EFC14_MOUSE
ID EFC14_MOUSE Reviewed; 484 AA.
AC Q8BGQ6; A2A8U1; Q3TQN6; Q8BJV7; Q8C0R1;
DT 10-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=EF-hand calcium-binding domain-containing protein 14;
GN Name=Efcab14; Synonyms=Kiaa0494;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J;
RC TISSUE=Brain cortex, Corpora quadrigemina, Eye, Skin, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8BGQ6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BGQ6-2; Sequence=VSP_008512;
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK028618; BAC26034.1; -; mRNA.
DR EMBL; AK030011; BAC26732.1; -; mRNA.
DR EMBL; AK078691; BAC37361.1; -; mRNA.
DR EMBL; AK080626; BAC37965.1; -; mRNA.
DR EMBL; AK163435; BAE37346.1; -; mRNA.
DR EMBL; AL626806; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS89810.1; -. [Q8BGQ6-1]
DR RefSeq; XP_006503069.1; XM_006503006.3. [Q8BGQ6-1]
DR AlphaFoldDB; Q8BGQ6; -.
DR SMR; Q8BGQ6; -.
DR STRING; 10090.ENSMUSP00000074025; -.
DR GlyConnect; 2278; 1 N-Linked glycan (1 site).
DR GlyGen; Q8BGQ6; 1 site, 1 N-linked glycan (1 site).
DR PhosphoSitePlus; Q8BGQ6; -.
DR SwissPalm; Q8BGQ6; -.
DR MaxQB; Q8BGQ6; -.
DR PaxDb; Q8BGQ6; -.
DR PeptideAtlas; Q8BGQ6; -.
DR PRIDE; Q8BGQ6; -.
DR ProteomicsDB; 277443; -. [Q8BGQ6-1]
DR ProteomicsDB; 277444; -. [Q8BGQ6-2]
DR Antibodypedia; 2537; 107 antibodies from 22 providers.
DR Ensembl; ENSMUST00000106522; ENSMUSP00000102132; ENSMUSG00000034210. [Q8BGQ6-2]
DR Ensembl; ENSMUST00000106525; ENSMUSP00000102135; ENSMUSG00000034210. [Q8BGQ6-1]
DR GeneID; 230648; -.
DR UCSC; uc008uff.1; mouse. [Q8BGQ6-1]
DR CTD; 9813; -.
DR MGI; MGI:2442397; Efcab14.
DR VEuPathDB; HostDB:ENSMUSG00000034210; -.
DR eggNOG; ENOG502QSQB; Eukaryota.
DR GeneTree; ENSGT00390000011196; -.
DR InParanoid; Q8BGQ6; -.
DR BioGRID-ORCS; 230648; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Efcab14; mouse.
DR PRO; PR:Q8BGQ6; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q8BGQ6; protein.
DR Bgee; ENSMUSG00000034210; Expressed in animal zygote and 234 other tissues.
DR ExpressionAtlas; Q8BGQ6; baseline and differential.
DR Genevisible; Q8BGQ6; MM.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR042352; EFCAB14.
DR PANTHER; PTHR15717; PTHR15717; 1.
DR SUPFAM; SSF47473; SSF47473; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Calcium; Metal-binding; Reference proteome; Repeat.
FT CHAIN 1..484
FT /note="EF-hand calcium-binding domain-containing protein
FT 14"
FT /id="PRO_0000073883"
FT DOMAIN 423..452
FT /note="EF-hand 1"
FT DOMAIN 453..484
FT /note="EF-hand 2"
FT REGION 1..48
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 227..255
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 313..395
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..21
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 227..249
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 313..363
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 370..395
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 466
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255"
FT BINDING 468
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255"
FT BINDING 470
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255"
FT BINDING 472
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255"
FT BINDING 477
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255"
FT VAR_SEQ 427..484
FT /note="DLQDLFHKTGQDVDGMLTYQELWNSLGSAMPRPESLRAFDSNGDGRYSFLEL
FT RLALGI -> GQKHQILLELELELQAVVNYLTRTLGTELWSAGGGVQTPDH (in
FT isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_008512"
FT CONFLICT 29
FT /note="H -> R (in Ref. 1; BAC37361)"
FT /evidence="ECO:0000305"
FT CONFLICT 174
FT /note="V -> A (in Ref. 1; BAC26732)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 484 AA; 53694 MW; 7D6C188D472CF0FF CRC64;
MKKRKELNAL IGLAGDHRRK KTKQGSGSHR LLRTEPPDSD SESSTDEEEF GAIGNRSRFV
KGDYARCCKI CCPLCAFVIL AACVVASVGL VWMQMALKED LDVLKEKFRT MESNQKSSFQ
EIPKLNEELL SKQKQLEKIE SGELGLSRVW INITEMNKQI SLLSSAVNHL KASVKSAADL
LSLPSTVEGL QKSVASIGNT LNSVHLAVEV IQKTVDEHRT TLGLLQGSME NNGSNQILPS
PSPPSELDNK SHSESAKQDI LYLHNSLEEV NSTVVEYQRQ NDLKLKGMSE TLSNLTQRLS
LIESHVVALS KAEQRTNVSS STMENRAATL KRESLVTNRS DTVQAQSMKK EDNSNSQVSE
LREKLQLISA LTNKPESNRP PETTDEEQVQ NFTSDPSALP EFSQLLRNQI ETQVKPLSLP
GISSIKDLQD LFHKTGQDVD GMLTYQELWN SLGSAMPRPE SLRAFDSNGD GRYSFLELRL
ALGI
