EF1A_WHEAT
ID EF1A_WHEAT Reviewed; 447 AA.
AC Q03033;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1993, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Elongation factor 1-alpha;
DE Short=EF-1-alpha;
GN Name=TEF1;
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1398146; DOI=10.1016/0378-1119(92)90113-4;
RA Metz A.M., Timmer R.T., Allen M.L., Browning K.S.;
RT "Sequence of a cDNA encoding the alpha-subunit of wheat translation
RT elongation factor 1.";
RL Gene 120:315-316(1992).
CC -!- FUNCTION: This protein promotes the GTP-dependent binding of aminoacyl-
CC tRNA to the A-site of ribosomes during protein biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A
CC subfamily. {ECO:0000305}.
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DR EMBL; M90077; AAA34306.1; -; mRNA.
DR PIR; JC1454; JC1454.
DR AlphaFoldDB; Q03033; -.
DR SMR; Q03033; -.
DR STRING; 4565.Traes_2AS_BD2A53CC4.1; -.
DR PRIDE; Q03033; -.
DR EnsemblPlants; TraesCAD_scaffold_014265_01G000100.1; TraesCAD_scaffold_014265_01G000100.1; TraesCAD_scaffold_014265_01G000100.
DR EnsemblPlants; TraesCAD_scaffold_014743_01G000200.1; TraesCAD_scaffold_014743_01G000200.1; TraesCAD_scaffold_014743_01G000200.
DR EnsemblPlants; TraesCAD_scaffold_026204_01G000100.1; TraesCAD_scaffold_026204_01G000100.1; TraesCAD_scaffold_026204_01G000100.
DR EnsemblPlants; TraesCAD_scaffold_069685_01G000100.1; TraesCAD_scaffold_069685_01G000100.1; TraesCAD_scaffold_069685_01G000100.
DR EnsemblPlants; TraesCAD_scaffold_076481_01G000100.1; TraesCAD_scaffold_076481_01G000100.1; TraesCAD_scaffold_076481_01G000100.
DR EnsemblPlants; TraesCAD_scaffold_125550_01G000100.1; TraesCAD_scaffold_125550_01G000100.1; TraesCAD_scaffold_125550_01G000100.
DR EnsemblPlants; TraesCAD_scaffold_152968_01G000100.1; TraesCAD_scaffold_152968_01G000100.1; TraesCAD_scaffold_152968_01G000100.
DR EnsemblPlants; TraesCLE_scaffold_006103_01G000300.1; TraesCLE_scaffold_006103_01G000300.1; TraesCLE_scaffold_006103_01G000300.
DR EnsemblPlants; TraesCLE_scaffold_035623_01G000100.1; TraesCLE_scaffold_035623_01G000100.1; TraesCLE_scaffold_035623_01G000100.
DR EnsemblPlants; TraesCLE_scaffold_045092_01G000100.1; TraesCLE_scaffold_045092_01G000100.1; TraesCLE_scaffold_045092_01G000100.
DR EnsemblPlants; TraesCLE_scaffold_055826_01G000100.1; TraesCLE_scaffold_055826_01G000100.1; TraesCLE_scaffold_055826_01G000100.
DR EnsemblPlants; TraesCLE_scaffold_075968_01G000100.1; TraesCLE_scaffold_075968_01G000100.1; TraesCLE_scaffold_075968_01G000100.
DR EnsemblPlants; TraesCLE_scaffold_079577_01G000100.1; TraesCLE_scaffold_079577_01G000100.1; TraesCLE_scaffold_079577_01G000100.
DR EnsemblPlants; TraesCLE_scaffold_093814_01G000100.1; TraesCLE_scaffold_093814_01G000100.1; TraesCLE_scaffold_093814_01G000100.
DR EnsemblPlants; TraesCLE_scaffold_125014_01G000100.1; TraesCLE_scaffold_125014_01G000100.1; TraesCLE_scaffold_125014_01G000100.
