EF1A_SACS2
ID EF1A_SACS2 Reviewed; 435 AA.
AC P35021;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 3.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Elongation factor 1-alpha {ECO:0000255|HAMAP-Rule:MF_00118};
DE Short=EF-1-alpha {ECO:0000255|HAMAP-Rule:MF_00118};
DE AltName: Full=Elongation factor Tu {ECO:0000255|HAMAP-Rule:MF_00118};
DE Short=EF-Tu {ECO:0000255|HAMAP-Rule:MF_00118};
GN Name=tuf {ECO:0000255|HAMAP-Rule:MF_00118}; Synonyms=tef1;
GN OrderedLocusNames=SSO0216;
OS Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
OS (Sulfolobus solfataricus).
OC Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC Saccharolobus.
OX NCBI_TaxID=273057;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=DSM 5833 / MT-4;
RX PubMed=8369039; DOI=10.1093/nar/21.7.1666;
RA Arcari P., Gallo M., Ianniciello G., Dello Russo A., Bocchini V.;
RT "Primary structure of the elongation factor 1 alpha in Sulfolobus
RT solfataricus.";
RL Nucleic Acids Res. 21:1666-1666(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=DSM 5833 / MT-4;
RX PubMed=8148382; DOI=10.1016/0167-4781(94)90296-8;
RA Arcari P., Gallo M., Ianniciello G., Dello Russo A., Bocchini V.;
RT "The nucleotide sequence of the gene coding for the elongation factor 1
RT alpha in Sulfolobus solfataricus. Homology of the product with related
RT proteins.";
RL Biochim. Biophys. Acta 1217:333-337(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=MT-3;
RA Arcari P., Masullo M., Bocchini V.;
RT "The nucleotide sequence of the gene encoding the elongation factor 1 alpha
RT from the archaeon Sulfolobus solfataricus isolate MT3.";
RL Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX PubMed=11427726; DOI=10.1073/pnas.141222098;
RA She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J.,
RA Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G.,
RA Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J.,
RA Medina N., Peng X., Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C.,
RA Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T.,
RA Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.;
RT "The complete genome of the crenarchaeon Sulfolobus solfataricus P2.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001).
CC -!- FUNCTION: This protein promotes the GTP-dependent binding of aminoacyl-
CC tRNA to the A-site of ribosomes during protein biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00118}.
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DR EMBL; X70701; CAA50033.1; -; Genomic_DNA.
DR EMBL; X76767; CAA54162.1; -; Genomic_DNA.
DR EMBL; AJ312397; CAC42886.1; -; Genomic_DNA.
DR EMBL; AE006641; AAK40559.1; -; Genomic_DNA.
DR PIR; H90162; H90162.
DR PIR; S43507; S43507.
DR RefSeq; WP_010922916.1; NC_002754.1.
DR PDB; 1JNY; X-ray; 1.80 A; A/B=1-435.
DR PDB; 1SKQ; X-ray; 1.80 A; A/B=1-435.
DR PDBsum; 1JNY; -.
DR PDBsum; 1SKQ; -.
DR AlphaFoldDB; P35021; -.
DR SMR; P35021; -.
DR STRING; 273057.SSO0216; -.
DR PRIDE; P35021; -.
DR EnsemblBacteria; AAK40559; AAK40559; SSO0216.
DR GeneID; 27426513; -.
DR KEGG; sso:SSO0216; -.
DR PATRIC; fig|273057.12.peg.214; -.
DR eggNOG; arCOG01561; Archaea.
DR HOGENOM; CLU_007265_3_5_2; -.
DR InParanoid; P35021; -.
DR OMA; AIRDMGM; -.
DR PhylomeDB; P35021; -.
DR BRENDA; 3.6.5.3; 6163.
DR EvolutionaryTrace; P35021; -.
DR Proteomes; UP000001974; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00118_A; EF_Tu_A; 1.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR031157; G_TR_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR InterPro; IPR009001; Transl_elong_EF1A/Init_IF2_C.
DR InterPro; IPR004539; Transl_elong_EF1A_euk/arc.
DR InterPro; IPR004160; Transl_elong_EFTu/EF1A_C.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR Pfam; PF03143; GTP_EFTU_D3; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF50447; SSF50447; 1.
