EEN_ARATH
ID EEN_ARATH Reviewed; 124 AA.
AC Q8RWS0;
DT 03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Protein EIN6 ENHANCER {ECO:0000303|PubMed:31418686};
DE AltName: Full=Protein ENHANCER OF ETHYLENE INSENSITIVITY 6 {ECO:0000303|PubMed:31418686};
GN Name=EEN {ECO:0000303|PubMed:31418686};
GN OrderedLocusNames=At4g38495 {ECO:0000312|Araport:AT4G38495};
GN ORFNames=F20M13 {ECO:0000312|EMBL:AL035540},
GN F22I13 {ECO:0000312|EMBL:AL035539};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP AND INTERACTION WITH ARP5.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta;
RX PubMed=31418686; DOI=10.7554/elife.47835;
RA Zander M., Willige B.C., He Y., Nguyen T.A., Langford A.E., Nehring R.,
RA Howell E., McGrath R., Bartlett A., Castanon R., Nery J.R., Chen H.,
RA Zhang Z., Jupe F., Stepanova A., Schmitz R.J., Lewsey M.G., Chory J.,
RA Ecker J.R.;
RT "Epigenetic silencing of a multifunctional plant stress regulator.";
RL Elife 8:0-0(2019).
CC -!- FUNCTION: Proposed core component of the chromatin remodeling INO80
CC complex which is involved in transcriptional regulation, DNA
CC replication and probably DNA repair (By similarity). Together with
CC REF6/EIN6, involved in the epigenetic chromatin-dependent regulatory
CC mechanism that monitors the expression of the essential multifunctional
CC plant stress regulator EIN2 via H3K27me3 repressive histone
CC demethylation and histone variant H2A.Z eviction, thus modulating
CC responses to ethylene (ET), especially during embryogenesis
CC (PubMed:31418686). The INO80 complex controls ethylene-induced H2A.Z
CC eviction dynamics (PubMed:31418686). {ECO:0000250|UniProtKB:Q6PI98,
CC ECO:0000269|PubMed:31418686}.
CC -!- SUBUNIT: Component of the chromatin remodeling INO80 complex;
CC specifically part of a complex module associated with the helicase ATP-
CC binding and the helicase C-terminal domain of INO80 (PubMed:31418686).
CC Interacts with ARP5 (PubMed:31418686). {ECO:0000269|PubMed:31418686}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:31418686}. Cytoplasm
CC {ECO:0000269|PubMed:31418686}.
CC -!- TISSUE SPECIFICITY: Expressed ubiquitously in seedlings.
CC {ECO:0000269|PubMed:31418686}.
CC -!- DISRUPTION PHENOTYPE: Differential expression of several genes
CC (PubMed:31418686). Compromised ethylene-induced H2A.Z eviction dynamics
CC (PubMed:31418686). Plants missing both EEN and REF6/EIN6 (e.g. ref6-1
CC een-2 and ein6-1 een-1), are insensitive to ethylene (ET) and exhibit
CC reduced levels of EIN2 associated with a shift of the chromatin
CC landscape to a repressive state at its locus (e.g. H3K27me3 and H2A.Z)
CC (PubMed:31418686). {ECO:0000269|PubMed:31418686}.
CC -!- SIMILARITY: Belongs to the IES6 family. {ECO:0000305}.
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DR EMBL; AL035539; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL035540; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002687; AEE86934.1; -; Genomic_DNA.
DR EMBL; AY091150; AAM14098.1; -; mRNA.
DR EMBL; AY123018; AAM67551.1; -; mRNA.
DR EMBL; AK118900; BAC43484.1; -; mRNA.
DR RefSeq; NP_849518.1; NM_179187.1.
DR SMR; Q8RWS0; -.
DR STRING; 3702.AT4G38495.1; -.
DR PRIDE; Q8RWS0; -.
DR ProteomicsDB; 175650; -.
DR EnsemblPlants; AT4G38495.1; AT4G38495.1; AT4G38495.
DR GeneID; 830006; -.
DR Gramene; AT4G38495.1; AT4G38495.1; AT4G38495.
DR KEGG; ath:AT4G38495; -.
DR Araport; AT4G38495; -.
DR TAIR; locus:1005716392; AT4G38495.
DR eggNOG; KOG4137; Eukaryota.
DR HOGENOM; CLU_071116_3_0_1; -.
DR OMA; YVNIETP; -.
DR OrthoDB; 1560268at2759; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q8RWS0; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0031011; C:Ino80 complex; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0006338; P:chromatin remodeling; IMP:TAIR.
DR GO; GO:1902465; P:negative regulation of histone H3-K27 trimethylation; IMP:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:UniProtKB.
DR GO; GO:0010104; P:regulation of ethylene-activated signaling pathway; IMP:UniProtKB.
DR GO; GO:0040029; P:regulation of gene expression, epigenetic; IMP:UniProtKB.
DR InterPro; IPR029525; INO80C/Ies6.
DR InterPro; IPR013272; Vps72/YL1_C.
DR PANTHER; PTHR31200; PTHR31200; 1.
DR Pfam; PF08265; YL1_C; 1.
DR SMART; SM00993; YL1_C; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA damage; DNA recombination; DNA repair;
KW Ethylene signaling pathway; Nucleus; Reference proteome; Stress response;
KW Transcription; Transcription regulation.
FT CHAIN 1..124
FT /note="Protein EIN6 ENHANCER"
FT /id="PRO_0000456198"
SQ SEQUENCE 124 AA; 14322 MW; 058E24CC30C3B7D9 CRC64;
MERELMEADI VVASRLSFKK KQAYEKYPKA QSSSRRWKHL KQILQAENFP DLPPDLPTYV
NIESPPSTQP CKRLCDITGY EAPYVDPRTN LRYANAHVFQ TVRSLSSDQV HQYLSIRNAA
VVLK