DR EnsemblPlants; TraesCS4B02G196800.1; TraesCS4B02G196800.1; TraesCS4B02G196800.
DR EnsemblPlants; TraesCS4B02G196900.1; TraesCS4B02G196900.1; TraesCS4B02G196900.
DR EnsemblPlants; TraesCS4D02G197100.1; TraesCS4D02G197100.1; TraesCS4D02G197100.
DR EnsemblPlants; TraesCS4D02G197200.1; TraesCS4D02G197200.1; TraesCS4D02G197200.
DR EnsemblPlants; TraesCS5B02G418100.1; TraesCS5B02G418100.1; TraesCS5B02G418100.
DR EnsemblPlants; TraesCS5B02G418200.1; TraesCS5B02G418200.1; TraesCS5B02G418200.
DR EnsemblPlants; TraesCS5D02G423300.1; TraesCS5D02G423300.1; TraesCS5D02G423300.
DR EnsemblPlants; TraesCS5D02G423400.1; TraesCS5D02G423400.1; TraesCS5D02G423400.
DR EnsemblPlants; TraesPAR_scaffold_009553_01G000100.1; TraesPAR_scaffold_009553_01G000100.1; TraesPAR_scaffold_009553_01G000100.
DR EnsemblPlants; TraesPAR_scaffold_014712_01G000100.1; TraesPAR_scaffold_014712_01G000100.1; TraesPAR_scaffold_014712_01G000100.
DR EnsemblPlants; TraesPAR_scaffold_031649_01G000100.1; TraesPAR_scaffold_031649_01G000100.1; TraesPAR_scaffold_031649_01G000100.
DR EnsemblPlants; TraesPAR_scaffold_057848_01G000100.1; TraesPAR_scaffold_057848_01G000100.1; TraesPAR_scaffold_057848_01G000100.
DR EnsemblPlants; TraesPAR_scaffold_077571_01G000100.1; TraesPAR_scaffold_077571_01G000100.1; TraesPAR_scaffold_077571_01G000100.
DR EnsemblPlants; TraesPAR_scaffold_128009_01G000100.1; TraesPAR_scaffold_128009_01G000100.1; TraesPAR_scaffold_128009_01G000100.
DR EnsemblPlants; TraesROB_scaffold_012427_01G000200.1; TraesROB_scaffold_012427_01G000200.1; TraesROB_scaffold_012427_01G000200.
DR EnsemblPlants; TraesROB_scaffold_014313_01G000200.1; TraesROB_scaffold_014313_01G000200.1; TraesROB_scaffold_014313_01G000200.
DR EnsemblPlants; TraesROB_scaffold_021614_01G000100.1; TraesROB_scaffold_021614_01G000100.1; TraesROB_scaffold_021614_01G000100.
DR EnsemblPlants; TraesROB_scaffold_025490_01G000100.1; TraesROB_scaffold_025490_01G000100.1; TraesROB_scaffold_025490_01G000100.
DR EnsemblPlants; TraesROB_scaffold_070674_01G000200.1; TraesROB_scaffold_070674_01G000200.1; TraesROB_scaffold_070674_01G000200.
DR EnsemblPlants; TraesROB_scaffold_075100_01G000100.1; TraesROB_scaffold_075100_01G000100.1; TraesROB_scaffold_075100_01G000100.
DR EnsemblPlants; TraesROB_scaffold_084018_01G000100.1; TraesROB_scaffold_084018_01G000100.1; TraesROB_scaffold_084018_01G000100.
DR EnsemblPlants; TraesWEE_scaffold_006663_01G000100.1; TraesWEE_scaffold_006663_01G000100.1; TraesWEE_scaffold_006663_01G000100.
DR EnsemblPlants; TraesWEE_scaffold_009516_01G000200.1; TraesWEE_scaffold_009516_01G000200.1; TraesWEE_scaffold_009516_01G000200.
DR EnsemblPlants; TraesWEE_scaffold_077118_01G000100.1; TraesWEE_scaffold_077118_01G000100.1; TraesWEE_scaffold_077118_01G000100.
