EFCB5_MOUSE
ID EFCB5_MOUSE Reviewed; 1406 AA.
AC A0JP43; B0QZJ9; Q5NC53; Q8CBN1; Q8CDP1;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 2.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=EF-hand calcium-binding domain-containing protein 5;
GN Name=Efcab5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-150 (ISOFORM 1).
RX PubMed=16183747; DOI=10.1073/pnas.0502132102;
RA Pritsker M., Doniger T.T., Kramer L.C., Westcot S.E., Lemischka I.R.;
RT "Diversification of stem cell molecular repertoire by alternative
RT splicing.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:14290-14295(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 122-1406 (ISOFORM 1), AND
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 374-1406 (ISOFORM 3).
RC STRAIN=C57BL/6J; TISSUE=Testis, and Urinary bladder;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 137-1406 (ISOFORM 2).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=A0JP43-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A0JP43-2; Sequence=VSP_030736, VSP_030737;
CC Name=3;
CC IsoId=A0JP43-3; Sequence=VSP_030738;
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC26618.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAC29157.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AL603842; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL663066; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BX321897; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; DT929470; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK029790; BAC26618.1; ALT_FRAME; mRNA.
DR EMBL; AK035698; BAC29157.1; ALT_FRAME; mRNA.
DR EMBL; BC127154; -; NOT_ANNOTATED_CDS; mRNA.
DR CCDS; CCDS36232.1; -. [A0JP43-1]
DR RefSeq; NP_795939.2; NM_176965.3. [A0JP43-1]
DR AlphaFoldDB; A0JP43; -.
DR STRING; 10090.ENSMUSP00000104037; -.
DR iPTMnet; A0JP43; -.
DR PhosphoSitePlus; A0JP43; -.
DR PaxDb; A0JP43; -.
DR PRIDE; A0JP43; -.
DR ProteomicsDB; 277548; -. [A0JP43-1]
DR ProteomicsDB; 277549; -. [A0JP43-2]
DR ProteomicsDB; 277550; -. [A0JP43-3]
DR Antibodypedia; 15071; 36 antibodies from 14 providers.
DR DNASU; 319634; -.
DR Ensembl; ENSMUST00000108400; ENSMUSP00000104037; ENSMUSG00000050944. [A0JP43-1]
DR GeneID; 319634; -.
DR KEGG; mmu:319634; -.
DR UCSC; uc007kgj.1; mouse. [A0JP43-1]
DR UCSC; uc007kgk.1; mouse. [A0JP43-3]
DR CTD; 374786; -.
DR MGI; MGI:2442440; Efcab5.
DR VEuPathDB; HostDB:ENSMUSG00000050944; -.
DR eggNOG; ENOG502R5M0; Eukaryota.
DR GeneTree; ENSGT00940000154049; -.
DR HOGENOM; CLU_006376_0_0_1; -.
DR InParanoid; A0JP43; -.
DR OMA; PKGMIPM; -.
DR OrthoDB; 1482025at2759; -.
DR PhylomeDB; A0JP43; -.
DR TreeFam; TF329659; -.
DR BioGRID-ORCS; 319634; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Efcab5; mouse.
DR PRO; PR:A0JP43; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; A0JP43; protein.
DR Bgee; ENSMUSG00000050944; Expressed in spermatocyte and 50 other tissues.
DR ExpressionAtlas; A0JP43; baseline and differential.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR Gene3D; 3.30.450.40; -; 1.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR029016; GAF-like_dom_sf.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Calcium; Metal-binding; Reference proteome.
