ADY2_YEAST
ID ADY2_YEAST Reviewed; 283 AA.
AC P25613; D6VR19;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1992, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Accumulation of dyads protein 2;
DE AltName: Full=Ammonia transport outward protein 1;
GN Name=ADY2; Synonyms=ATO1; OrderedLocusNames=YCR010C; ORFNames=YCR10C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1626432; DOI=10.1002/yea.320080508;
RA Skala J., Purnelle B., Goffeau A.;
RT "The complete sequence of a 10.8 kb segment distal of SUF2 on the right arm
RT of chromosome III from Saccharomyces cerevisiae reveals seven open reading
RT frames including the RVS161, ADP1 and PGK genes.";
RL Yeast 8:409-417(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=1574125; DOI=10.1038/357038a0;
RA Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M.,
RA Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G.,
RA Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A.,
RA Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M., Carcano C.,
RA Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M.,
RA Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C.,
RA Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F.,
RA Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C.,
RA Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E.,
RA Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P.,
RA Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J.,
RA Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P.,
RA Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M.,
RA Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P.,
RA Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G.,
RA Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E.,
RA Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F.,
RA Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L.,
RA Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J.,
RA Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M.,
RA Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A.,
RA Richterich P., Roberts A.B., Rodriguez F., Sanz E.,
RA Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J.,
RA Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I.,
RA Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M.,
RA Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M.,
RA Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D.,
RA Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K.,
RA Sgouros J.G.;
RT "The complete DNA sequence of yeast chromosome III.";
RL Nature 357:38-46(1992).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [5]
RP FUNCTION.
RX PubMed=11470404; DOI=10.1016/s0960-9822(01)00274-3;
RA Rabitsch K.P., Toth A., Galova M., Schleiffer A., Schaffner G., Aigner E.,
RA Rupp C., Penkner A.M., Moreno-Borchart A.C., Primig M., Esposito R.E.,
RA Klein F., Knop M., Nasmyth K.;
RT "A screen for genes required for meiosis and spore formation based on
RT whole-genome expression.";
RL Curr. Biol. 11:1001-1009(2001).
RN [6]
RP FUNCTION.
RX PubMed=12429834; DOI=10.1091/mbc.e01-12-0149;
RA Palkova Z., Devaux F., Icicova M., Minarikova L., Le Crom S., Jacq C.;
RT "Ammonia pulses and metabolic oscillations guide yeast colony
RT development.";
RL Mol. Biol. Cell 13:3901-3914(2002).
RN [7]
RP FUNCTION.
RX PubMed=14968426; DOI=10.1002/yea.1056;
RA Paiva S., Devaux F., Barbosa S., Jacq C., Casal M.;
RT "Ady2p is essential for the acetate permease activity in the yeast
RT Saccharomyces cerevisiae.";
RL Yeast 21:201-210(2004).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=16286509; DOI=10.1083/jcb.200507168;
RA Taxis C., Keller P., Kavagiou Z., Jensen L.J., Colombelli J., Bork P.,
RA Stelzer E.H.K., Knop M.;
RT "Spore number control and breeding in Saccharomyces cerevisiae: a key role
RT for a self-organizing system.";
RL J. Cell Biol. 171:627-640(2005).
RN [9]
RP SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=17395151; DOI=10.1016/j.bbamem.2007.02.011;
RA Ricicova M., Kucerova H., Vachova L., Palkova Z.;
RT "Association of putative ammonium exporters Ato with detergent-resistant
RT compartments of plasma membrane during yeast colony development: pH affects
RT Ato1p localisation in patches.";
RL Biochim. Biophys. Acta 1768:1170-1178(2007).
RN [10]
RP FUNCTION.
RX PubMed=17233767; DOI=10.1111/j.1567-1364.2006.00191.x;
RA Gentsch M., Kuschel M., Schlegel S., Barth G.;
RT "Mutations at different sites in members of the Gpr1/Fun34/YaaH protein
RT family cause hypersensitivity to acetic acid in Saccharomyces cerevisiae as
RT well as in Yarrowia lipolytica.";
RL FEMS Yeast Res. 7:380-390(2007).
RN [11]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 76625 / YPH499;
RX PubMed=17761666; DOI=10.1074/mcp.m700098-mcp200;
RA Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B.,
RA van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C.;
RT "Profiling phosphoproteins of yeast mitochondria reveals a role of
RT phosphorylation in assembly of the ATP synthase.";
RL Mol. Cell. Proteomics 6:1896-1906(2007).
