ID   AEE21_ARATH             Reviewed;         546 AA.
AC   Q9SFW5; Q9SGQ5;
DT   22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=Probable acyl-activating enzyme 21;
DE            EC=6.2.1.-;
GN   Name=AEE21; OrderedLocusNames=At1g76290; ORFNames=F15M4.21, T23E18.22;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENE FAMILY.
RX   PubMed=12805634; DOI=10.1104/pp.103.020552;
RA   Shockey J.M., Fulda M.S., Browse J.;
RT   "Arabidopsis contains a large superfamily of acyl-activating enzymes.
RT   Phylogenetic and biochemical analysis reveals a new class of acyl-coenzyme
RT   a synthetases.";
RL   Plant Physiol. 132:1065-1076(2003).
CC   -!- FUNCTION: May act as an acid--thiol ligase that activates carboxylic
CC       acids by forming acyl-CoAs. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAF17636.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC009978; AAF17636.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AC012394; AAF16671.1; -; Genomic_DNA.
DR   EMBL; CP002684; AEE35821.1; -; Genomic_DNA.
DR   PIR; D96790; D96790.
DR   RefSeq; NP_177756.1; NM_106279.2.
DR   AlphaFoldDB; Q9SFW5; -.
DR   SMR; Q9SFW5; -.
DR   STRING; 3702.AT1G76290.1; -.
DR   iPTMnet; Q9SFW5; -.
DR   PaxDb; Q9SFW5; -.
DR   PRIDE; Q9SFW5; -.
DR   ProteomicsDB; 244744; -.
DR   EnsemblPlants; AT1G76290.1; AT1G76290.1; AT1G76290.
DR   GeneID; 843962; -.
DR   Gramene; AT1G76290.1; AT1G76290.1; AT1G76290.
DR   KEGG; ath:AT1G76290; -.
DR   Araport; AT1G76290; -.
DR   TAIR; locus:2014599; AT1G76290.
DR   eggNOG; KOG1176; Eukaryota.
DR   HOGENOM; CLU_000022_59_5_1; -.
DR   InParanoid; Q9SFW5; -.
DR   OMA; VCLREVN; -.
DR   OrthoDB; 312083at2759; -.
DR   PhylomeDB; Q9SFW5; -.
DR   BioCyc; ARA:AT1G76290-MON; -.
DR   PRO; PR:Q9SFW5; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q9SFW5; baseline and differential.
DR   Genevisible; Q9SFW5; AT.
DR   GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006631; P:fatty acid metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.300.30; -; 1.
DR   Gene3D; 3.40.50.12780; -; 1.
DR   InterPro; IPR025110; AMP-bd_C.
DR   InterPro; IPR045851; AMP-bd_C_sf.
DR   InterPro; IPR020845; AMP-binding_CS.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig.
DR   InterPro; IPR042099; ANL_N_sf.
DR   Pfam; PF00501; AMP-binding; 1.
DR   Pfam; PF13193; AMP-binding_C; 1.
DR   PROSITE; PS00455; AMP_BINDING; 1.
PE   3: Inferred from homology;
KW   Fatty acid metabolism; Ligase; Lipid metabolism; Reference proteome.
FT   CHAIN           1..546
FT                   /note="Probable acyl-activating enzyme 21"
FT                   /id="PRO_0000415731"
SQ   SEQUENCE   546 AA;  60494 MW;  3CBA5944955342AB CRC64;
     MEGTMRCSAN YVPLSPISFL ERAAVVFGSR TSVVYGDIQY TWHQTRDRCV RLASALSDLG
     LSRHDVVAAL APNVPALCEL YFGAPMAGAV LCVLNTTFDS QMLAMALEKT KPKVFFVDSE
     FLSVAEESLS LLSNIEEKPL IITITENPTE QSKYEQYEDF LSTGNPNFKP IRPVDECDPI
     ALNFTSGTTS TPKCVVYSHR GAYLNATAVG VMNEMKPMPV YLCTVPMYHC SGWCYIWTVT
     AFGGVIVCLR EVNDEVIFDS IVKHKVTNFG GSPPVLNMIA NARDSVKKSF PWTVQVMSGG
     SSPPEVMLKL KKLGFKVMMA YGCSEVYGLG TACLWMPEWE TLPEEESLRL KARDGLNHFA
     KEAVDVLDPT TMKSVPHDGK TIRVIALRGN TVMSGYFKDK EATEAAFRGG WYWSRDMGVI
     DPDGYIQFKD RSQDVITCGG EIVGSKEIEG ILYSHPAVYD AGVVGRPDET LGESMCAFVK
     LKEGAEAREE EIIEFCKRKL GNKNMKMIPK TVVFSDVPKT PTGKIRKNVL RKMAKDMGYV
     QLRAVE