AEF1_DROME
ID AEF1_DROME Reviewed; 308 AA.
AC P39413; Q0E8C4; Q9VP37;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 172.
DE RecName: Full=Adult enhancer factor 1;
DE Short=AEF-1;
GN Name=Aef1; ORFNames=CG5683;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC TISSUE=Ovary;
RX PubMed=1508206; DOI=10.1128/mcb.12.9.4093-4103.1992;
RA Falb D., Maniatis T.;
RT "Drosophila transcriptional repressor protein that binds specifically to
RT negative control elements in fat body enhancers.";
RL Mol. Cell. Biol. 12:4093-4103(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Transcriptional repressor that binds specifically to fat
CC body-specific enhancers, namely the adult ADH enhancer (AAE) and the
CC enhancer that controls yolk protein gene expression.
CC {ECO:0000269|PubMed:1508206}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:1508206}.
CC -!- TISSUE SPECIFICITY: Found in all tissues examined including the ovary
CC and the fat body. {ECO:0000269|PubMed:1508206}.
CC -!- DEVELOPMENTAL STAGE: Higher levels are found in third-instar larvae and
CC in adults. {ECO:0000269|PubMed:1508206}.
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DR EMBL; M90755; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AE014296; AAF51721.1; -; Genomic_DNA.
DR EMBL; AY060435; AAL25474.1; -; mRNA.
DR PIR; A44496; A44496.
DR RefSeq; NP_524200.1; NM_079476.3.
DR RefSeq; NP_730630.1; NM_168903.3.
DR RefSeq; NP_730631.1; NM_168904.3.
DR AlphaFoldDB; P39413; -.
DR SMR; P39413; -.
DR BioGRID; 65617; 6.
DR DIP; DIP-22241N; -.
DR IntAct; P39413; 4.
DR STRING; 7227.FBpp0078005; -.
DR PaxDb; P39413; -.
DR DNASU; 40370; -.
DR EnsemblMetazoa; FBtr0078348; FBpp0078004; FBgn0005694.
DR EnsemblMetazoa; FBtr0078349; FBpp0078005; FBgn0005694.
DR EnsemblMetazoa; FBtr0078350; FBpp0078006; FBgn0005694.
DR GeneID; 40370; -.
DR KEGG; dme:Dmel_CG5683; -.
DR CTD; 40370; -.
DR FlyBase; FBgn0005694; Aef1.
DR VEuPathDB; VectorBase:FBgn0005694; -.
DR eggNOG; KOG1721; Eukaryota.
DR HOGENOM; CLU_048648_0_0_1; -.
DR InParanoid; P39413; -.
DR OMA; EAPKRCA; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; P39413; -.
DR SignaLink; P39413; -.
DR BioGRID-ORCS; 40370; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 40370; -.
DR PRO; PR:P39413; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0005694; Expressed in brain and 29 other tissues.
DR ExpressionAtlas; P39413; baseline and differential.
DR Genevisible; P39413; DM.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 4.
DR SMART; SM00355; ZnF_C2H2; 4.
DR SUPFAM; SSF57667; SSF57667; 2.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 4.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 4.
PE 2: Evidence at transcript level;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat; Repressor;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..308
FT /note="Adult enhancer factor 1"
FT /id="PRO_0000046908"
FT ZN_FING 184..206
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 212..234
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 240..262
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 268..290
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 50..94
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 123..143
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 50..78
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 129..143
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 308 AA; 33782 MW; 54F04D90B6FC4250 CRC64;
MMHIKSLPHA HAAATAMSSN CDIVIVAAQP QTTIANNNNN ETVTQATHPA HMAAVQQQQQ
QQQQQQQQHH QQQQQQSSGP PSVPPPPTEL PLPFQMHLSG ISAEAHSAAQ AAAMAAAQAA
AAQAAAAEQQ QPPPPTSHLT HLTTHSPTTI HSEHYLANGH SEHPGEGNAA VGVGGAVREP
EKPFHCTVCD RRFRQLSTLT NHVKIHTGEK PYKCNVCDKT FRQSSTLTNH LKIHTGEKPY
NCNFCPKHFR QLSTLANHVK IHTGEKPFEC VICKKQFRQS STLNNHIKIH VMDKVYVPVK
IKTEEDEG
