EF1A_ORYSJ
ID EF1A_ORYSJ Reviewed; 447 AA.
AC O64937; A3AEP9; O49831; O50075; Q10QZ6;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2006, sequence version 2.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Elongation factor 1-alpha;
DE Short=EF-1-alpha;
GN Name=REFA1;
GN OrderedLocusNames=Os03g0177400 {ECO:0000312|EMBL:BAS82584.1},
GN LOC_Os03g08010 {ECO:0000305};
GN and
GN Name=REFA2;
GN OrderedLocusNames=Os03g0178000 {ECO:0000312|EMBL:BAS82591.1},
GN LOC_Os03g08060 {ECO:0000305};
GN and
GN Name=REFA3;
GN OrderedLocusNames=Os03g0177900 {ECO:0000312|EMBL:BAS82588.1},
GN LOC_Os03g08050 {ECO:0000305};
GN and
GN Name=REFA4;
GN OrderedLocusNames=Os03g0177500 {ECO:0000312|EMBL:BAS82586.1},
GN LOC_Os03g08020 {ECO:0000305};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (REFA1; REFA2; REFA3 AND REFA4).
RC STRAIN=cv. Hayayuki;
RX PubMed=9484469; DOI=10.1023/a:1005960721762;
RA Kidou S., Ejiri S.;
RT "Isolation, characterization and mRNA expression of four cDNAs encoding
RT translation elongation factor 1A from rice (Oryza sativa L.).";
RL Plant Mol. Biol. 36:137-148(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (REFA1).
RC STRAIN=cv. Tainung 67;
RA Lin T.-Y., Wang J.-F., Liu K.-H., Wang C.-T.;
RT "Cloning and sequencing of a rice cDNA encoding translation elongation
RT factor-1 alpha.";
RL (er) Plant Gene Register PGR98-058(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Ilpoombyeo; TISSUE=Seed;
RA Yoon U.H., Kim Y.H.;
RT "Structural and expression analysis of germinating seed genes in Oryza
RT sativa L.";
RL Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (REFA1; REFA2; REFA3 AND
RP REFA4).
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: This protein promotes the GTP-dependent binding of aminoacyl-
CC tRNA to the A-site of ribosomes during protein biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A
CC subfamily. {ECO:0000305}.
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DR EMBL; D63580; BAA23657.1; -; mRNA.
DR EMBL; D63581; BAA23658.1; -; mRNA.
DR EMBL; D63582; BAA23659.1; -; mRNA.
DR EMBL; D63583; BAA23660.1; -; mRNA.
DR EMBL; AF030517; AAC15413.1; -; mRNA.
DR EMBL; GQ848058; ADM86871.1; -; mRNA.
DR EMBL; GQ848074; ADM86887.1; -; mRNA.
DR EMBL; GQ848073; ADM86886.1; -; mRNA.
DR EMBL; GQ848072; ADM86885.1; -; mRNA.
DR EMBL; AC090484; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; DP000009; ABF94269.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF94270.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF94273.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF94274.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS82584.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS82591.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS82588.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS82586.1; -; Genomic_DNA.
DR EMBL; CM000140; EAZ25791.1; -; Genomic_DNA.
DR EMBL; CM000140; EAZ25792.1; -; Genomic_DNA.
DR EMBL; AK061464; BAG87945.1; -; mRNA.
DR EMBL; AK072648; BAG93078.1; -; mRNA.
DR EMBL; AK105030; BAG97075.1; -; mRNA.
DR RefSeq; XP_015629735.1; XM_015774249.1.
DR RefSeq; XP_015629737.1; XM_015774251.1.
DR RefSeq; XP_015629802.1; XM_015774316.1.
DR RefSeq; XP_015629803.1; XM_015774317.1.
DR AlphaFoldDB; O64937; -.
DR SMR; O64937; -.
DR STRING; 4530.OS03T0177400-01; -.
DR PaxDb; O64937; -.
DR PRIDE; O64937; -.
DR EnsemblPlants; Os03t0177400-01; Os03t0177400-01; Os03g0177400.
DR EnsemblPlants; Os03t0177500-01; Os03t0177500-01; Os03g0177500.
DR EnsemblPlants; Os03t0177900-01; Os03t0177900-01; Os03g0177900.
DR EnsemblPlants; Os03t0178000-01; Os03t0178000-01; Os03g0178000.
DR EnsemblPlants; Os03t0178000-02; Os03t0178000-02; Os03g0178000.
DR GeneID; 4331810; -.
DR GeneID; 4331811; -.
DR GeneID; 4331812; -.
DR GeneID; 4331813; -.
DR Gramene; Os03t0177400-01; Os03t0177400-01; Os03g0177400.
