EER5_ARATH
ID EER5_ARATH Reviewed; 413 AA.
AC Q8GWE6; Q9ZUN6;
DT 20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Enhanced ethylene response protein 5 {ECO:0000303|PubMed:18429939};
DE AltName: Full=Nuclear mRNA export protein THP1 {ECO:0000303|PubMed:19843313};
DE Short=AtTHP1 {ECO:0000303|PubMed:19843313};
GN Name=EER5 {ECO:0000303|PubMed:18429939};
GN Synonyms=ESSP1 {ECO:0000303|PubMed:19843313},
GN THP1 {ECO:0000303|PubMed:19843313};
GN OrderedLocusNames=At2g19560 {ECO:0000312|Araport:AT2G19560};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:BAC43481.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP FUNCTION, MUTAGENESIS OF GLY-152, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE,
RP AND INTERACTION WITH EIN2 AND CSN8.
RX PubMed=18429939; DOI=10.1111/j.1365-313x.2008.03521.x;
RA Christians M.J., Robles L.M., Zeller S.M., Larsen P.B.;
RT "The eer5 mutation, which affects a novel proteasome-related subunit,
RT indicates a prominent role for the COP9 signalosome in resetting the
RT ethylene-signaling pathway in Arabidopsis.";
RL Plant J. 55:467-477(2008).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION,
RP AND INTERACTION WITH NUP1; DSS1(V); AMPD; SAC3A; SAC3B; UCH1 AND AT5G61290.
RX PubMed=19843313; DOI=10.1111/j.1365-313x.2009.04048.x;
RA Lu Q., Tang X., Tian G., Wang F., Liu K., Nguyen V., Kohalmi S.E.,
RA Keller W.A., Tsang E.W., Harada J.J., Rothstein S.J., Cui Y.;
RT "Arabidopsis homolog of the yeast TREX-2 mRNA export complex: components
RT and anchoring nucleoporin.";
RL Plant J. 61:259-270(2010).
RN [6]
RP INTERACTION WITH UCH1 AND UCH2.
RX PubMed=22951400; DOI=10.4161/psb.21899;
RA Tian G., Lu Q., Kohalmi S.E., Rothstein S.J., Cui Y.;
RT "Evidence that the Arabidopsis Ubiquitin C-terminal Hydrolases 1 and 2
RT associate with the 26S proteasome and the TREX-2 complex.";
RL Plant Signal. Behav. 7:1415-1419(2012).
CC -!- FUNCTION: Involved in the regulation of ethylene response
CC (PubMed:18429939). Probable TREX-2 component required for nuclear RNA
CC export. The TREX-2 complex (transcription and export complex 2)
CC functions in docking export-competent ribonucleoprotein particles
CC (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear
CC basket). TREX-2 participates in mRNA export and accurate chromatin
CC positioning in the nucleus by tethering genes to the nuclear periphery
CC (PubMed:19843313). {ECO:0000269|PubMed:18429939,
CC ECO:0000269|PubMed:19843313}.
CC -!- SUBUNIT: Interacts with EIN2 (via C-terminus). May also interact weakly
CC with CSN8 (PubMed:18429939). Interacts with DSS1(V), AMPD, SAC3A, SAC3B
CC and At5g61290 (AC Q9FLK4) (PubMed:19843313). Interacts with UCH1
CC (PubMed:19843313, PubMed:22951400) and UCH2 (PubMed:22951400).
CC Interacts with NUP1, anchoring the TREX-2 complex on the nuclear pore
CC complex (PubMed:19843313). {ECO:0000269|PubMed:18429939,
CC ECO:0000269|PubMed:19843313, ECO:0000269|PubMed:22951400}.
CC -!- INTERACTION:
CC Q8GWE6; Q9CAF4: NUP1; NbExp=3; IntAct=EBI-2619706, EBI-2619711;
CC Q8GWE6; F4IUY8: SAC3A; NbExp=3; IntAct=EBI-2619706, EBI-2619722;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:19843313}.
