EFGM_ORYSJ
ID EFGM_ORYSJ Reviewed; 757 AA.
AC Q9FE64; Q10I39; Q94I51; Q94LE1;
DT 27-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT 27-JUN-2006, sequence version 2.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Elongation factor G, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03061};
DE Short=EF-Gmt {ECO:0000255|HAMAP-Rule:MF_03061};
DE AltName: Full=Elongation factor G 1, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03061};
DE Short=mEF-G 1 {ECO:0000255|HAMAP-Rule:MF_03061};
DE AltName: Full=Elongation factor G1 {ECO:0000255|HAMAP-Rule:MF_03061};
GN OrderedLocusNames=Os03g0565500, LOC_Os03g36780; ORFNames=OSJNBa0026A15.1;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=11328648; DOI=10.3109/10425170009033990;
RA Kato A., Fujita S., Komeda Y.;
RT "Identification and characterization of the gene encoding the mitochondrial
RT elongation factor G in rice.";
RL DNA Seq. 11:395-404(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
CC -!- FUNCTION: Mitochondrial GTPase that catalyzes the GTP-dependent
CC ribosomal translocation step during translation elongation. During this
CC step, the ribosome changes from the pre-translocational (PRE) to the
CC post-translocational (POST) state as the newly formed A-site-bound
CC peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E
CC sites, respectively. Catalyzes the coordinated movement of the two tRNA
CC molecules, the mRNA and conformational changes in the ribosome.
CC {ECO:0000255|HAMAP-Rule:MF_03061}.
CC -!- PATHWAY: Protein biosynthesis; polypeptide chain elongation.
CC {ECO:0000255|HAMAP-Rule:MF_03061}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion.
CC -!- MISCELLANEOUS: This protein may be expected to contain an N-terminal
CC transit peptide but none has been predicted. {ECO:0000255|HAMAP-
CC Rule:MF_03061}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. EF-G/EF-2
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK53868.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB040051; BAB13514.1; -; mRNA.
DR EMBL; AB040052; BAB13515.1; -; Genomic_DNA.
DR EMBL; AC016781; AAK53868.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC084404; AAK50578.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF97151.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF12415.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS84953.1; -; Genomic_DNA.
DR RefSeq; XP_015631992.1; XM_015776506.1.
DR AlphaFoldDB; Q9FE64; -.
DR SMR; Q9FE64; -.
DR STRING; 4530.OS03T0565500-01; -.
DR PaxDb; Q9FE64; -.
DR PRIDE; Q9FE64; -.
DR EnsemblPlants; Os03t0565500-01; Os03t0565500-01; Os03g0565500.
DR GeneID; 4333268; -.
DR Gramene; Os03t0565500-01; Os03t0565500-01; Os03g0565500.
DR KEGG; osa:4333268; -.
DR eggNOG; KOG0465; Eukaryota.
DR HOGENOM; CLU_002794_4_0_1; -.
DR InParanoid; Q9FE64; -.
DR OMA; FRVHRDE; -.
DR OrthoDB; 637899at2759; -.
DR UniPathway; UPA00345; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR Genevisible; Q9FE64; OS.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0003746; F:translation elongation factor activity; IBA:GO_Central.
DR GO; GO:0070125; P:mitochondrial translational elongation; IBA:GO_Central.
DR CDD; cd16262; EFG_III; 1.
DR CDD; cd01434; EFG_mtEFG1_IV; 1.
DR CDD; cd04097; mtEFG1_C; 1.
DR Gene3D; 3.30.230.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00054_B; EF_G_EF_2_B; 1.
DR InterPro; IPR045044; EFG1-like.
DR InterPro; IPR041095; EFG_II.
DR InterPro; IPR009022; EFG_III.
DR InterPro; IPR035647; EFG_III/V.
DR InterPro; IPR035649; EFG_V.
DR InterPro; IPR000640; EFG_V-like.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR031157; G_TR_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR InterPro; IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR InterPro; IPR004540; Transl_elong_EFG/EF2.
DR InterPro; IPR005517; Transl_elong_EFG/EF2_IV.
DR PANTHER; PTHR43636; PTHR43636; 1.
DR Pfam; PF00679; EFG_C; 1.
DR Pfam; PF14492; EFG_III; 1.
DR Pfam; PF03764; EFG_IV; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SMART; SM00838; EFG_C; 1.
DR SMART; SM00889; EFG_IV; 1.
DR SUPFAM; SSF50447; SSF50447; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF54211; SSF54211; 1.
DR SUPFAM; SSF54980; SSF54980; 2.
DR TIGRFAMs; TIGR00484; EF-G; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS00301; G_TR_1; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 2: Evidence at transcript level;
KW Elongation factor; GTP-binding; Mitochondrion; Nucleotide-binding;
KW Protein biosynthesis; Reference proteome.
FT CHAIN 1..757
FT /note="Elongation factor G, mitochondrial"
FT /id="PRO_0000007447"
FT DOMAIN 66..344
FT /note="tr-type G"
FT BINDING 75..82
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03061"
FT BINDING 142..146
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03061"
FT BINDING 196..199
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03061"
FT CONFLICT 45
FT /note="S -> F (in Ref. 1; BAB13514/BAB13515)"
FT /evidence="ECO:0000305"
FT CONFLICT 156..158
FT /note="ALR -> RLG (in Ref. 1; BAB13514/BAB13515)"
FT /evidence="ECO:0000305"
FT CONFLICT 257..259
FT /note="ASD -> RIC (in Ref. 1; BAB13514/BAB13515)"
FT /evidence="ECO:0000305"
FT CONFLICT 347
FT /note="S -> T (in Ref. 1; BAB13514/BAB13515)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 757 AA; 83925 MW; C3E7C7068ACD7B93 CRC64;
MAMARRSASR LLSSFRPFSL LLQPLDDAPS LSAAAAAASA RRGMSSASAL RARDEKEVAR
WRESMDRMRN IGISAHIDSG KTTLTERVLY YTGRIHEIHE VRGRDGVGAK MDSMDLEREK
GITIQSAATY CTWNGYQVNI IDTPGHVDFT IEVERALRVL DGAILVLCSV GGVQSQSITV
DRQMRRYEIP RVAFINKLDR MGADPWKVLN QARSKLRHHN AAVQVPIGLE EEFEGLVDLV
ELKAYKFEGG SGQNVVASDV PSNMQDLVME KRRELIEVVS EVDDQLAEAF LNDEPIQANQ
LKAAIRRATV ARKFIPVYMG SAFKNKGVQP LLDGVLDYLP CPMEVESYAL DQNKSEEKVL
LAGTPAEPLV ALAFKLEEGR FGQLTYLRIY DGVIRKGDFI YNVNTGKKIK VPRLVRMHSN
EMEDIQEAHA GQIVAVFGVD CASGDTFTDG SVKYTMTSMN VPEPVMSLAV SPISKDSGGQ
FSKALNRFQK EDPTFRVGLD PESGETIISG MGELHLDIYV ERIRREYKVD AKVGKPRVNF
RETITQRAEF DYLHKKQSGG QGQYGRVCGY IEPLPSESDG KFEFDNMIIG QAIPSNFIPA
IEKGFKEACN SGSLIGHPVE NIRIVLTDGA SHAVDSSELA FKLASIYAFR QCYAAARPVI
LEPVMKVELK VPTEFQGTVT GDMNKRKGII VGNDQEGDDT VVVCHVPLNN MFGYSTALRS
MTQGKGEFSM EYLEHNTVSQ DVQMQLVNTY KASRGTE
