EFEO_ECOLI
ID EFEO_ECOLI Reviewed; 375 AA.
AC P0AB24; P75902; Q9R2S4;
DT 11-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Iron uptake system component EfeO;
DE Flags: Precursor;
GN Name=efeO; Synonyms=ycdO; OrderedLocusNames=b1018, JW1003;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=B / BL21;
RX PubMed=10493123;
RX DOI=10.1002/(sici)1522-2683(19990801)20:11<2181::aid-elps2181>3.0.co;2-q;
RA Fountoulakis M., Takacs M.-F., Berndt P., Langen H., Takacs B.;
RT "Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite
RT chromatography.";
RL Electrophoresis 20:2181-2195(1999).
RN [5]
RP INDUCTION.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=12746439; DOI=10.1074/jbc.m303381200;
RA McHugh J.P., Rodriguez-Quinones F., Abdul-Tehrani H., Svistunenko D.A.,
RA Poole R.K., Cooper C.E., Andrews S.C.;
RT "Global iron-dependent gene regulation in Escherichia coli. A new mechanism
RT for iron homeostasis.";
RL J. Biol. Chem. 278:29478-29486(2003).
RN [6]
RP SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=16551627; DOI=10.1074/jbc.m511891200;
RA Sturm A., Schierhorn A., Lindenstrauss U., Lilie H., Brueser T.;
RT "YcdB from Escherichia coli reveals a novel class of Tat-dependently
RT translocated hemoproteins.";
RL J. Biol. Chem. 281:13972-13978(2006).
RN [7]
RP INDUCTION, SUBUNIT, AND LACK OF ACTIVITY IN STRAIN K12.
RC STRAIN=K12;
RX PubMed=17627767; DOI=10.1111/j.1365-2958.2007.05802.x;
RA Cao J., Woodhall M.R., Alvarez J., Cartron M.L., Andrews S.C.;
RT "EfeUOB (YcdNOB) is a tripartite, acid-induced and CpxAR-regulated, low-pH
RT Fe2+ transporter that is cryptic in Escherichia coli K-12 but functional in
RT E. coli O157:H7.";
RL Mol. Microbiol. 65:857-875(2007).
RN [8]
RP FUNCTION, AND DOMAIN.
RC STRAIN=K12;
RX PubMed=19701722; DOI=10.1007/s10534-009-9262-z;
RA Rajasekaran M.B., Nilapwar S., Andrews S.C., Watson K.A.;
RT "EfeO-cupredoxins: major new members of the cupredoxin superfamily with
RT roles in bacterial iron transport.";
RL BioMetals 23:1-17(2010).
CC -!- FUNCTION: Involved in Fe(2+) uptake. Could be an iron-binding and/or
CC electron-transfer component. {ECO:0000269|PubMed:19701722}.
CC -!- SUBUNIT: Monomer. Part of a ferrous iron transporter composed of EfeU,
CC EfeO and EfeB. However, this EfeUOB tripartite iron transporter is
CC defective in E.coli strain K12 due to a frameshift mutation in EfeU.
CC {ECO:0000269|PubMed:16551627, ECO:0000269|PubMed:17627767}.
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000269|PubMed:16551627}.
CC -!- INDUCTION: Repressed by Fur in the presence of iron. Repressed at high
CC pH by the two-component regulatory system CpxA/CpxR.
CC {ECO:0000269|PubMed:12746439, ECO:0000269|PubMed:17627767}.
CC -!- DOMAIN: Contains an N-terminal Cup-like domain and a C-terminal
CC peptidase domain, separated by a flexible linker. Structural modeling
CC identifies 2 potential metal-binding sites in the Cup-like domain, with
CC site I binding Cu(2+) and site II favoring Fe(3+). The peptidase domain
CC may also contain an iron-binding site. {ECO:0000269|PubMed:19701722}.
