ID   EFCB2_LOTGI             Reviewed;         119 AA.
AC   B3A0R9;
DT   25-JAN-2012, integrated into UniProtKB/Swiss-Prot.
DT   25-JAN-2012, sequence version 1.
DT   03-AUG-2022, entry version 20.
DE   RecName: Full=EF-hand calcium-binding domain-containing protein 2;
DE   Flags: Precursor;
OS   Lottia gigantea (Giant owl limpet).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Patellogastropoda; Lottioidea; Lottiidae; Lottia.
OX   NCBI_TaxID=225164;
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND IDENTIFICATION.
RC   TISSUE=Mantle {ECO:0000269|Ref.1};
RA   Richardson P., Lucas S., Rokhsar D., Wang M., Lindquist E.A.;
RT   "DOE Joint Genome Institute Lottia gigantea EST project.";
RL   Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   PROTEIN SEQUENCE OF 62-77, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC   TISSUE=Shell;
RX   PubMed=23145877; DOI=10.1111/febs.12062;
RA   Marie B., Jackson D.J., Ramos-Silva P., Zanella-Cleon I., Guichard N.,
RA   Marin F.;
RT   "The shell-forming proteome of Lottia gigantea reveals both deep
RT   conservations and lineage-specific novelties.";
RL   FEBS J. 280:214-232(2013).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:23145877}.
CC   -!- TISSUE SPECIFICITY: Component of the acid-soluble organic matrix of
CC       calcified layers of the shell (at protein level).
CC       {ECO:0000269|PubMed:23145877}.
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DR   EMBL; FC621971; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; XP_009051062.1; XM_009052814.1.
DR   AlphaFoldDB; B3A0R9; -.
DR   EnsemblMetazoa; LotgiT231427; LotgiP231427; LotgiG231427.
DR   GeneID; 20248583; -.
DR   KEGG; lgi:LOTGIDRAFT_231427; -.
DR   CTD; 20248583; -.
DR   HOGENOM; CLU_139961_0_0_1; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR   PROSITE; PS00018; EF_HAND_1; 1.
PE   1: Evidence at protein level;
KW   Calcium; Direct protein sequencing; Metal-binding; Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..119
FT                   /note="EF-hand calcium-binding domain-containing protein 2"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000415256"
FT   DOMAIN          82..117
FT                   /note="EF-hand"
FT                   /evidence="ECO:0000255"
FT   BINDING         95
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10142"
FT   BINDING         97
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10142"
FT   BINDING         99
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10142"
FT   BINDING         106
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10142"
SQ   SEQUENCE   119 AA;  13305 MW;  91CBC35AE14328B9 CRC64;
     MKVAVVLIVV LVVMMIGQET DSWRIRIRRG RKIFRKIRPY IPFVIGAVGK RQAGDAEFQA
     KYNAAAEDGV FTDEEIKSVF GVDDNGFVEF KATYDVDGDG VVQVEEYETV VELTENLAG