EF101_ARATH
ID EF101_ARATH Reviewed; 243 AA.
AC O80338; Q682Y8; Q8LBU9;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Ethylene-responsive transcription factor 2;
DE Short=AtERF2;
DE AltName: Full=Ethylene-responsive element-binding factor 2;
DE Short=EREBP-2;
GN Name=ERF2; Synonyms=ERF-2, ERF101; OrderedLocusNames=At5g47220;
GN ORFNames=MQL5.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND INDUCTION.
RX PubMed=10715325; DOI=10.2307/3870944;
RA Fujimoto S.Y., Ohta M., Usui A., Shinshi H., Ohme-Takagi M.;
RT "Arabidopsis ethylene responsive element binding factors act as
RT transcriptional activators or repressors of GCC box mediated gene
RT expression.";
RL Plant Cell 12:393-404(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP FUNCTION.
RX PubMed=9756931; DOI=10.1074/jbc.273.41.26857;
RA Hao D., Ohme-Takagi M., Sarai A.;
RT "Unique mode of GCC box recognition by the DNA-binding domain of ethylene-
RT responsive element-binding factor (ERF domain) in plant.";
RL J. Biol. Chem. 273:26857-26861(1998).
RN [8]
RP FUNCTION, AND INDUCTION.
RX PubMed=12805630; DOI=10.1104/pp.102.017814;
RA Brown R.L., Kazan K., McGrath K.C., Maclean D.J., Manners J.M.;
RT "A role for the GCC-box in jasmonate-mediated activation of the PDF1.2 gene
RT of Arabidopsis.";
RL Plant Physiol. 132:1020-1032(2003).
RN [9]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16407444; DOI=10.1104/pp.105.073783;
RA Nakano T., Suzuki K., Fujimura T., Shinshi H.;
RT "Genome-wide analysis of the ERF gene family in Arabidopsis and rice.";
RL Plant Physiol. 140:411-432(2006).
CC -!- FUNCTION: Acts as a transcriptional activator. Binds to the GCC-box
CC pathogenesis-related promoter element. Involved in the regulation of
CC gene expression by stress factors and by components of stress signal
CC transduction pathways. Involved in disease resistance pathways.
CC {ECO:0000269|PubMed:10715325, ECO:0000269|PubMed:12805630,
CC ECO:0000269|PubMed:9756931}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- INDUCTION: Induced by jasmonate (JA) and Alternaria brassicicola
CC (locally and systemically). Ethylene induction is completely dependent
CC on a functional ETHYLENE-INSENSITIVE2 (EIN2) while wounding induction
CC does not require EIN2. Transcripts accumulate strongly in
CC cycloheximide-treated plants, a protein synthesis inhibitor. Seems to
CC not be influenced by exogenous abscisic acid (ABA), cold, heat, NaCl or
CC drought stress. {ECO:0000269|PubMed:10715325,
CC ECO:0000269|PubMed:12805630}.
CC -!- DOMAIN: The AP2/ERF domain binds specifically to the 5'-GCCGCC-3'
CC motif. The affinity of this binding is higher if the seventh amino-acid
CC of this domain is basic (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the AP2/ERF transcription factor family. ERF
CC subfamily. {ECO:0000305}.
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DR EMBL; AB008104; BAA32419.1; -; mRNA.
DR EMBL; AB018117; BAA97155.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95487.1; -; Genomic_DNA.
DR EMBL; AK175151; BAD42914.1; -; mRNA.
DR EMBL; AK175229; BAD42992.1; -; mRNA.
DR EMBL; AK176532; BAD44295.1; -; mRNA.
DR EMBL; AK176606; BAD44369.1; -; mRNA.
DR EMBL; AK176825; BAD44588.1; -; mRNA.
DR EMBL; BT024470; ABD19651.1; -; mRNA.
DR EMBL; AY086983; AAM64544.1; -; mRNA.
DR PIR; T51989; T51989.
DR RefSeq; NP_199533.1; NM_124093.3.
DR AlphaFoldDB; O80338; -.
DR SMR; O80338; -.
DR BioGRID; 20016; 11.
DR IntAct; O80338; 6.
DR STRING; 3702.AT5G47220.1; -.
DR PaxDb; O80338; -.
DR PRIDE; O80338; -.
DR EnsemblPlants; AT5G47220.1; AT5G47220.1; AT5G47220.
DR GeneID; 834768; -.
DR Gramene; AT5G47220.1; AT5G47220.1; AT5G47220.
DR KEGG; ath:AT5G47220; -.
DR Araport; AT5G47220; -.
DR TAIR; locus:2171514; AT5G47220.
DR eggNOG; ENOG502QRIC; Eukaryota.
DR HOGENOM; CLU_058713_3_0_1; -.
DR InParanoid; O80338; -.
DR OMA; LSCITEF; -.
DR OrthoDB; 1213926at2759; -.
DR PhylomeDB; O80338; -.
DR PRO; PR:O80338; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; O80338; baseline and differential.
DR Genevisible; O80338; AT.
DR GO; GO:0005634; C:nucleus; TAS:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0051301; P:cell division; IEP:TAIR.
DR GO; GO:0009873; P:ethylene-activated signaling pathway; TAS:TAIR.
DR GO; GO:0009864; P:induced systemic resistance, jasmonic acid mediated signaling pathway; IMP:TAIR.
DR GO; GO:0010087; P:phloem or xylem histogenesis; IEP:TAIR.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:TAIR.
DR CDD; cd00018; AP2; 1.
DR Gene3D; 3.30.730.10; -; 1.
DR InterPro; IPR001471; AP2/ERF_dom.
DR InterPro; IPR036955; AP2/ERF_dom_sf.
DR InterPro; IPR016177; DNA-bd_dom_sf.
DR InterPro; IPR044808; ERF_plant.
DR PANTHER; PTHR31190; PTHR31190; 1.
DR Pfam; PF00847; AP2; 1.
DR PRINTS; PR00367; ETHRSPELEMNT.
DR SMART; SM00380; AP2; 1.
DR SUPFAM; SSF54171; SSF54171; 1.
DR PROSITE; PS51032; AP2_ERF; 1.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Ethylene signaling pathway; Nucleus; Plant defense;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..243
FT /note="Ethylene-responsive transcription factor 2"
FT /id="PRO_0000112546"
FT DNA_BIND 116..174
FT /note="AP2/ERF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00366"
FT REGION 181..219
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 185..207
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 168
FT /note="R -> S (in Ref. 6; AAM64544)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 243 AA; 26797 MW; C9A4C9791249B5D4 CRC64;
MYGQCNIESD YALLESITRH LLGGGGENEL RLNESTPSSC FTESWGGLPL KENDSEDMLV
YGLLKDAFHF DTSSSDLSCL FDFPAVKVEP TENFTAMEEK PKKAIPVTET AVKAKHYRGV
RQRPWGKFAA EIRDPAKNGA RVWLGTFETA EDAALAYDIA AFRMRGSRAL LNFPLRVNSG
EPDPVRITSK RSSSSSSSSS SSTSSSENGK LKRRRKAENL TSEVVQVKCE VGDETRVDEL
LVS