CC -!- SIMILARITY: Belongs to the EfeM/EfeO family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U00096; AAC74103.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35795.2; -; Genomic_DNA.
DR PIR; H64843; H64843.
DR RefSeq; NP_415537.1; NC_000913.3.
DR RefSeq; WP_000154398.1; NZ_LN832404.1.
DR AlphaFoldDB; P0AB24; -.
DR SMR; P0AB24; -.
DR BioGRID; 4260053; 15.
DR IntAct; P0AB24; 7.
DR STRING; 511145.b1018; -.
DR TCDB; 2.A.108.2.3; the iron/lead transporter (ilt) family.
DR jPOST; P0AB24; -.
DR PaxDb; P0AB24; -.
DR PRIDE; P0AB24; -.
DR EnsemblBacteria; AAC74103; AAC74103; b1018.
DR EnsemblBacteria; BAA35795; BAA35795; BAA35795.
DR GeneID; 58462582; -.
DR GeneID; 945603; -.
DR KEGG; ecj:JW1003; -.
DR KEGG; eco:b1018; -.
DR PATRIC; fig|1411691.4.peg.1251; -.
DR EchoBASE; EB3621; -.
DR eggNOG; COG2822; Bacteria.
DR HOGENOM; CLU_050342_2_1_6; -.
DR InParanoid; P0AB24; -.
DR OMA; WTGWHRL; -.
DR PhylomeDB; P0AB24; -.
DR BioCyc; EcoCyc:G6527-MON; -.
DR BioCyc; MetaCyc:G6527-MON; -.
DR PRO; PR:P0AB24; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0030288; C:outer membrane-bounded periplasmic space; IMP:EcoCyc.
DR GO; GO:0046677; P:response to antibiotic; IMP:EcoCyc.
DR GO; GO:0006979; P:response to oxidative stress; IMP:EcoCyc.
DR GO; GO:0009636; P:response to toxic substance; IMP:EcoCyc.
DR GO; GO:0009411; P:response to UV; IMP:EcoCyc.
DR CDD; cd14656; Imelysin-like_EfeO; 1.
DR Gene3D; 1.20.1420.20; -; 1.
DR Gene3D; 2.60.40.420; -; 1.
DR InterPro; IPR008972; Cupredoxin.
DR InterPro; IPR028096; EfeO_Cupredoxin.
DR InterPro; IPR018976; Imelysin-like.
DR InterPro; IPR034981; Imelysin-like_EfeO/Algp7.
DR InterPro; IPR038352; Imelysin_sf.
DR Pfam; PF13473; Cupredoxin_1; 1.
DR Pfam; PF09375; Peptidase_M75; 1.
DR SUPFAM; SSF49503; SSF49503; 1.
PE 1: Evidence at protein level;
KW Periplasm; Reference proteome; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000305"
FT CHAIN 27..375
FT /note="Iron uptake system component EfeO"
FT /id="PRO_0000168804"
SQ SEQUENCE 375 AA; 41138 MW; A3CA2A106A58F09F CRC64;
MTINFRRNAL QLSVAALFSS AFMANAADVP QVKVTVTDKQ CEPMTITVNA GKTQFIIQNH
SQKALEWEIL KGVMVVEERE NIAPGFSQKM TANLQPGEYD MTCGLLTNPK GKLIVKGEAT
ADAAQSDALL SLGGAITAYK AYVMAETTQL VTDTKAFTDA IKAGDIEKAK ALYAPTRQHY
ERIEPIAELF SDLDGSIDAR EDDYEQKAAD PKFTGFHRLE KALFGDNTTK GMDQYAEQLY
TDVVDLQKRI SELAFPPSKV VGGAAGLIEE VAASKISGEE DRYSHTDLWD FQANVEGSQK
IVDLLRPQLQ KANPELLAKV DANFKKVDTI LAKYRTKDGF ETYDKLTDAD RNALKGPITA
LAEDLAQLRG VLGLD