CC -!- TISSUE SPECIFICITY: Expressed at low levels in roots, leaves, stems and
CC shoots (PubMed:18429939). Detected in seedlings, roots, leaves and
CC anthers (PubMed:19843313). {ECO:0000269|PubMed:18429939,
CC ECO:0000269|PubMed:19843313}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype, when grown in absence of
CC ethylene. Extreme ethylene responsivness, when treated with saturating
CC ethylene concentrations (PubMed:18429939). Curly leaf
CC (PubMed:19843313). {ECO:0000269|PubMed:18429939,
CC ECO:0000269|PubMed:19843313}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD10155.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC005917; AAD10155.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC06895.1; -; Genomic_DNA.
DR EMBL; AK118897; BAC43481.1; -; mRNA.
DR PIR; C84578; C84578.
DR RefSeq; NP_179546.2; NM_127514.4.
DR AlphaFoldDB; Q8GWE6; -.
DR SMR; Q8GWE6; -.
DR IntAct; Q8GWE6; 7.
DR STRING; 3702.AT2G19560.1; -.
DR iPTMnet; Q8GWE6; -.
DR PaxDb; Q8GWE6; -.
DR PRIDE; Q8GWE6; -.
DR ProteomicsDB; 224727; -.
DR DNASU; 816475; -.
DR EnsemblPlants; AT2G19560.1; AT2G19560.1; AT2G19560.
DR GeneID; 816475; -.
DR Gramene; AT2G19560.1; AT2G19560.1; AT2G19560.
DR KEGG; ath:AT2G19560; -.
DR Araport; AT2G19560; -.
DR TAIR; locus:2050414; AT2G19560.
DR eggNOG; KOG2688; Eukaryota.
DR HOGENOM; CLU_031567_2_0_1; -.
DR InParanoid; Q8GWE6; -.
DR OrthoDB; 706700at2759; -.
DR PhylomeDB; Q8GWE6; -.
DR PRO; PR:Q8GWE6; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8GWE6; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0070390; C:transcription export complex 2; IBA:GO_Central.
DR GO; GO:0003690; F:double-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0009873; P:ethylene-activated signaling pathway; IPI:TAIR.
DR GO; GO:0016973; P:poly(A)+ mRNA export from nucleus; IMP:TAIR.
DR GO; GO:0000973; P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery; IBA:GO_Central.
DR GO; GO:0009723; P:response to ethylene; IMP:TAIR.
DR GO; GO:0048364; P:root development; IMP:TAIR.
DR GO; GO:0006368; P:transcription elongation from RNA polymerase II promoter; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR045114; Csn12-like.
DR InterPro; IPR000717; PCI_dom.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR12732; PTHR12732; 1.
DR Pfam; PF01399; PCI; 1.
DR PROSITE; PS50250; PCI; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome.
FT CHAIN 1..413
FT /note="Enhanced ethylene response protein 5"
FT /id="PRO_0000435316"
FT DOMAIN 216..402
FT /note="PCI"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01185"
FT MUTAGEN 152
FT /note="G->E: In eer5-1; enhanced ethylene response."
FT /evidence="ECO:0000269|PubMed:18429939"
SQ SEQUENCE 413 AA; 47614 MW; 9D60990CAF1C3A76 CRC64;
MAYVSMGEAH RRITEYLNRF CDAVSYQDSS TLCRLLSFSS NSPPLLSLAD ALNVFQDSSS
LIRQSDRFSE YGEILAHVFR SLQSYRVGNL VEAYLAFDKF ANAFVQEFRN WESAWALEAL
YVVCYEIRVL AEKADKDLTS NGKSPEKLKA AGSLLMKVFG VLAGKGPKRV GALYVTCQLF
KTYFKLGTVN LCRSVIRSIE TARIFDFEEF PRRDKVTYMY YTGRLEVFNE NFPAADTKLS
YALQNCNPKR ERNIRMILKY LVPVKLSLGI IPKDELLRNY NLHEYTKIVQ ALRKGDLRLL
RHALQEHEDR FLRSGVYLVL EKLELQVYQR LMKKIYINQK LSDPARAHQL KLEGIAKALR
WLDMDMDLDE VECIMTILIY KNLVKGYLAH KSKVVVLSKQ DPFPKLNGKP VSS
