EDS1_YEAST
ID EDS1_YEAST Reviewed; 919 AA.
AC P38073; D6VQ33;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Transcriptional regulatory protein EDS1;
DE AltName: Full=Expression dependent on SLT2 protein 1;
GN Name=EDS1; OrderedLocusNames=YBR033W; ORFNames=YBR0318;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091864; DOI=10.1002/yea.320100010;
RA Smits P.H.M., de Haan M., Maat C., Grivell L.A.;
RT "The complete sequence of a 33 kb fragment on the right arm of chromosome
RT II from Saccharomyces cerevisiae reveals 16 open reading frames, including
RT ten new open reading frames, five previously identified genes and a
RT homologue of the SCO1 gene.";
RL Yeast 10:S75-S80(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP DNA-BINDING.
RX PubMed=19111667; DOI=10.1016/j.molcel.2008.11.020;
RA Badis G., Chan E.T., van Bakel H., Pena-Castillo L., Tillo D., Tsui K.,
RA Carlson C.D., Gossett A.J., Hasinoff M.J., Warren C.L., Gebbia M.,
RA Talukder S., Yang A., Mnaimneh S., Terterov D., Coburn D., Li Yeo A.,
RA Yeo Z.X., Clarke N.D., Lieb J.D., Ansari A.Z., Nislow C., Hughes T.R.;
RT "A library of yeast transcription factor motifs reveals a widespread
RT function for Rsc3 in targeting nucleosome exclusion at promoters.";
RL Mol. Cell 32:878-887(2008).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- SUBUNIT: Binds DNA in a sequence-specific manner.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the EDS1/RGT1 family. {ECO:0000305}.
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DR EMBL; X76078; CAA53688.1; -; Genomic_DNA.
DR EMBL; Z35902; CAA84975.1; -; Genomic_DNA.
DR EMBL; BK006936; DAA07153.1; -; Genomic_DNA.
DR PIR; S45889; S45889.
DR RefSeq; NP_009589.1; NM_001178381.1.
DR AlphaFoldDB; P38073; -.
DR BioGRID; 32734; 117.
DR DIP; DIP-4895N; -.
DR IntAct; P38073; 1.
DR STRING; 4932.YBR033W; -.
DR iPTMnet; P38073; -.
DR PaxDb; P38073; -.
DR PRIDE; P38073; -.
DR EnsemblFungi; YBR033W_mRNA; YBR033W; YBR033W.
DR GeneID; 852321; -.
DR KEGG; sce:YBR033W; -.
DR SGD; S000000237; EDS1.
DR VEuPathDB; FungiDB:YBR033W; -.
DR eggNOG; ENOG502QRVJ; Eukaryota.
DR GeneTree; ENSGT00940000176717; -.
DR HOGENOM; CLU_006525_0_0_1; -.
DR InParanoid; P38073; -.
DR OMA; HILENCC; -.
DR BioCyc; YEAST:G3O-29010-MON; -.
DR PRO; PR:P38073; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P38073; protein.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; HDA:SGD.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR CDD; cd00067; GAL4; 1.
DR Gene3D; 4.10.240.10; -; 1.
DR InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR Pfam; PF00172; Zn_clus; 1.
DR SMART; SM00066; GAL4; 1.
DR SUPFAM; SSF57701; SSF57701; 1.
DR PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Zinc.
FT CHAIN 1..919
FT /note="Transcriptional regulatory protein EDS1"
FT /id="PRO_0000114991"
FT DNA_BIND 56..85
FT /note="Zn(2)-C6 fungal-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT REGION 1..54
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 297..338
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 25..39
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 319..338
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 919 AA; 103397 MW; 18DD2E37A042E246 CRC64;
MSHHVPNLYG TPIRDPHERK RNSASMGEVN QSVSSRNCER GSEKGTKQRK KASHACDQCR
RKRIKCRFDK HTGVCQGCLE VGEKCQFIRV PLKRGPAKKR GSVVSIEKFS SDNDPLQYRP
RTHSYPMNSG NNYLPSLARN SSFPSISSLF VPSITAQSQQ FVKVPYDDIK RRSSLAILGS
DSSISTEFGG NYRLDENLNV RQEGKDIVAK GMITPVEEMG ACSSNVRRQG SQSLPIQEQR
ASPYINPFIS GRSRLSSLSY TSEATTSEGN TQGKNQCMLT PNSVRSIEKE RLNSLTAGFP
NKKLGTDGRS DKWDKNSTWK PVYRSSNPSH PSTEKNVSLN QEASAKPLML GTYRQFDATS
FYKVLGIYYN FFHINFPVIP INKSKFTDML DPEKPNVIDE IRQINNEIIQ CFKTALEVLV
FCKIKQRRSS KSTKSWSRDS LCDFQKGLYY IQNFNKCIAD CFQSLITIKP VLKQNSSVIP
SRIKFIYFST IIVLNFILIL AGEESSLLLG PSVGVFNEFQ AHKLFLPFQN TSPMLLLNSN
EESGDEILDY AVLFKRLYIL LNILDTLQSF RLGQPKLINL NFGSAIETYF SDKTGHNQVV
EKAPVALDNI LRNLKLGEFI TYFVLNRKSL QVNVPHHLLF TNQTDYGEFA VEKGEHDNIA
GKFETLLKKK EILIRKLLNI EQKNDHILEN CCNSDAEMKN IGELVCSMIT LVSGILDSIT
NMNAENSVDL DSKPLPNAYF AQDSEEELMS PTQSITSNLA SEENTRCTTK DLMGTVSIFM
LPMVEECYNI ISLIGPIPTT LISLYIRNGN MAKGINDRIM TLSTALNELV QITALFNTLE
PFRKNAHDRA KRYYVSATSS TGCYESVMKS MYSGKCAASN ASNVAPSEEE NKKILKKFAD
IGWKLMDDSE LGCCCCFFN
