ID   EF1A1_EUPCR             Reviewed;         442 AA.
AC   Q27139;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Elongation factor 1-alpha 1;
DE            Short=EF-1-alpha-1;
GN   Name=EFA1;
OS   Euplotes crassus.
OC   Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata; Spirotrichea;
OC   Hypotrichia; Euplotida; Euplotidae; Moneuplotes.
OX   NCBI_TaxID=5936;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=POR3;
RX   PubMed=8626055; DOI=10.1016/0378-1119(95)00728-8;
RA   Bergemann J., Florian V., Kremser T., Klein A.;
RT   "Two different macronuclear EF-1 alpha-encoding genes of the ciliate
RT   Euplotes crassus are very dissimilar in their sequences, copy numbers and
RT   transcriptional activities.";
RL   Gene 168:109-112(1996).
CC   -!- FUNCTION: This protein promotes the GTP-dependent binding of aminoacyl-
CC       tRNA to the A-site of ribosomes during protein biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC       superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A
CC       subfamily. {ECO:0000305}.
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DR   EMBL; U26260; AAB04943.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q27139; -.
DR   SMR; Q27139; -.
DR   PRIDE; Q27139; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00118_A; EF_Tu_A; 1.
DR   InterPro; IPR004161; EFTu-like_2.
DR   InterPro; IPR031157; G_TR_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR   InterPro; IPR009000; Transl_B-barrel_sf.
DR   InterPro; IPR009001; Transl_elong_EF1A/Init_IF2_C.
DR   InterPro; IPR004539; Transl_elong_EF1A_euk/arc.
DR   InterPro; IPR004160; Transl_elong_EFTu/EF1A_C.
DR   Pfam; PF00009; GTP_EFTU; 1.
DR   Pfam; PF03144; GTP_EFTU_D2; 1.
DR   Pfam; PF03143; GTP_EFTU_D3; 1.
DR   PRINTS; PR00315; ELONGATNFCT.
DR   SUPFAM; SSF50447; SSF50447; 1.
DR   SUPFAM; SSF50465; SSF50465; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00483; EF-1_alpha; 1.
DR   PROSITE; PS00301; G_TR_1; 1.
DR   PROSITE; PS51722; G_TR_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Elongation factor; GTP-binding; Nucleotide-binding;
KW   Protein biosynthesis.
FT   CHAIN           1..442
FT                   /note="Elongation factor 1-alpha 1"
FT                   /id="PRO_0000090923"
FT   DOMAIN          5..227
FT                   /note="tr-type G"
FT   REGION          14..21
FT                   /note="G1"
FT                   /evidence="ECO:0000250"
FT   REGION          70..74
FT                   /note="G2"
FT                   /evidence="ECO:0000250"
FT   REGION          91..94
FT                   /note="G3"
FT                   /evidence="ECO:0000250"
FT   REGION          153..156
FT                   /note="G4"
FT                   /evidence="ECO:0000250"
FT   REGION          194..196
FT                   /note="G5"
FT                   /evidence="ECO:0000250"
FT   BINDING         14..21
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         91..95
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         153..156
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   442 AA;  48537 MW;  423E15FB3292D265 CRC64;
     MGKEKEHLNL VVIGHVDSGK STTTGHLIYK LGGIDARTIE KFEKESAEMG KASFKYAWVL
     DKLKAERERG ITIDIALWKF ETENRHYTII DAPGHRDFIK NMITGTSQAD AAILIIASGT
     GEFEAGISKE GQTREHALLA YTMGVKQMVV AMNKMDSTEP PYSEDRYEEI KKEVSTFLAK
     VGYKPAKMNF VPISGFQGDN IQENSTNMPW YKGPTLCAAL DSFKIPKRPI AKPLRLPLQD
     VYKIGGIGTV PVGRVETGVL KAGMVITFAP KGCSAECKSV EMHHEEVPEA APGNNVGFNV
     KGLSVKDIKR GFVASDSKND PATDTESFVS HTIVMNHPGE IKAGYTPVID CHTAHIACKF
     EELLTKADKR SGKMTEENPK FLKAGDAGLI RLSPSKPLCV ETFATYAPLG RFAVRDMRQT
     VAVGVIQEIK KKATEDKKGK KK