DR SUPFAM; SSF50465; SSF50465; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00483; EF-1_alpha; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS00301; G_TR_1; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Elongation factor; GTP-binding;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1..435
FT /note="Elongation factor 1-alpha"
FT /id="PRO_0000090994"
FT DOMAIN 4..227
FT /note="tr-type G"
FT REGION 13..20
FT /note="G1"
FT /evidence="ECO:0000250"
FT REGION 69..73
FT /note="G2"
FT /evidence="ECO:0000250"
FT REGION 90..93
FT /note="G3"
FT /evidence="ECO:0000250"
FT REGION 152..155
FT /note="G4"
FT /evidence="ECO:0000250"
FT REGION 193..195
FT /note="G5"
FT /evidence="ECO:0000250"
FT BINDING 13..20
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00118"
FT BINDING 90..94
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00118"
FT BINDING 152..155
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00118"
FT VARIANT 196
FT /note="A -> S (in strain: MT-3 and MT-4)"
FT VARIANT 203
FT /note="R -> K (in strain: MT-3 and MT-4)"
FT VARIANT 347
FT /note="I -> L (in strain: MT-3 and MT-4)"
FT CONFLICT 14
FT /note="H -> Q (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 240
FT /note="V -> R (in Ref. 1 and 2)"
FT /evidence="ECO:0000305"
FT STRAND 6..14
FT /evidence="ECO:0007829|PDB:1JNY"
FT HELIX 19..30
FT /evidence="ECO:0007829|PDB:1JNY"
FT HELIX 35..48
FT /evidence="ECO:0007829|PDB:1JNY"
FT HELIX 52..64
FT /evidence="ECO:0007829|PDB:1JNY"
FT STRAND 78..80
FT /evidence="ECO:0007829|PDB:1JNY"
FT STRAND 85..88
FT /evidence="ECO:0007829|PDB:1JNY"
FT STRAND 92..94
FT /evidence="ECO:0007829|PDB:1JNY"
FT HELIX 97..102
FT /evidence="ECO:0007829|PDB:1JNY"
FT STRAND 104..106
FT /evidence="ECO:0007829|PDB:1SKQ"
FT STRAND 109..116
FT /evidence="ECO:0007829|PDB:1JNY"
FT HELIX 121..126
FT /evidence="ECO:0007829|PDB:1JNY"
FT HELIX 131..141
FT /evidence="ECO:0007829|PDB:1JNY"
FT STRAND 148..152
FT /evidence="ECO:0007829|PDB:1JNY"
FT HELIX 154..156
FT /evidence="ECO:0007829|PDB:1JNY"
FT STRAND 157..159
FT /evidence="ECO:0007829|PDB:1JNY"
FT HELIX 163..179
FT /evidence="ECO:0007829|PDB:1JNY"
FT STRAND 188..191
FT /evidence="ECO:0007829|PDB:1JNY"
FT TURN 194..197
FT /evidence="ECO:0007829|PDB:1JNY"
FT HELIX 215..219
FT /evidence="ECO:0007829|PDB:1JNY"
FT HELIX 228..230
FT /evidence="ECO:0007829|PDB:1JNY"
FT STRAND 234..243
FT /evidence="ECO:0007829|PDB:1JNY"
FT TURN 244..246
FT /evidence="ECO:0007829|PDB:1JNY"
FT STRAND 247..253
FT /evidence="ECO:0007829|PDB:1JNY"
FT STRAND 264..268
FT /evidence="ECO:0007829|PDB:1JNY"
FT TURN 269..271
FT /evidence="ECO:0007829|PDB:1JNY"
FT STRAND 272..281
FT /evidence="ECO:0007829|PDB:1JNY"
FT STRAND 284..289
FT /evidence="ECO:0007829|PDB:1JNY"
FT STRAND 294..302
FT /evidence="ECO:0007829|PDB:1JNY"
FT HELIX 304..306
FT /evidence="ECO:0007829|PDB:1JNY"
FT STRAND 312..314
FT /evidence="ECO:0007829|PDB:1JNY"
FT STRAND 322..332
FT /evidence="ECO:0007829|PDB:1JNY"
FT STRAND 346..349
FT /evidence="ECO:0007829|PDB:1JNY"
FT STRAND 352..365
FT /evidence="ECO:0007829|PDB:1JNY"
FT TURN 367..369
FT /evidence="ECO:0007829|PDB:1JNY"
FT STRAND 371..376
FT /evidence="ECO:0007829|PDB:1JNY"
FT STRAND 378..380
FT /evidence="ECO:0007829|PDB:1SKQ"
FT STRAND 385..394
FT /evidence="ECO:0007829|PDB:1JNY"
FT TURN 401..403
FT /evidence="ECO:0007829|PDB:1JNY"
FT HELIX 405..407
FT /evidence="ECO:0007829|PDB:1JNY"
FT STRAND 409..414
FT /evidence="ECO:0007829|PDB:1JNY"
FT STRAND 417..428
FT /evidence="ECO:0007829|PDB:1JNY"
SQ SEQUENCE 435 AA; 48489 MW; 222B540A31768A59 CRC64;
MSQKPHLNLI VIGHIDHGKS TLVGRLLMDR GFIDEKTVKE AEEAAKKLGK ESEKFAFLLD
RLKEERERGV TINLTFMRFE TKKYFFTIID APGHRDFVKN MITGASQADA AILVVSAKKG
EYEAGMSVEG QTREHIILAK TMGLDQLIVA VNKMDLTEPP YDEKRYKEIV DQVSKFMRSY
GFNTNKVRFV PVVAPAGDNI THRSENMKWY NGPTLEEYLD QLELPPKPVD KPLRIPIQDV
YSISGVGTVP VGRVESGVLK VGDKIVFMPA GKVGEVRSIE THHTKMDKAE PGDNIGFNVR
GVEKKDIKRG DVVGHPNNPP TVADEFTARI IVVWHPTALA NGYTPVIHVH TASVACRVSE
LVSKLDPRTG QEAEKNPQFL KQGDVAIVKF KPIKPLCVEK YNEFPPLGRF AMRDMGKTVG
VGIIVDVKPA KVEIK