DR Gramene; Os03t0177500-01; Os03t0177500-01; Os03g0177500.
DR Gramene; Os03t0177900-01; Os03t0177900-01; Os03g0177900.
DR Gramene; Os03t0178000-01; Os03t0178000-01; Os03g0178000.
DR Gramene; Os03t0178000-02; Os03t0178000-02; Os03g0178000.
DR KEGG; osa:4331810; -.
DR KEGG; osa:4331811; -.
DR KEGG; osa:4331812; -.
DR KEGG; osa:4331813; -.
DR eggNOG; KOG0052; Eukaryota.
DR HOGENOM; CLU_007265_3_5_1; -.
DR InParanoid; O64937; -.
DR OMA; AIRDMGM; -.
DR OrthoDB; 1150082at2759; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000007752; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR ExpressionAtlas; O64937; baseline and differential.
DR Genevisible; O64937; OS.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0003746; F:translation elongation factor activity; IBA:GO_Central.
DR GO; GO:0006412; P:translation; IBA:GO_Central.
DR GO; GO:0006414; P:translational elongation; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00118_A; EF_Tu_A; 1.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR031157; G_TR_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR InterPro; IPR009001; Transl_elong_EF1A/Init_IF2_C.
DR InterPro; IPR004539; Transl_elong_EF1A_euk/arc.
DR InterPro; IPR004160; Transl_elong_EFTu/EF1A_C.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR Pfam; PF03143; GTP_EFTU_D3; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF50447; SSF50447; 1.
DR SUPFAM; SSF50465; SSF50465; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00483; EF-1_alpha; 1.
DR PROSITE; PS00301; G_TR_1; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Elongation factor; GTP-binding; Methylation; Nucleotide-binding;
KW Phosphoprotein; Protein biosynthesis; Reference proteome.
FT CHAIN 1..447
FT /note="Elongation factor 1-alpha"
FT /id="PRO_0000090941"
FT DOMAIN 5..230
FT /note="tr-type G"
FT REGION 14..21
FT /note="G1"
FT /evidence="ECO:0000250"
FT REGION 70..74
FT /note="G2"
FT /evidence="ECO:0000250"
FT REGION 91..94
FT /note="G3"
FT /evidence="ECO:0000250"
FT REGION 153..156
FT /note="G4"
FT /evidence="ECO:0000250"
FT REGION 194..196
FT /note="G5"
FT /evidence="ECO:0000250"
FT BINDING 14..21
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 91..95
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 153..156
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT MOD_RES 55
FT /note="N6,N6-dimethyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q8GTY0"
FT MOD_RES 79
FT /note="N6,N6,N6-trimethyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q8GTY0"
FT MOD_RES 187
FT /note="N6,N6,N6-trimethyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q8GTY0"
FT MOD_RES 261
FT /note="N6-methyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q8GTY0"
FT MOD_RES 289
FT /note="5-glutamyl glycerylphosphorylethanolamine"
FT /evidence="ECO:0000250"
FT MOD_RES 306
FT /note="N6,N6,N6-trimethyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q8GTY0"
FT MOD_RES 362
FT /note="5-glutamyl glycerylphosphorylethanolamine"
FT /evidence="ECO:0000250"
FT MOD_RES 396
FT /note="N6,N6,N6-trimethyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q8GTY0"
FT CONFLICT 227
FT /note="K -> N (in Ref. 1; BAA23658)"
FT /evidence="ECO:0000305"
FT CONFLICT 444
FT /note="A -> R (in Ref. 2; AAC15413)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 447 AA; 49293 MW; 7B8065A1D0520F3C CRC64;
MGKEKTHINI VVIGHVDSGK STTTGHLIYK LGGIDKRVIE RFEKEAAEMN KRSFKYAWVL
DKLKAERERG ITIDIALWKF ETTKYYCTVI DAPGHRDFIK NMITGTSQAD CAVLIIDSTT
GGFEAGISKD GQTREHALLA FTLGVKQMIC CCNKMDATTP KYSKARYDEI VKEVSSYLKK
VGYNPDKIPF VPISGFEGDN MIERSTNLDW YKGPTLLEAL DQINEPKRPS DKPLRLPLQD
VYKIGGIGTV PVGRVETGVL KPGMVVTFGP SGLTTEVKSV EMHHEALQEA LPGDNVGFNV
KNVAVKDLKR GYVASNSKDD PAKEAASFTS QVIIMNHPGQ IGNGYAPVLD CHTSHIAVKF
AELVTKIDRR SGKELEKEPK FLKNGDAGMV KMIPTKPMVV ETFSEYPPLG RFAVRDMRQT
VAVGVIKNVE KKDPTGAKVT KAAAKKK
