ID   EFEU_ECOL6              Reviewed;         276 AA.
AC   Q8FJ36;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 2.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Ferrous iron permease EfeU;
DE   AltName: Full=Fe(2+) ion permease EfeU;
DE   AltName: Full=Ferrous iron uptake protein;
GN   Name=efeU; OrderedLocusNames=c1155;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
RN   [2]
RP   FUNCTION, MUTAGENESIS OF GLU-11 AND GLU-130, INDUCTION, AND TOPOLOGY.
RC   STRAIN=O6:K5:H1 / Nissle 1917;
RX   PubMed=16987175; DOI=10.1111/j.1365-2958.2006.05326.x;
RA   Grosse C., Scherer J., Koch D., Otto M., Taudte N., Grass G.;
RT   "A new ferrous iron-uptake transporter, EfeU (YcdN), from Escherichia
RT   coli.";
RL   Mol. Microbiol. 62:120-131(2006).
CC   -!- FUNCTION: Uptake of Fe(2+) ions across the membrane.
CC       {ECO:0000269|PubMed:16987175}.
CC   -!- SUBUNIT: Part of a ferrous iron transporter composed of EfeU, EfeO and
CC       EfeB. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC   -!- INDUCTION: Expressed in response to iron deprivation at pH 7.
CC       {ECO:0000269|PubMed:16987175}.
CC   -!- SIMILARITY: Belongs to the oxidase-dependent Fe transporter (OFeT) (TC
CC       9.A.10.1) family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAN79623.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AE014075; AAN79623.1; ALT_FRAME; Genomic_DNA.
DR   AlphaFoldDB; Q8FJ36; -.
DR   STRING; 199310.c1155; -.
DR   EnsemblBacteria; AAN79623; AAN79623; c1155.
DR   KEGG; ecc:c1155; -.
DR   eggNOG; COG0672; Bacteria.
DR   HOGENOM; CLU_077905_0_0_6; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0033573; C:high-affinity iron permease complex; IEA:InterPro.
DR   GO; GO:0005381; F:iron ion transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0055072; P:iron ion homeostasis; IEA:UniProtKB-KW.
DR   InterPro; IPR005217; FTR1-like.
DR   InterPro; IPR004923; FTR1/Fip1/EfeU.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   PANTHER; PTHR31632; PTHR31632; 1.
DR   Pfam; PF03239; FTR1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   TIGRFAMs; TIGR00145; TIGR00145; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Ion transport; Iron; Iron transport;
KW   Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..276
FT                   /note="Ferrous iron permease EfeU"
FT                   /id="PRO_0000275154"
FT   TOPO_DOM        1
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        2..22
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        23..34
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        35..55
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        56..69
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        70..90
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        91..118
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        119..139
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        140..147
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        148..168
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        169..179
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        180..200
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        201..244
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        245..265
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        266..276
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   MUTAGEN         11
FT                   /note="E->A: Loss of activity in vivo."
FT                   /evidence="ECO:0000269|PubMed:16987175"
FT   MUTAGEN         130
FT                   /note="E->A: Loss of activity in vivo."
FT                   /evidence="ECO:0000269|PubMed:16987175"
SQ   SEQUENCE   276 AA;  30387 MW;  DE48233A63133045 CRC64;
     MFVPFLIILR EGLEAALIVS LIASYLTRTQ RGRWIGVMWI GVLLAAALCL GLGIFINETT
     GEFPQKEQEL FEGIVAVIAV VILTWMVFWM RKVSRNVKVQ LEQAVDSALQ RGNHHGWALV
     MMVFFAVARE GLESVFFLLA AFQQDVGIWP PLGAMLGLAT AVVLGFLLYW GGIRLNLGAF
     FKWTSLFILF VAAGLAAGAI RAFHEAGLWN HFQEIAFDMS AVLSTHSLFG TLMEGIFGYQ
     EAPSVSEVAV WFIYLIPALV AFVLPPRAGA TASRSM