DR EnsemblPlants; TraesWEE_scaffold_081998_01G000100.1; TraesWEE_scaffold_081998_01G000100.1; TraesWEE_scaffold_081998_01G000100.
DR EnsemblPlants; TraesWEE_scaffold_094812_01G000100.1; TraesWEE_scaffold_094812_01G000100.1; TraesWEE_scaffold_094812_01G000100.
DR EnsemblPlants; TraesWEE_scaffold_122200_01G000100.1; TraesWEE_scaffold_122200_01G000100.1; TraesWEE_scaffold_122200_01G000100.
DR EnsemblPlants; TraesWEE_scaffold_123700_01G000100.1; TraesWEE_scaffold_123700_01G000100.1; TraesWEE_scaffold_123700_01G000100.
DR Gramene; TraesCAD_scaffold_014265_01G000100.1; TraesCAD_scaffold_014265_01G000100.1; TraesCAD_scaffold_014265_01G000100.
DR Gramene; TraesCAD_scaffold_014743_01G000200.1; TraesCAD_scaffold_014743_01G000200.1; TraesCAD_scaffold_014743_01G000200.
DR Gramene; TraesCAD_scaffold_026204_01G000100.1; TraesCAD_scaffold_026204_01G000100.1; TraesCAD_scaffold_026204_01G000100.
DR Gramene; TraesCAD_scaffold_069685_01G000100.1; TraesCAD_scaffold_069685_01G000100.1; TraesCAD_scaffold_069685_01G000100.
DR Gramene; TraesCAD_scaffold_076481_01G000100.1; TraesCAD_scaffold_076481_01G000100.1; TraesCAD_scaffold_076481_01G000100.
DR Gramene; TraesCAD_scaffold_125550_01G000100.1; TraesCAD_scaffold_125550_01G000100.1; TraesCAD_scaffold_125550_01G000100.
DR Gramene; TraesCAD_scaffold_152968_01G000100.1; TraesCAD_scaffold_152968_01G000100.1; TraesCAD_scaffold_152968_01G000100.
DR Gramene; TraesCLE_scaffold_006103_01G000300.1; TraesCLE_scaffold_006103_01G000300.1; TraesCLE_scaffold_006103_01G000300.
DR Gramene; TraesCLE_scaffold_035623_01G000100.1; TraesCLE_scaffold_035623_01G000100.1; TraesCLE_scaffold_035623_01G000100.
DR Gramene; TraesCLE_scaffold_045092_01G000100.1; TraesCLE_scaffold_045092_01G000100.1; TraesCLE_scaffold_045092_01G000100.
DR Gramene; TraesCLE_scaffold_055826_01G000100.1; TraesCLE_scaffold_055826_01G000100.1; TraesCLE_scaffold_055826_01G000100.
DR Gramene; TraesCLE_scaffold_075968_01G000100.1; TraesCLE_scaffold_075968_01G000100.1; TraesCLE_scaffold_075968_01G000100.
DR Gramene; TraesCLE_scaffold_079577_01G000100.1; TraesCLE_scaffold_079577_01G000100.1; TraesCLE_scaffold_079577_01G000100.
DR Gramene; TraesCLE_scaffold_093814_01G000100.1; TraesCLE_scaffold_093814_01G000100.1; TraesCLE_scaffold_093814_01G000100.
DR Gramene; TraesCLE_scaffold_125014_01G000100.1; TraesCLE_scaffold_125014_01G000100.1; TraesCLE_scaffold_125014_01G000100.
DR Gramene; TraesCS4B02G196800.1; TraesCS4B02G196800.1; TraesCS4B02G196800.
DR Gramene; TraesCS4B02G196900.1; TraesCS4B02G196900.1; TraesCS4B02G196900.
DR Gramene; TraesCS4D02G197100.1; TraesCS4D02G197100.1; TraesCS4D02G197100.
