ADTRP_MESAU
ID ADTRP_MESAU Reviewed; 231 AA.
AC Q60534;
DT 10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 60.
DE RecName: Full=Androgen-dependent TFPI-regulating protein;
DE AltName: Full=Androgen-dependent-expressed protein FAR-17a;
DE AltName: Full=Fatty acid esters of hydroxy fatty acids hydrolase ADTRP {ECO:0000250|UniProtKB:Q96IZ2};
DE Short=FAHFA hydrolase ADTRP {ECO:0000250|UniProtKB:Q96IZ2};
DE EC=3.1.-.- {ECO:0000250|UniProtKB:Q96IZ2};
GN Name=ADTRP;
OS Mesocricetus auratus (Golden hamster).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea;
OC Cricetidae; Cricetinae; Mesocricetus.
OX NCBI_TaxID=10036;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, AND TISSUE SPECIFICITY.
RC TISSUE=Flank organ;
RX PubMed=2045681; DOI=10.1111/1523-1747.ep12475453;
RA Seki T., Ideta R., Shibuya M., Adachi K.;
RT "Isolation and characterization of cDNA for an androgen-regulated mRNA in
RT the flank organ of hamsters.";
RL J. Invest. Dermatol. 96:926-931(1991).
CC -!- FUNCTION: Hydrolyzes bioactive fatty-acid esters of hydroxy-fatty acids
CC (FAHFAs), but not other major classes of lipids (By similarity). Shows
CC a preference for FAHFAs with branching distal from the carboxylate head
CC group of the lipids (By similarity). Regulates the expression and the
CC cell-associated anticoagulant activity of the inhibitor TFPI in
CC endothelial cells (in vitro) (By similarity).
CC {ECO:0000250|UniProtKB:Q96IZ2}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9-hexadecanoyloxy-octadecanoate + H2O = 9-hydroxy-
CC octadecanoate + H(+) + hexadecanoate; Xref=Rhea:RHEA:52052,
CC ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:83670, ChEBI:CHEBI:136286;
CC Evidence={ECO:0000250|UniProtKB:Q96IZ2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52053;
CC Evidence={ECO:0000250|UniProtKB:Q96IZ2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=12-hexadecanoyloxy-octadecanoate + H2O = 12-
CC hydroxyoctadecanoate + H(+) + hexadecanoate; Xref=Rhea:RHEA:52056,
CC ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:83677, ChEBI:CHEBI:84201;
CC Evidence={ECO:0000250|UniProtKB:Q96IZ2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52057;
CC Evidence={ECO:0000250|UniProtKB:Q96IZ2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9-(9Z-hexadecenoyloxy)-octadecanoate + H2O = (9Z)-
CC hexadecenoate + 9-hydroxy-octadecanoate + H(+); Xref=Rhea:RHEA:52068,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32372,
CC ChEBI:CHEBI:136286, ChEBI:CHEBI:136309;
CC Evidence={ECO:0000250|UniProtKB:Q96IZ2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52069;
CC Evidence={ECO:0000250|UniProtKB:Q96IZ2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=12-(9Z-hexadecenoyloxy)-octadecanoate + H2O = (9Z)-
CC hexadecenoate + 12-hydroxyoctadecanoate + H(+); Xref=Rhea:RHEA:52072,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32372,
CC ChEBI:CHEBI:84201, ChEBI:CHEBI:136312;
CC Evidence={ECO:0000250|UniProtKB:Q96IZ2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52073;
CC Evidence={ECO:0000250|UniProtKB:Q96IZ2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=13-(9Z-hexadecenoyloxy)-octadecanoate + H2O = (9Z)-
CC hexadecenoate + 13-hydroxy-octadecanoate + H(+);
CC Xref=Rhea:RHEA:52076, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:32372, ChEBI:CHEBI:136304, ChEBI:CHEBI:136315;
CC Evidence={ECO:0000250|UniProtKB:Q96IZ2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52077;
CC Evidence={ECO:0000250|UniProtKB:Q96IZ2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9-octadecanoyloxy-octadecanoate + H2O = 9-hydroxy-
CC octadecanoate + H(+) + octadecanoate; Xref=Rhea:RHEA:52096,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:25629,
CC ChEBI:CHEBI:136286, ChEBI:CHEBI:136373;
CC Evidence={ECO:0000250|UniProtKB:Q96IZ2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52097;
CC Evidence={ECO:0000250|UniProtKB:Q96IZ2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=12-octadecanoyloxy-octadecanoate + H2O = 12-
CC hydroxyoctadecanoate + H(+) + octadecanoate; Xref=Rhea:RHEA:52080,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:25629,
CC ChEBI:CHEBI:84201, ChEBI:CHEBI:136330;
CC Evidence={ECO:0000250|UniProtKB:Q96IZ2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52081;
CC Evidence={ECO:0000250|UniProtKB:Q96IZ2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=13-octadecanoyloxy-octadecanoate + H2O = 13-hydroxy-
CC octadecanoate + H(+) + octadecanoate; Xref=Rhea:RHEA:52084,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:25629,
