ID   EFEMO_NEIMB             Reviewed;         388 AA.
AC   Q9K1P6;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Efem/EfeO family lipoprotein NMB0035;
DE   Flags: Precursor;
GN   OrderedLocusNames=NMB0035;
OS   Neisseria meningitidis serogroup B (strain MC58).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC   Neisseria.
OX   NCBI_TaxID=122586;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MC58;
RX   PubMed=10710307; DOI=10.1126/science.287.5459.1809;
RA   Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E.,
RA   Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C.,
RA   Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H.,
RA   Salzberg S.L., White O., Fleischmann R.D., Dougherty B.A., Mason T.M.,
RA   Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D.,
RA   Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V.,
RA   Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M.,
RA   Moxon E.R., Rappuoli R., Venter J.C.;
RT   "Complete genome sequence of Neisseria meningitidis serogroup B strain
RT   MC58.";
RL   Science 287:1809-1815(2000).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=NZ98/254 / Serogroup B;
RX   PubMed=16645985; DOI=10.1002/pmic.200500821;
RA   Vipond C., Suker J., Jones C., Tang C., Feavers I.M., Wheeler J.X.;
RT   "Proteomic analysis of a meningococcal outer membrane vesicle vaccine
RT   prepared from the group B strain NZ98/254.";
RL   Proteomics 6:3400-3413(2006).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-
CC       ProRule:PRU00303}; Lipid-anchor {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC   -!- MISCELLANEOUS: Present in outer membrane vesicle formulations which are
CC       used as vaccines in human.
CC   -!- SIMILARITY: Belongs to the EfeM/EfeO family. {ECO:0000305}.
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DR   EMBL; AE002098; AAF40506.1; -; Genomic_DNA.
DR   PIR; E81244; E81244.
DR   RefSeq; NP_273101.1; NC_003112.2.
DR   RefSeq; WP_002215233.1; NC_003112.2.
DR   AlphaFoldDB; Q9K1P6; -.
DR   SMR; Q9K1P6; -.
DR   STRING; 122586.NMB0035; -.
DR   PaxDb; Q9K1P6; -.
DR   PRIDE; Q9K1P6; -.
DR   EnsemblBacteria; AAF40506; AAF40506; NMB0035.
DR   KEGG; nme:NMB0035; -.
DR   PATRIC; fig|122586.8.peg.48; -.
DR   HOGENOM; CLU_050342_2_1_4; -.
DR   OMA; WTGWHRL; -.
DR   Proteomes; UP000000425; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   CDD; cd14656; Imelysin-like_EfeO; 1.
DR   Gene3D; 1.20.1420.20; -; 1.
DR   Gene3D; 2.60.40.420; -; 1.
DR   InterPro; IPR008972; Cupredoxin.
DR   InterPro; IPR028096; EfeO_Cupredoxin.
DR   InterPro; IPR018976; Imelysin-like.
DR   InterPro; IPR034981; Imelysin-like_EfeO/Algp7.
DR   InterPro; IPR038352; Imelysin_sf.
DR   Pfam; PF13473; Cupredoxin_1; 1.
DR   Pfam; PF09375; Peptidase_M75; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Lipoprotein; Membrane; Palmitate; Reference proteome;
KW   Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           20..388
FT                   /note="Efem/EfeO family lipoprotein NMB0035"
FT                   /id="PRO_0000320326"
FT   LIPID           20
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           20
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   388 AA;  42251 MW;  ECF95AC5A74499C4 CRC64;
     MRKFNLTALS VMLALGLTAC QPPEAEKAAP AASGEAQTAN EGGSVSIAVN DNACEPMELT
     VPSGQVVFNI KNNSGRKLEW EILKGVMVVD ERENIAPGLS DKMTVTLLPG EYEMTCGLLT
     NPRGKLVVTD SGFKDTANEA DLEKLSQPLA DYKAYVQGEV KELVAKTKTF TEAVKAGDIE
     KAKSLFADTR VHYERIEPIA ELFSELDPVI DAREDDFKDG AKDAGFTGFH RIEYALWVEK
     DVSGVKEIAA KLMTDVEALQ KEIDALAFPP GKVVGGASEL IEEVAGSKIS GEEDRYSHTD
     LSDFQANVDG SKKIVDLFRP LIEAKNKALL EKTDTNFKQV NEILAKYRTK DGFETYDKLG
     EADRKALQAS INALAEDLAQ LRGILGLK