EFCB1_LOTGI
ID EFCB1_LOTGI Reviewed; 224 AA.
AC B3A0Q5;
DT 25-JAN-2012, integrated into UniProtKB/Swiss-Prot.
DT 25-JAN-2012, sequence version 1.
DT 03-AUG-2022, entry version 30.
DE RecName: Full=EF-hand calcium-binding domain-containing protein 1;
DE Flags: Precursor;
OS Lottia gigantea (Giant owl limpet).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC Patellogastropoda; Lottioidea; Lottiidae; Lottia.
OX NCBI_TaxID=225164;
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND IDENTIFICATION.
RA Richardson P., Lucas S., Rokhsar D., Wang M., Lindquist E.A.;
RT "DOE Joint Genome Institute Lottia gigantea EST project.";
RL Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP PROTEIN SEQUENCE OF 106-138 AND 161-191, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RC TISSUE=Shell;
RX PubMed=23145877; DOI=10.1111/febs.12062;
RA Marie B., Jackson D.J., Ramos-Silva P., Zanella-Cleon I., Guichard N.,
RA Marin F.;
RT "The shell-forming proteome of Lottia gigantea reveals both deep
RT conservations and lineage-specific novelties.";
RL FEBS J. 280:214-232(2013).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:23145877}.
CC -!- TISSUE SPECIFICITY: Component of the acid-soluble organic matrix of
CC calcified layers of the shell (at protein level).
CC {ECO:0000269|PubMed:23145877}.
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DR EMBL; FC615346; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_009046539.1; XM_009048291.1.
DR AlphaFoldDB; B3A0Q5; -.
DR EnsemblMetazoa; LotgiT230492; LotgiP230492; LotgiG230492.
DR GeneID; 20248310; -.
DR KEGG; lgi:LOTGIDRAFT_230492; -.
DR CTD; 20248310; -.
DR HOGENOM; CLU_1236276_0_0_1; -.
DR OMA; VACNHAC; -.
DR OrthoDB; 1591636at2759; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13202; EF-hand_5; 2.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 1.
PE 1: Evidence at protein level;
KW Calcium; Direct protein sequencing; Metal-binding; Secreted; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..224
FT /note="EF-hand calcium-binding domain-containing protein 1"
FT /evidence="ECO:0000255"
FT /id="PRO_0000415255"
FT DOMAIN 115..150
FT /note="EF-hand"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 128
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 130
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 132
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 134
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 139
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
SQ SEQUENCE 224 AA; 25132 MW; BFF551E2F8ECA69B CRC64;
MKVSLLLLAL VLVCLVQGSE SWRLRRTLRR IGRGIRRVGR VVKNVACNHA CPRLCRQGVC
KLACNLGCRG KRDVTQQLNQ QGHVTPIPNS FAAYDMNEDG IISRKELALA IGEDIAHPDF
MKAYSIADVD GDGELSPKEF YNGPYVFEMD LNDDDLAYCR YRLDIDDDLI DVIEGELVNQ
APNFIEGNQV KETGKPHEII SKTGQPETKP IDKYVKLAEK TKTQ
