ID   EFCB_NEPCI              Reviewed;         687 AA.
AC   G9M8X1;
DT   18-APR-2012, integrated into UniProtKB/Swiss-Prot.
DT   22-FEB-2012, sequence version 1.
DT   03-AUG-2022, entry version 25.
DE   RecName: Full=Calcium-binding protein SP84 {ECO:0000303|PubMed:22019571};
DE   AltName: Full=84 kDa salivary protein {ECO:0000303|PubMed:22019571};
DE            Short=NcSP84 {ECO:0000303|PubMed:22019571};
DE   Flags: Precursor;
OS   Nephotettix cincticeps (Green rice leafhopper) (Selenocephalus cincticeps).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Paraneoptera; Hemiptera; Auchenorrhyncha; Membracoidea;
OC   Cicadellidae; Deltocephalinae; Chiasmini; Nephotettix.
OX   NCBI_TaxID=94400;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:BAL41365.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 20-37, FUNCTION,
RP   SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND
RP   CALCIUM-BINDING.
RC   TISSUE=Saliva {ECO:0000269|PubMed:22019571}, and
RC   Salivary gland {ECO:0000269|PubMed:22019571};
RX   PubMed=22019571; DOI=10.1016/j.ibmb.2011.10.001;
RA   Hattori M., Nakamura M., Komatsu S., Tsuchihara K., Tamura Y., Hasegawa T.;
RT   "Molecular cloning of a novel calcium-binding protein in the secreted
RT   saliva of the green rice leafhopper Nephotettix cincticeps.";
RL   Insect Biochem. Mol. Biol. 42:1-9(2012).
CC   -!- FUNCTION: Binds calcium. During feeding of the phloem sap, protein is
CC       injected into sieve tubes of rice plants. This process may suppress the
CC       sieve-element clogging and facilitate continuous ingestion from sieve
CC       tubes. {ECO:0000269|PubMed:22019571, ECO:0000303|PubMed:22019571}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:22019571}.
CC   -!- TISSUE SPECIFICITY: Expressed in salivary glands where expression is
CC       strongest in type III cells in the posterior lobe of the principal
CC       glands (at protein level). Not expressed in midgut, Malpighian tubules
CC       or epidermis. {ECO:0000269|PubMed:22019571}.
CC   -!- DEVELOPMENTAL STAGE: Expressed on days 0-7 after adult emergence at
CC       roughly constant level (at protein level).
CC       {ECO:0000269|PubMed:22019571}.
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DR   EMBL; AB618633; BAL41365.1; -; mRNA.
DR   AlphaFoldDB; G9M8X1; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR   InterPro; IPR002048; EF_hand_dom.
DR   SUPFAM; SSF47473; SSF47473; 2.
DR   PROSITE; PS00018; EF_HAND_1; 1.
DR   PROSITE; PS50222; EF_HAND_2; 5.
PE   1: Evidence at protein level;
KW   Calcium; Direct protein sequencing; Metal-binding; Repeat; Secreted;
KW   Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000269|PubMed:22019571"
FT   CHAIN           20..687
FT                   /note="Calcium-binding protein SP84"
FT                   /evidence="ECO:0000303|PubMed:22019571"
FT                   /id="PRO_0000416970"
FT   DOMAIN          152..187
FT                   /note="EF-hand 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          257..292
FT                   /note="EF-hand 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          406..441
FT                   /note="EF-hand 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          476..511
FT                   /note="EF-hand 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          579..614
FT                   /note="EF-hand 5"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         592
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         594
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         596
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         598
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         603
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
SQ   SEQUENCE   687 AA;  79605 MW;  6E75366A664CA6AC CRC64;
     MMRAIYLLVV VCWAAAANAS SDTVPAEVQT IVKTPGHQYD LYKKMVATIS LPANANTEIH
     KGLNIKRPDN KVTKADIELY YWHKDLKYAV TKFLHLINDD GNKEELTAEE FSNDLQKLAF
     KVALVECHYQ LSPGLRQACY ADEILTYGAL VLESDDITKF YKHLDNDKDN ELKTEEVLKI
     QHTHKNKDNK IVAEELGAYS GAKKDTVTPE EFEEYVTQES IVDDFRECRS KKATEGTTDY
     DNVCLTNELV EYVNDDLTEI DISLLYRSVD TNNDNKIIID ELKAFTGITD DVKAKRILEL
     LDMSATTPAN AKPVVDYGEW RTYLRSPTVL LQLERDCAMK HNDQLEEINC MYKLLKNLLI
     DEHDPIWLEA WDLDHVILFE IFDADKDDGK VFKDDLKKIQ KNFHFKTEAT VTRYMVEADI
     DKDSFICLEE FDEYMDIPHM VYGTGECILH SRTKPDERGD CFVEETSDVV DNFKFIENAA
     FDTWFNHYDT NHNNKIEKEA DGLLAKFNND ANVLTMFLEE TGQGGLTVEP FEFNWYVKQQ
     HLAHAYEDEK IVTALVQKFT AEQITKLHTE LIKYINDEMT ERDIEDLFDE LDHNDDHELT
     QQDFPDCWND VKDLLTHIDA LIPEGDEDTT VNYLEFWAWI NHPNPKKPQE LIKKTFETDC
     TTKHATQKEE VACYVKTFKT KDKFKTA