EFG_VIBVU
ID EFG_VIBVU Reviewed; 699 AA.
AC Q8DCQ8;
DT 04-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Elongation factor G {ECO:0000255|HAMAP-Rule:MF_00054};
DE Short=EF-G {ECO:0000255|HAMAP-Rule:MF_00054};
GN Name=fusA {ECO:0000255|HAMAP-Rule:MF_00054}; OrderedLocusNames=VV1_1338;
OS Vibrio vulnificus (strain CMCP6).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=216895;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CMCP6;
RA Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E.;
RT "Complete genome sequence of Vibrio vulnificus CMCP6.";
RL Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the GTP-dependent ribosomal translocation step
CC during translation elongation. During this step, the ribosome changes
CC from the pre-translocational (PRE) to the post-translocational (POST)
CC state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound
CC deacylated tRNA move to the P and E sites, respectively. Catalyzes the
CC coordinated movement of the two tRNA molecules, the mRNA and
CC conformational changes in the ribosome. {ECO:0000255|HAMAP-
CC Rule:MF_00054}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00054}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. EF-G/EF-2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00054}.
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DR EMBL; AE016795; AAO09792.1; -; Genomic_DNA.
DR RefSeq; WP_011079317.1; NC_004459.3.
DR PDB; 5TV2; X-ray; 1.60 A; A=1-405.
DR PDBsum; 5TV2; -.
DR AlphaFoldDB; Q8DCQ8; -.
DR SMR; Q8DCQ8; -.
DR PRIDE; Q8DCQ8; -.
DR EnsemblBacteria; AAO09792; AAO09792; VV1_1338.
DR KEGG; vvu:VV1_1338; -.
DR PATRIC; fig|196600.6.peg.3007; -.
DR HOGENOM; CLU_002794_4_1_6; -.
DR OMA; AATTCHW; -.
DR Proteomes; UP000002275; Chromosome 1.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-UniRule.
DR CDD; cd16262; EFG_III; 1.
DR CDD; cd01434; EFG_mtEFG1_IV; 1.
DR CDD; cd03713; EFG_mtEFG_C; 1.
DR Gene3D; 3.30.230.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00054_B; EF_G_EF_2_B; 1.
DR InterPro; IPR041095; EFG_II.
DR InterPro; IPR009022; EFG_III.
DR InterPro; IPR035647; EFG_III/V.
DR InterPro; IPR035649; EFG_V.
DR InterPro; IPR000640; EFG_V-like.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR031157; G_TR_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR InterPro; IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR InterPro; IPR004540; Transl_elong_EFG/EF2.
DR InterPro; IPR005517; Transl_elong_EFG/EF2_IV.
DR Pfam; PF00679; EFG_C; 1.
DR Pfam; PF14492; EFG_III; 1.
DR Pfam; PF03764; EFG_IV; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SMART; SM00838; EFG_C; 1.
DR SMART; SM00889; EFG_IV; 1.
DR SUPFAM; SSF50447; SSF50447; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF54211; SSF54211; 1.
DR SUPFAM; SSF54980; SSF54980; 2.
DR TIGRFAMs; TIGR00484; EF-G; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS00301; G_TR_1; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Elongation factor; GTP-binding;
KW Nucleotide-binding; Protein biosynthesis.
