO:0000250"
FT   DISULFID        51..59
FT                   /evidence="ECO:0000250"
FT   CONFLICT        19..20
FT                   /note="VA -> AH (in Ref. 2; CAA40354)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   178 AA;  19723 MW;  5BE799BA3B9BB8AA CRC64;
     MPAPGVHHPN TASPFLKTVA AGKGQVAAAP EHPAPSARAR GSHLRPRRCS CSSWLDKECV
     YFCHLDIIWV NTPGQTAPYG LGNPPRRRRR SLPKRCECSS GGDPACATFC HRRPWAEAVV
     VPGSRSPADV FQAGRTWTSA GELLRQLRNI SAAKIRFPRR PQEAGRQLRP THPRRRKR