EF102_ARATH
ID EF102_ARATH Reviewed; 300 AA.
AC O80341; Q9LVT6;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Ethylene-responsive transcription factor 5;
DE Short=AtERF5;
DE AltName: Full=Ethylene-responsive element-binding factor 5;
DE Short=EREBP-5;
GN Name=ERF5; Synonyms=ERF-5, ERF102; OrderedLocusNames=At5g47230;
GN ORFNames=MQL5_9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND INDUCTION.
RX PubMed=10715325; DOI=10.2307/3870944;
RA Fujimoto S.Y., Ohta M., Usui A., Shinshi H., Ohme-Takagi M.;
RT "Arabidopsis ethylene responsive element binding factors act as
RT transcriptional activators or repressors of GCC box mediated gene
RT expression.";
RL Plant Cell 12:393-404(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP FUNCTION.
RX PubMed=9756931; DOI=10.1074/jbc.273.41.26857;
RA Hao D., Ohme-Takagi M., Sarai A.;
RT "Unique mode of GCC box recognition by the DNA-binding domain of ethylene-
RT responsive element-binding factor (ERF domain) in plant.";
RL J. Biol. Chem. 273:26857-26861(1998).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16407444; DOI=10.1104/pp.105.073783;
RA Nakano T., Suzuki K., Fujimura T., Shinshi H.;
RT "Genome-wide analysis of the ERF gene family in Arabidopsis and rice.";
RL Plant Physiol. 140:411-432(2006).
CC -!- FUNCTION: Acts as a transcriptional activator. Binds to the GCC-box
CC pathogenesis-related promoter element. Involved in the regulation of
CC gene expression by stress factors and by components of stress signal
CC transduction pathways. {ECO:0000269|PubMed:10715325,
CC ECO:0000269|PubMed:9756931}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- INDUCTION: Ethylene induction is completely dependent on a functional
CC ETHYLENE-INSENSITIVE2 (EIN2). Wounding as well as cold stress induction
CC does not require EIN2. Transcripts accumulate strongly in
CC cycloheximide-treated plants, a protein synthesis inhibitor. Seems to
CC not be influenced by jasmonate, Alternaria brassicicola, exogenous
CC abscisic acid (ABA), cold, heat, NaCl or drought stress.
CC {ECO:0000269|PubMed:10715325}.
CC -!- DOMAIN: The AP2/ERF domain binds specifically to the 5'-GCCGCC-3'
CC motif. The affinity of this binding is higher if the seventh amino-acid
CC of this domain is basic (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the AP2/ERF transcription factor family. ERF
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA97157.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB008107; BAA32422.1; -; mRNA.
DR EMBL; AB018117; BAA97157.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED95489.1; -; Genomic_DNA.
DR EMBL; AK117568; BAC42229.1; -; mRNA.
DR EMBL; AF385709; AAK60301.1; -; mRNA.
DR EMBL; AY078014; AAL77715.1; -; mRNA.
DR PIR; T52020; T52020.
DR RefSeq; NP_568679.1; NM_124094.3.
DR AlphaFoldDB; O80341; -.
DR SMR; O80341; -.
DR BioGRID; 20018; 6.
DR IntAct; O80341; 3.
DR STRING; 3702.AT5G47230.1; -.
DR PaxDb; O80341; -.
DR PRIDE; O80341; -.
DR EnsemblPlants; AT5G47230.1; AT5G47230.1; AT5G47230.
DR GeneID; 834770; -.
DR Gramene; AT5G47230.1; AT5G47230.1; AT5G47230.
DR KEGG; ath:AT5G47230; -.
DR Araport; AT5G47230; -.
DR TAIR; locus:2171529; AT5G47230.
DR eggNOG; ENOG502RXE3; Eukaryota.
DR HOGENOM; CLU_058713_0_1_1; -.
DR InParanoid; O80341; -.
DR OMA; PIDCSES; -.
DR OrthoDB; 1169658at2759; -.
DR PhylomeDB; O80341; -.
DR PRO; PR:O80341; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; O80341; baseline and differential.
DR Genevisible; O80341; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0009873; P:ethylene-activated signaling pathway; TAS:TAIR.
DR GO; GO:0009733; P:response to auxin; IEP:TAIR.
DR GO; GO:0009409; P:response to cold; IEP:TAIR.
DR CDD; cd00018; AP2; 1.
DR Gene3D; 3.30.730.10; -; 1.
DR InterPro; IPR001471; AP2/ERF_dom.
DR InterPro; IPR036955; AP2/ERF_dom_sf.
DR InterPro; IPR016177; DNA-bd_dom_sf.
DR Pfam; PF00847; AP2; 1.
DR PRINTS; PR00367; ETHRSPELEMNT.
DR SMART; SM00380; AP2; 1.
DR SUPFAM; SSF54171; SSF54171; 1.
DR PROSITE; PS51032; AP2_ERF; 1.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Ethylene signaling pathway; Nucleus; Plant defense;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..300
FT /note="Ethylene-responsive transcription factor 5"
FT /id="PRO_0000112563"
FT DNA_BIND 155..213
FT /note="AP2/ERF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00366"
FT REGION 28..48
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 29..48
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 300 AA; 33810 MW; 1189D464A28F7251 CRC64;
MATPNEVSAL WFIEKHLLDE ASPVATDPWM KHESSSATES SSDSSSIIFG SSSSSFAPID
FSESVCKPEI IDLDTPRSME FLSIPFEFDS EVSVSDFDFK PSNQNQNQFE PELKSQIRKP
PLKISLPAKT EWIQFAAENT KPEVTKPVSE EEKKHYRGVR QRPWGKFAAE IRDPNKRGSR
VWLGTFDTAI EAARAYDEAA FRLRGSKAIL NFPLEVGKWK PRADEGEKKR KRDDDEKVTV
VEKVLKTEQS VDVNGGETFP FVTSNLTELC DWDLTGFLNF PLLSPLSPHP PFGYSQLTVV