DR Gramene; TraesCS4D02G197200.1; TraesCS4D02G197200.1; TraesCS4D02G197200.
DR Gramene; TraesCS5B02G418100.1; TraesCS5B02G418100.1; TraesCS5B02G418100.
DR Gramene; TraesCS5B02G418200.1; TraesCS5B02G418200.1; TraesCS5B02G418200.
DR Gramene; TraesCS5D02G423300.1; TraesCS5D02G423300.1; TraesCS5D02G423300.
DR Gramene; TraesCS5D02G423400.1; TraesCS5D02G423400.1; TraesCS5D02G423400.
DR Gramene; TraesPAR_scaffold_009553_01G000100.1; TraesPAR_scaffold_009553_01G000100.1; TraesPAR_scaffold_009553_01G000100.
DR Gramene; TraesPAR_scaffold_014712_01G000100.1; TraesPAR_scaffold_014712_01G000100.1; TraesPAR_scaffold_014712_01G000100.
DR Gramene; TraesPAR_scaffold_031649_01G000100.1; TraesPAR_scaffold_031649_01G000100.1; TraesPAR_scaffold_031649_01G000100.
DR Gramene; TraesPAR_scaffold_057848_01G000100.1; TraesPAR_scaffold_057848_01G000100.1; TraesPAR_scaffold_057848_01G000100.
DR Gramene; TraesPAR_scaffold_077571_01G000100.1; TraesPAR_scaffold_077571_01G000100.1; TraesPAR_scaffold_077571_01G000100.
DR Gramene; TraesPAR_scaffold_128009_01G000100.1; TraesPAR_scaffold_128009_01G000100.1; TraesPAR_scaffold_128009_01G000100.
DR Gramene; TraesROB_scaffold_012427_01G000200.1; TraesROB_scaffold_012427_01G000200.1; TraesROB_scaffold_012427_01G000200.
DR Gramene; TraesROB_scaffold_014313_01G000200.1; TraesROB_scaffold_014313_01G000200.1; TraesROB_scaffold_014313_01G000200.
DR Gramene; TraesROB_scaffold_021614_01G000100.1; TraesROB_scaffold_021614_01G000100.1; TraesROB_scaffold_021614_01G000100.
DR Gramene; TraesROB_scaffold_025490_01G000100.1; TraesROB_scaffold_025490_01G000100.1; TraesROB_scaffold_025490_01G000100.
DR Gramene; TraesROB_scaffold_070674_01G000200.1; TraesROB_scaffold_070674_01G000200.1; TraesROB_scaffold_070674_01G000200.
DR Gramene; TraesROB_scaffold_075100_01G000100.1; TraesROB_scaffold_075100_01G000100.1; TraesROB_scaffold_075100_01G000100.
DR Gramene; TraesROB_scaffold_084018_01G000100.1; TraesROB_scaffold_084018_01G000100.1; TraesROB_scaffold_084018_01G000100.
DR Gramene; TraesWEE_scaffold_006663_01G000100.1; TraesWEE_scaffold_006663_01G000100.1; TraesWEE_scaffold_006663_01G000100.
DR Gramene; TraesWEE_scaffold_009516_01G000200.1; TraesWEE_scaffold_009516_01G000200.1; TraesWEE_scaffold_009516_01G000200.
DR Gramene; TraesWEE_scaffold_077118_01G000100.1; TraesWEE_scaffold_077118_01G000100.1; TraesWEE_scaffold_077118_01G000100.
DR Gramene; TraesWEE_scaffold_081998_01G000100.1; TraesWEE_scaffold_081998_01G000100.1; TraesWEE_scaffold_081998_01G000100.
DR Gramene; TraesWEE_scaffold_094812_01G000100.1; TraesWEE_scaffold_094812_01G000100.1; TraesWEE_scaffold_094812_01G000100.
DR Gramene; TraesWEE_scaffold_122200_01G000100.1; TraesWEE_scaffold_122200_01G000100.1; TraesWEE_scaffold_122200_01G000100.