FT CHAIN 1..699
FT /note="Elongation factor G"
FT /id="PRO_0000091263"
FT DOMAIN 8..290
FT /note="tr-type G"
FT BINDING 17..24
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00054"
FT BINDING 88..92
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00054"
FT BINDING 142..145
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00054"
FT HELIX 7..9
FT /evidence="ECO:0007829|PDB:5TV2"
FT STRAND 10..16
FT /evidence="ECO:0007829|PDB:5TV2"
FT HELIX 24..37
FT /evidence="ECO:0007829|PDB:5TV2"
FT STRAND 66..72
FT /evidence="ECO:0007829|PDB:5TV2"
FT STRAND 82..87
FT /evidence="ECO:0007829|PDB:5TV2"
FT HELIX 99..105
FT /evidence="ECO:0007829|PDB:5TV2"
FT STRAND 107..114
FT /evidence="ECO:0007829|PDB:5TV2"
FT TURN 115..117
FT /evidence="ECO:0007829|PDB:5TV2"
FT HELIX 124..132
FT /evidence="ECO:0007829|PDB:5TV2"
FT STRAND 137..142
FT /evidence="ECO:0007829|PDB:5TV2"
FT HELIX 151..162
FT /evidence="ECO:0007829|PDB:5TV2"
FT STRAND 166..174
FT /evidence="ECO:0007829|PDB:5TV2"
FT HELIX 176..178
FT /evidence="ECO:0007829|PDB:5TV2"
FT STRAND 181..184
FT /evidence="ECO:0007829|PDB:5TV2"
FT TURN 185..188
FT /evidence="ECO:0007829|PDB:5TV2"
FT STRAND 189..193
FT /evidence="ECO:0007829|PDB:5TV2"
FT TURN 195..198
FT /evidence="ECO:0007829|PDB:5TV2"
FT STRAND 199..205
FT /evidence="ECO:0007829|PDB:5TV2"
FT HELIX 209..211
FT /evidence="ECO:0007829|PDB:5TV2"
FT HELIX 212..227
FT /evidence="ECO:0007829|PDB:5TV2"
FT HELIX 231..240
FT /evidence="ECO:0007829|PDB:5TV2"
FT HELIX 245..257
FT /evidence="ECO:0007829|PDB:5TV2"
FT STRAND 262..266
FT /evidence="ECO:0007829|PDB:5TV2"
FT TURN 269..272
FT /evidence="ECO:0007829|PDB:5TV2"
FT HELIX 275..285
FT /evidence="ECO:0007829|PDB:5TV2"
FT STRAND 295..298
FT /evidence="ECO:0007829|PDB:5TV2"
FT STRAND 304..307
FT /evidence="ECO:0007829|PDB:5TV2"
FT STRAND 316..325
FT /evidence="ECO:0007829|PDB:5TV2"
FT TURN 326..328
FT /evidence="ECO:0007829|PDB:5TV2"
FT STRAND 329..342
FT /evidence="ECO:0007829|PDB:5TV2"
FT STRAND 346..349
FT /evidence="ECO:0007829|PDB:5TV2"
FT TURN 350..353
FT /evidence="ECO:0007829|PDB:5TV2"
FT STRAND 354..357
FT /evidence="ECO:0007829|PDB:5TV2"
FT STRAND 361..367
FT /evidence="ECO:0007829|PDB:5TV2"
FT STRAND 369..376
FT /evidence="ECO:0007829|PDB:5TV2"
FT STRAND 380..385
FT /evidence="ECO:0007829|PDB:5TV2"
FT STRAND 394..396
FT /evidence="ECO:0007829|PDB:5TV2"
SQ SEQUENCE 699 AA; 77413 MW; EC17B5350DDCC311 CRC64;
MARKTPIERY RNIGICAHVD AGKTTTTERI LFYTGLSHKI GEVHDGAATM DWMEQEQERG
ITITSAATTT FWRGMEAQFQ DHRVNIIDTP GHVDFTIEVE RSLRVLDGAV VVFCGSSGVE
PQSETVWRQA DKYHVPRMVF VNKMDRAGAD FLRVVDQIKN RLGANPVPIQ LNVGAEEDFK
GVIDLIKMKM INWNEADQGM TFTYEEIPAD MIELAEEWRN NLVEAAAEAS EELMDKYLEE
GELTEAEIKQ ALRARTLNNE IVLATCGSAF KNKGVQAVLD AVIEYLPSPI DVPAIKGIDE
NDNEVERHAD DNEPFSALAF KIATDPFVGT LTFIRVYSGV VNTGDAVYNS VKQKKERFGR
IVQMHANKRE EIKEVRAGDI AAAIGLKDVT TGDTLCNSDH KVILERMEFP EPVIQIAVEP
RSKADQEKMG IALGKLAAED PSFRVETDAE TGQTLISGMG ELHLDIIVDR MKREFSVDCN
VGKPQVAYRE TIRGKSEVEG KFVRQSGGRG QYGHVWIKLE PSEPGAGFVF VDEVVGGVIP
KEYISSVAKG IEEQMNSGVL AGYPVLDIKA TLFDGSYHDV DSSEMAFKIA GSMAFKKGAL
EAQPVILEPM MKVEVTTPED WMGDVVGDLN RRRGIIEGMD EGVAGLKIIR AQVPLSEMFG
YATDLRSATQ GRASYSMEFF EYAEVPKNIA EAIVAERGY
