ID   EFEM_BACSU              Reviewed;         385 AA.
AC   P39596;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=Probable iron uptake system component EfeM;
DE   Flags: Precursor;
GN   Name=efeM; Synonyms=ywbM; OrderedLocusNames=BSU38270; ORFNames=ipa-28d;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=7934828; DOI=10.1111/j.1365-2958.1993.tb01963.x;
RA   Glaser P., Kunst F., Arnaud M., Coudart M.P., Gonzales W., Hullo M.-F.,
RA   Ionescu M., Lubochinsky B., Marcelino L., Moszer I., Presecan E.,
RA   Santana M., Schneider E., Schweizer J., Vertes A., Rapoport G., Danchin A.;
RT   "Bacillus subtilis genome project: cloning and sequencing of the 97 kb
RT   region from 325 degrees to 333 degrees.";
RL   Mol. Microbiol. 10:371-384(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   FUNCTION IN IRON UPTAKE.
RC   STRAIN=168 / CU1065;
RX   PubMed=16672620; DOI=10.1128/jb.188.10.3664-3673.2006;
RA   Ollinger J., Song K.-B., Antelmann H., Hecker M., Helmann J.D.;
RT   "Role of the Fur regulon in iron transport in Bacillus subtilis.";
RL   J. Bacteriol. 188:3664-3673(2006).
RN   [4]
RP   SUBCELLULAR LOCATION.
RC   STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC   3610 / NRRL NRS-744 / VKM B-501;
RX   PubMed=22882210; DOI=10.1111/j.1365-2958.2012.08205.x;
RA   Yepes A., Schneider J., Mielich B., Koch G., Garcia-Betancur J.C.,
RA   Ramamurthi K.S., Vlamakis H., Lopez D.;
RT   "The biofilm formation defect of a Bacillus subtilis flotillin-defective
RT   mutant involves the protease FtsH.";
RL   Mol. Microbiol. 86:457-471(2012).
CC   -!- FUNCTION: Involved in iron uptake. {ECO:0000269|PubMed:16672620}.
CC   -!- SUBUNIT: Part of a ferrous iron transporter composed of EfeU, EfeM and
CC       EfeN. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane raft {ECO:0000269|PubMed:22882210}.
CC       Note=Present in detergent-resistant membrane (DRM) fractions that may
CC       be equivalent to eukaryotic membrane rafts; these rafts include
CC       proteins involved in signaling, molecule trafficking and protein
CC       secretion. {ECO:0000269|PubMed:22882210}.
CC   -!- SIMILARITY: Belongs to the EfeM/EfeO family. {ECO:0000305}.
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DR   EMBL; X73124; CAA51584.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB15853.1; -; Genomic_DNA.
DR   PIR; S39683; S39683.
DR   RefSeq; NP_391706.1; NC_000964.3.
DR   RefSeq; WP_003243208.1; NZ_JNCM01000034.1.
DR   AlphaFoldDB; P39596; -.
DR   SMR; P39596; -.
DR   STRING; 224308.BSU38270; -.
DR   jPOST; P39596; -.
DR   PaxDb; P39596; -.
DR   PRIDE; P39596; -.
DR   DNASU; 937322; -.
DR   EnsemblBacteria; CAB15853; CAB15853; BSU_38270.
DR   GeneID; 937322; -.
DR   KEGG; bsu:BSU38270; -.
DR   PATRIC; fig|224308.179.peg.4143; -.
DR   eggNOG; COG2822; Bacteria.
DR   InParanoid; P39596; -.
DR   OMA; WTGWHRL; -.
DR   PhylomeDB; P39596; -.
DR   BioCyc; BSUB:BSU38270-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0045121; C:membrane raft; IEA:UniProtKB-SubCell.
DR   CDD; cd14656; Imelysin-like_EfeO; 1.
DR   Gene3D; 1.20.1420.20; -; 1.
DR   InterPro; IPR018976; Imelysin-like.
DR   InterPro; IPR034981; Imelysin-like_EfeO/Algp7.
DR   InterPro; IPR038352; Imelysin_sf.
DR   Pfam; PF09375; Peptidase_M75; 1.
PE   1: Evidence at protein level;
KW   Membrane; Reference proteome; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..385
FT                   /note="Probable iron uptake system component EfeM"
FT                   /id="PRO_0000049954"
SQ   SEQUENCE   385 AA;  42796 MW;  1F9DCB6BA385A3C7 CRC64;
     MNFTKIAVSA GCILALCAGC GANDTSSTKE KASSEKSGVT KEITASVNKM ETIISKLNDS
     VEKGDQKEIE KKGKELNSYW LSFENDIRSD YPFEYTEIEK HLQPIYTEAQ KDKPDAGKIK
     TESESLKASL EDLTEAKKSG KKASDQLAKA ADEYKGYVKE QSDQLVKATE AFTGAVKSGD
     IEKSKTLYAK ARVYYERIEP IAESLGDLDP KIDARENDVE EGDKWTGFHK LEKAIWKDQD
     ISGEKATADQ LLKDVKELDG SIQSLKLTPE QIVAGAMELL NEAGISKITG EEERYSRIDL
     VDLMANVEGS EAVYQTVKSA LVKDHSDLTE KLDTEFSEFE VLMAKYKTND QSYTSYDKLS
     EKQIRELSTK LTTLSETMSK IANVL