CC -!- FUNCTION: Transporter protein required for ammonia export and acetate
CC uptake and resistance. Necessary for up-regulation and down-regulation
CC of meiotic plaque (MP) component levels in a dependency on external
CC acetate. Has a role in ascus formation. {ECO:0000269|PubMed:11470404,
CC ECO:0000269|PubMed:12429834, ECO:0000269|PubMed:14968426,
CC ECO:0000269|PubMed:16286509, ECO:0000269|PubMed:17233767}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:16286509,
CC ECO:0000269|PubMed:17395151}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:16286509, ECO:0000269|PubMed:17395151}. Vacuole
CC membrane {ECO:0000269|PubMed:16286509}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:16286509}. Note=Localizes to large detergent
CC resistant patches of the cell membrane (DRM) enriched in ergosterol and
CC sphingolipids (PubMed:17395151).
CC -!- INDUCTION: During meiosis and by external ammonia.
CC {ECO:0000269|PubMed:16286509, ECO:0000269|PubMed:17395151}.
CC -!- SIMILARITY: Belongs to the acetate uptake transporter (AceTr) (TC
CC 2.A.96) family. {ECO:0000305}.
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DR EMBL; X59720; CAA42327.1; -; Genomic_DNA.
DR EMBL; BK006937; DAA07488.1; -; Genomic_DNA.
DR PIR; S19420; S19420.
DR RefSeq; NP_009936.1; NM_001178723.1.
DR AlphaFoldDB; P25613; -.
DR SMR; P25613; -.
DR BioGRID; 30989; 72.
DR DIP; DIP-7894N; -.
DR IntAct; P25613; 4.
DR MINT; P25613; -.
DR STRING; 4932.YCR010C; -.
DR TCDB; 2.A.96.1.4; the acetate uptake transporter (acetr) family.
DR CarbonylDB; P25613; -.
DR iPTMnet; P25613; -.
DR PaxDb; P25613; -.
DR PRIDE; P25613; -.
DR EnsemblFungi; YCR010C_mRNA; YCR010C; YCR010C.
DR GeneID; 850368; -.
DR KEGG; sce:YCR010C; -.
DR SGD; S000000603; ADY2.
DR VEuPathDB; FungiDB:YCR010C; -.
DR eggNOG; ENOG502QUJS; Eukaryota.
DR GeneTree; ENSGT00940000176398; -.
DR HOGENOM; CLU_051062_0_0_1; -.
DR InParanoid; P25613; -.
DR OMA; WKKGNTF; -.
DR BioCyc; YEAST:G3O-29327-MON; -.
DR PRO; PR:P25613; -.
DR Proteomes; UP000002311; Chromosome III.
DR RNAct; P25613; protein.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015123; F:acetate transmembrane transporter activity; IMP:SGD.
DR GO; GO:0008519; F:ammonium transmembrane transporter activity; IMP:SGD.
DR GO; GO:0006846; P:acetate transport; IMP:SGD.
DR GO; GO:0072488; P:ammonium transmembrane transport; IMP:SGD.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0019740; P:nitrogen utilization; IMP:SGD.
DR GO; GO:0055085; P:transmembrane transport; IMP:SGD.
DR InterPro; IPR000791; Gpr1/Fun34/SatP.
DR Pfam; PF01184; Gpr1_Fun34_YaaH; 1.
DR PROSITE; PS01114; GPR1_FUN34_YAAH; 1.
PE 1: Evidence at protein level;
KW Ammonia transport; Cell membrane; Ion transport; Meiosis; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..283
FT /note="Accumulation of dyads protein 2"
FT /id="PRO_0000135706"
FT TOPO_DOM 1..89
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 90..110
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 111..120
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..141
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 142..151
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 173..185
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 186..206
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 207..208
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 209..229
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 230..240
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 241..261
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 262..283
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..41
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..17
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 283 AA; 30726 MW; 36DE421B09D8BB40 CRC64;
MSDKEQTSGN TDLENAPAGY YSSHDNDVNG VAEDERPSHD SLGKIYTGGD NNEYIYIGRQ
KFLKSDLYQA FGGTLNPGLA PAPVHKFANP APLGLSAFAL TTFVLSMFNA RAQGITVPNV
VVGCAMFYGG LVQLIAGIWE IALENTFGGT ALCSYGGFWL SFAAIYIPWF GILEAYEDNE
SDLNNALGFY LLGWAIFTFG LTVCTMKSTV MFFLLFFLLA LTFLLLSIGH FANRLGVTRA
GGVLGVVVAF IAWYNAYAGV ATKQNSYVLA RPFPLPSTER VIF