FT CHAIN 1..1406
FT /note="EF-hand calcium-binding domain-containing protein 5"
FT /id="PRO_0000315837"
FT DOMAIN 773..808
FT /note="EF-hand"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT REGION 255..655
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 264..523
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 574..655
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 786
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 788
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 790
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 797
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT VAR_SEQ 1098..1118
FT /note="EDYVLRNMMVTDCLGLAEIHT -> LILGLKSGISGTLSLNSCITG (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_030736"
FT VAR_SEQ 1119..1406
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_030737"
FT VAR_SEQ 1311..1406
FT /note="HINKYLVEEICVLDPTASNVEVNVELVTSYIQAHSRTEVWNFRNIVIELLYH
FT WINICLTLIELNMRQDVSIIPPLPKKSATSIYAISSERSIREKL -> VSLGVISSGQI
FT LTVELAGVRMQESAASKEHPRNPHPSSQVCMRINILKAHLLSFLMTPSDPKPYSPKLSV
FT IPTSDPRDMTVEDLPVWGCVCW (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_030738"
SQ SEQUENCE 1406 AA; 159935 MW; 65D68CDFAB564246 CRC64;
MLMQVEKKKL LSQADIPTKF DPINYLGEYL MRHNTHYIKD PGVSGYQRVM KEITEELKTH
VPDTINNRIS KMKEKVKEKR EQREYISTVK VKVAGMRKQA LEEQFNEWVL NPKGMIPIVV
YISSYITDLK SEVFEEFLKH LCHSAEEFRE IILTDMRRQM FAELFLQCDS GKVMALDRQR
TLALLEAFYD QCSSTTRSLL RNPRQWPFVE FEEIELIEFW GDMDIKKHIY EDFDALLLKM
NMLVAEKLAG KLAENKDLPQ QQRDQELSSD STTEPETATQ LTSQQRSRRV SLTGQGQGKG
PRKTSASKQG ASRGSVAEQG SRRSSGVNQT QQRGSVAEQG SRRSSAVEQT QQRGSVAEQG
SRRSSTVEQT RQRGSVAEQG SRRSSTVEQT QRRGSVAEQG SRRSSGVNQT QQRGSVAEQG
SRRSSAVEQT QQRGSVAEQG SRRSSAMEQE PQTAQDPNSD SLPEQESHRG SITEGSHRGS
ISEPGQRRAS VTGQRRKSSV DDSGSAGSRR GSGTDQGQHR GSVGQRKGSG ERKMSASEYG
PHQESITEEP LTASEPGPQI DTIQELDEDS TPQLEDDSAL KESKTSELTK IETQEEKPLL
LINEEQVPRD SKQPEVPTSS KKERPSGSPK KGRLSGTSKR DSQKDKACEP KPQHVEGKKW
SGEFLICDWK IKHVKSEEEE QAKLICDDTR FTDLHATIRN FQTYKGIKGR SAFNGVSLDL
LQFVQLLETF VGEDTSFSLS KDLASFFQKN YSETKQEKIK ALEQARQNSS RIRRRILLQA
IFEKWDNDGS GFLDLNEVDD LLYTYKEGME RESMKKAKLH INFPEPSPGH EVKLSSKQFQ
RYIELVVSEL RGNEDEVLES VVEFLMGSLE RSHVEGLRNC ARRKWLHQIQ YAAETSGVSL
EPVYTETFRV LTQDAEAHGN KKISAHISLL EENVFLPERG HVLLRNVACT LDDAPFVLNK
VLYRDMKGIS FTVVDEGKPI HVPQVQHHGN IFFWNSFRSK NEYNGSFLAL PLQDAYMRIF
GVLAVDTLRD PHEINIFLPH EIKFYQGVAN AFSTAYHHVH SREHVLHSVM TGIRWLFSVT
SGITTITTCF IEPSSEQEDY VLRNMMVTDC LGLAEIHTDP PTITRNACIF RDFLFKCTDT
SEVILASSGG ETHIAIPLRQ RTKEAMGILD VNIGRSRMLL YQEYKDLQKM VKMIQNVSYE
ILGEFSGEIE KTMVIEMESA GEVKRAGILF FRTMLQELQE CLCLLDSMDF VSLLLYEHKY
HVDSILQDIT LQEVEANVAL VHDVLKGVIL FSQREKDSLS DLEEWEKWKF HINKYLVEEI
CVLDPTASNV EVNVELVTSY IQAHSRTEVW NFRNIVIELL YHWINICLTL IELNMRQDVS
IIPPLPKKSA TSIYAISSER SIREKL