DR Gramene; TraesWEE_scaffold_123700_01G000100.1; TraesWEE_scaffold_123700_01G000100.1; TraesWEE_scaffold_123700_01G000100.
DR eggNOG; KOG0052; Eukaryota.
DR OMA; VACTFES; -.
DR Proteomes; UP000019116; Unplaced.
DR ExpressionAtlas; Q03033; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0003746; F:translation elongation factor activity; IBA:GO_Central.
DR GO; GO:0006412; P:translation; IBA:GO_Central.
DR GO; GO:0006414; P:translational elongation; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00118_A; EF_Tu_A; 1.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR031157; G_TR_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR InterPro; IPR009001; Transl_elong_EF1A/Init_IF2_C.
DR InterPro; IPR004539; Transl_elong_EF1A_euk/arc.
DR InterPro; IPR004160; Transl_elong_EFTu/EF1A_C.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR Pfam; PF03143; GTP_EFTU_D3; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF50447; SSF50447; 1.
DR SUPFAM; SSF50465; SSF50465; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00483; EF-1_alpha; 1.
DR PROSITE; PS00301; G_TR_1; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Elongation factor; GTP-binding; Methylation; Nucleotide-binding;
KW Phosphoprotein; Protein biosynthesis; Reference proteome.
FT CHAIN 1..447
FT /note="Elongation factor 1-alpha"
FT /id="PRO_0000090948"
FT DOMAIN 5..230
FT /note="tr-type G"
FT REGION 14..21
FT /note="G1"
FT /evidence="ECO:0000250"
FT REGION 70..74
FT /note="G2"
FT /evidence="ECO:0000250"
FT REGION 91..94
FT /note="G3"
FT /evidence="ECO:0000250"
FT REGION 153..156
FT /note="G4"
FT /evidence="ECO:0000250"
FT REGION 194..196
FT /note="G5"
FT /evidence="ECO:0000250"
FT BINDING 14..21
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 91..95
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 153..156
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT MOD_RES 55
FT /note="N6,N6-dimethyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q8GTY0"
FT MOD_RES 79
FT /note="N6,N6,N6-trimethyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q8GTY0"
FT MOD_RES 187
FT /note="N6,N6,N6-trimethyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q8GTY0"
FT MOD_RES 261
FT /note="N6-methyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q8GTY0"
FT MOD_RES 289
FT /note="5-glutamyl glycerylphosphorylethanolamine"
FT /evidence="ECO:0000250"
FT MOD_RES 306
FT /note="N6,N6,N6-trimethyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q8GTY0"
FT MOD_RES 362
FT /note="5-glutamyl glycerylphosphorylethanolamine"
FT /evidence="ECO:0000250"
FT MOD_RES 396
FT /note="N6,N6,N6-trimethyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q8GTY0"
SQ SEQUENCE 447 AA; 49169 MW; C09190219A7EE1DB CRC64;
MGKEKTHINI VVIGHVDSGK STTTGHLIYK LGGIDKRVIE RFEKEAAEMN KRSFKYAWVL
DKLKAERERG ITIDIALWKF ETTKYYCTVI DAPGHRDFIK NMITGTSQAD CAVLIIDSTT
GGFEAGISKD GQTREHALLA FTLGVKQMIC CCNKMDATTP KYSKARYEEI VKEVSSYLKK
VGYNPDKVPF VPISGFEGDN MIERSTNLDW YKGPTLLEAL DQINEPKRPS DKPLRLPLQD
VYKIGGIGTV PVGRVETGVI KPGMVVTFGP TGLTTEVKSV EMHHESLLEA LPGDNVGFNV
KNVAVKDLKR GFVASNSKDD PAKEAANFTS QVIIMNHPGQ IGNGYAPVLD CHTSHIAVKF
AELVTKIDRR SGKELEALPK FLKNGDAGIV KMIPTKPMVV ETFATYPPLG RFAVRDMRQT
VAVGVIKGVE KKDPTGAKVT KAAIKKK