CC ChEBI:CHEBI:136304, ChEBI:CHEBI:136335;
CC Evidence={ECO:0000250|UniProtKB:Q96IZ2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52085;
CC Evidence={ECO:0000250|UniProtKB:Q96IZ2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=9-(9Z-octadecenoyloxy)-octadecanoate + H2O = (9Z)-
CC octadecenoate + 9-hydroxy-octadecanoate + H(+); Xref=Rhea:RHEA:52048,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823,
CC ChEBI:CHEBI:136282, ChEBI:CHEBI:136286;
CC Evidence={ECO:0000250|UniProtKB:Q96IZ2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52049;
CC Evidence={ECO:0000250|UniProtKB:Q96IZ2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=12-(9Z-octadecenoyloxy)-octadecanoate + H2O = (9Z)-
CC octadecenoate + 12-hydroxyoctadecanoate + H(+); Xref=Rhea:RHEA:52060,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823,
CC ChEBI:CHEBI:84201, ChEBI:CHEBI:136302;
CC Evidence={ECO:0000250|UniProtKB:Q96IZ2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52061;
CC Evidence={ECO:0000250|UniProtKB:Q96IZ2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=13-(9Z-octadecenoyloxy)-octadecanoate + H2O = (9Z)-
CC octadecenoate + 13-hydroxy-octadecanoate + H(+);
CC Xref=Rhea:RHEA:52064, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30823, ChEBI:CHEBI:136303, ChEBI:CHEBI:136304;
CC Evidence={ECO:0000250|UniProtKB:Q96IZ2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52065;
CC Evidence={ECO:0000250|UniProtKB:Q96IZ2};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5-(9Z-octadecenoyloxy)-octadecanoate + H2O = (9Z)-
CC octadecenoate + 5-hydroxy-octadecanoate + H(+); Xref=Rhea:RHEA:52100,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823,
CC ChEBI:CHEBI:136370, ChEBI:CHEBI:136389;
CC Evidence={ECO:0000250|UniProtKB:Q96IZ2};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52101;
CC Evidence={ECO:0000250|UniProtKB:Q96IZ2};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q96IZ2};
CC Multi-pass membrane protein {ECO:0000255}. Note=Colocalized with TFPI
CC and CAV1 in lipid rafts. {ECO:0000250|UniProtKB:Q96IZ2}.
CC -!- TISSUE SPECIFICITY: Highly expressed in flank organs and weakly in
CC testis and earlobes. {ECO:0000269|PubMed:2045681}.
CC -!- INDUCTION: Up-regulated by androgen. {ECO:0000269|PubMed:2045681}.
CC -!- MISCELLANEOUS: Expression was reduced after castration and reappeared
CC after testosterone treatments.
CC -!- SIMILARITY: Belongs to the AIG1 family. {ECO:0000305}.
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DR EMBL; M80427; AAA37058.1; -; mRNA.
DR PIR; A54313; A54313.
DR RefSeq; NP_001297502.1; NM_001310573.1.
DR RefSeq; XP_012966032.1; XM_013110578.1.
DR AlphaFoldDB; Q60534; -.
DR GeneID; 106020812; -.
DR CTD; 84830; -.
DR OrthoDB; 1482757at2759; -.
DR Proteomes; UP000189706; Unplaced.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR InterPro; IPR006838; Far-17a_AIG1.
DR PANTHER; PTHR10989; PTHR10989; 1.
DR Pfam; PF04750; Far-17a_AIG1; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Hydrolase; Lipid metabolism; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..231
FT /note="Androgen-dependent TFPI-regulating protein"
FT /id="PRO_0000190101"
FT TOPO_DOM 1..7
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q96IZ2"
FT TRANSMEM 8..28
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 29..45
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:Q96IZ2"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 67..85
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q96IZ2"
FT TRANSMEM 86..106
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 107..123
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:Q96IZ2"
FT TRANSMEM 124..144
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 145..154
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q96IZ2"
FT TRANSMEM 155..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 173..190
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:Q96IZ2"
FT TRANSMEM 191..211
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 212..231
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q96IZ2"
FT SITE 47
FT /note="Important for catalytic activity"
FT /evidence="ECO:0000250|UniProtKB:Q96IZ2"
FT SITE 131
FT /note="Important for catalytic activity"
FT /evidence="ECO:0000250|UniProtKB:Q96IZ2"
SQ SEQUENCE 231 AA; 27216 MW; 3275BAD040889A31 CRC64;
MTRTTTCVYH FLVWNWYIFL NYYIPLIGKD DEKLKEFHDG GRSKYLTLLN LLLQAIFFGV
ACLDDVLKRI IGRKDIKFIT STRDLLFSTL VFPISTFIFL VFWTLFYYDR SLIYPKGLDD
YFPAWLNHAM HTYILLFVLV ETILRPHHYP SKKLGLALLG ACNLAYITRV LWRYSQTGNW
VYPVFASLNP LGIIIFFLVC YILNASIYLV GEKINHWKWG ATVKPLMKKK K
