EFEU_ECOLI
ID EFEU_ECOLI Reviewed; 276 AA.
AC P75901; P75900; Q9R3S6;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-1998, sequence version 2.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Putative inactive ferrous iron permease EfeU;
DE AltName: Full=Putative Fe(2+) ion permease EfeU;
GN Name=efeU; Synonyms=ycdN; OrderedLocusNames=b4490, JW5141/JW1002;
GN ORFNames=b1016/b1017;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP SUBUNIT, AND LACK OF ACTIVITY IN STRAIN K12.
RC STRAIN=K12;
RX PubMed=17627767; DOI=10.1111/j.1365-2958.2007.05802.x;
RA Cao J., Woodhall M.R., Alvarez J., Cartron M.L., Andrews S.C.;
RT "EfeUOB (YcdNOB) is a tripartite, acid-induced and CpxAR-regulated, low-pH
RT Fe2+ transporter that is cryptic in Escherichia coli K-12 but functional in
RT E. coli O157:H7.";
RL Mol. Microbiol. 65:857-875(2007).
CC -!- SUBUNIT: Part of a ferrous iron transporter composed of EfeU, EfeO and
CC EfeB. However, this EfeUOB tripartite iron transporter is defective in
CC E.coli strain K12 due to a frameshift mutation in EfeU.
CC {ECO:0000269|PubMed:17627767}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the oxidase-dependent Fe transporter (OFeT) (TC
CC 9.A.10.1) family. {ECO:0000305}.
CC -!- CAUTION: Could be the product of a pseudogene. Strain K12 contains a
CC truncated, inactive permease due to a frameshift in position 34 that
CC produces two separate ORFs. The sequence shown here is a reconstruction
CC originating from EcoGene. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AP009048; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=U00096; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; U00096; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AP009048; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR TCDB; 2.A.108.2.3; the iron/lead transporter (ilt) family.
DR PRIDE; P75901; -.
DR EchoBASE; EB3620; -.
DR InParanoid; P75901; -.
DR PhylomeDB; P75901; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0033573; C:high-affinity iron permease complex; IEA:InterPro.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015093; F:ferrous iron transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0034755; P:iron ion transmembrane transport; IBA:GO_Central.
DR InterPro; IPR005217; FTR1-like.
DR InterPro; IPR004923; FTR1/Fip1/EfeU.
DR PANTHER; PTHR31632; PTHR31632; 1.
DR Pfam; PF03239; FTR1; 1.
DR TIGRFAMs; TIGR00145; TIGR00145; 1.
PE 5: Uncertain;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..276
FT /note="Putative inactive ferrous iron permease EfeU"
FT /id="PRO_0000159652"
FT TOPO_DOM 1
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 2..22
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 23..35
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 57..69
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 70..90
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 91..118
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 119..139
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 140..147
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 148..168
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 169..179
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 180..200
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 201..244
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 245..265
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 266..276
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 276 AA; 30292 MW; 27FC648B7F791AE7 CRC64;
MFVPFLIMLR EGLEAALIVS LIASYLKRTQ RGMXDCVMWI GVLLAAALCL GLGIFINETT
GEFPQKEQEL FEGIVAVIAV VILTWMVFWM RKVSRNVKVQ LEQAVDSALQ RGNHHGWALV
MMVFFAVARE GLESVFFLLA AFQQDVGIWP PLGAMLGLAT AVVLGFLLYW GGIRLNLGAF
FKWTSLFILF VAAGLAAGAI RAFHEAGLWN HFQEIAFDMS AVLSTHSLFG TLMEGIFGYQ
EAPSVSEVAV WFIYLIPALV AFALPPRAGA TASRSA
