EFHB_BOVIN
ID EFHB_BOVIN Reviewed; 877 AA.
AC F1MMV1;
DT 03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT 10-APR-2019, sequence version 3.
DT 03-AUG-2022, entry version 54.
DE RecName: Full=EF-hand domain-containing family member B;
DE AltName: Full=Cilia- and flagella-associated protein 21 {ECO:0000303|PubMed:34715025};
GN Name=EFHB {ECO:0000312|VGNC:VGNC:28350};
GN Synonyms=CFAP21 {ECO:0000303|PubMed:34715025};
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913 {ECO:0000312|Proteomes:UP000009136};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Hereford;
RX PubMed=19393038; DOI=10.1186/gb-2009-10-4-r42;
RA Zimin A.V., Delcher A.L., Florea L., Kelley D.R., Schatz M.C., Puiu D.,
RA Hanrahan F., Pertea G., Van Tassell C.P., Sonstegard T.S., Marcais G.,
RA Roberts M., Subramanian P., Yorke J.A., Salzberg S.L.;
RT "A whole-genome assembly of the domestic cow, Bos taurus.";
RL Genome Biol. 10:R42.01-R42.10(2009).
RN [2] {ECO:0007744|PDB:7RRO}
RP STRUCTURE BY ELECTRON MICROSCOPY (3.40 ANGSTROMS), FUNCTION, SUBCELLULAR
RP LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=34715025; DOI=10.1016/j.cell.2021.10.007;
RA Gui M., Farley H., Anujan P., Anderson J.R., Maxwell D.W., Whitchurch J.B.,
RA Botsch J.J., Qiu T., Meleppattu S., Singh S.K., Zhang Q., Thompson J.,
RA Lucas J.S., Bingle C.D., Norris D.P., Roy S., Brown A.;
RT "De novo identification of mammalian ciliary motility proteins using cryo-
RT EM.";
RL Cell 184:5791-5806.e19(2021).
CC -!- FUNCTION: Cytosolic sensor for calcium, modulates the interaction of
CC STIM1 and ORAI1 upon store depletion and the activation of store-
CC operated Ca(2+) entry (SOCE) and NFAT translocation from cytosol to
CC nucleus (By similarity). Microtubule inner protein (MIP) part of the
CC dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is
CC required for motile cilia beating (PubMed:34715025).
CC {ECO:0000250|UniProtKB:Q8N7U6, ECO:0000269|PubMed:34715025}.
CC -!- SUBUNIT: Interacts with STIM1 and ORAI1; the interactions take place
CC upon Ca(2+)-store depletion and dissociate through a Ca(2+)-dependent
CC mechanism. Interaction with STIM1 inhibits STIM1 interaction with
CC SARAF. {ECO:0000250|UniProtKB:Q8N7U6}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q8N7U6}.
CC Cytoplasm, cytoskeleton, cilium axoneme {ECO:0000269|PubMed:34715025}.
CC -!- TISSUE SPECIFICITY: Expressed in trachea multiciliated cells.
CC {ECO:0000269|PubMed:34715025}.
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DR PDB; 7RRO; EM; 3.40 A; 1/2=1-877.
DR PDBsum; 7RRO; -.
DR STRING; 9913.ENSBTAP00000053164; -.
DR PaxDb; F1MMV1; -.
DR Ensembl; ENSBTAT00000061194.3; ENSBTAP00000053164.3; ENSBTAG00000013806.7.
DR VEuPathDB; HostDB:ENSBTAG00000013806; -.
DR VGNC; VGNC:28350; EFHB.
DR eggNOG; ENOG502QV2M; Eukaryota.
DR GeneTree; ENSGT00530000063528; -.
DR HOGENOM; CLU_017580_0_0_1; -.
DR InParanoid; F1MMV1; -.
DR OMA; WAPLGKS; -.
DR TreeFam; TF323832; -.
DR Proteomes; UP000009136; Chromosome 1.
DR Bgee; ENSBTAG00000013806; Expressed in spermatid and 108 other tissues.
DR GO; GO:0005879; C:axonemal microtubule; IDA:UniProtKB.
DR GO; GO:0061891; F:calcium ion sensor activity; IEA:Ensembl.
DR GO; GO:0032091; P:negative regulation of protein binding; IEA:Ensembl.
DR GO; GO:0070884; P:regulation of calcineurin-NFAT signaling cascade; IEA:Ensembl.
DR GO; GO:2001256; P:regulation of store-operated calcium entry; IEA:Ensembl.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR040193; EFHC1/EFHC2/EFHB.
DR PANTHER; PTHR12086; PTHR12086; 1.
DR Pfam; PF13499; EF-hand_7; 1.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Calcium; Calcium transport; Cell projection; Cytoplasm;
KW Cytoskeleton; Ion transport; Metal-binding; Reference proteome; Repeat;
KW Transport.
FT CHAIN 1..877
FT /note="EF-hand domain-containing family member B"
FT /id="PRO_0000456159"
FT DOMAIN 605..640
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 641..676
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 618
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q8N7U6"
FT BINDING 622
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q8N7U6"
FT BINDING 629
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q8N7U6"
FT BINDING 654
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 656
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 658
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 665
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
SQ SEQUENCE 877 AA; 97819 MW; 87A415C9DDA47CEF CRC64;
MCSFLRVPSP KGLEPSKGLG APLQPGTAYP SEMSALGPGM TVSGRKSPLS MEVGLAPEGK
DDLEERRVFM GTTSPTELGL RVGLRKDFPC SKTYEERMAS PEIRSPSSKG LELRLKRQNI
SRTVMDGSNL GVHRVSASQE TKPPLSALPG RVGLEKERFL AGYCPGRVTQ PPLGQGCESP
QASGGRRCPV TGDPEGFGAS VSKLPALGME CRGELGGESA CVVMKPRAET EPPVEVDMGL
TRLEEPAEME SPEPQMGLVM EPPAWQLAQQ PEEQREAENT EPGVEPPDRI RPIYSGKFFD
RMPCWPSAGK VLPIGYRAAT CLTERFPRLM TPPEAKKFFN FRYPPAGAER VFYGRANDPQ
IAPSLTHGIR SKISIPAKVL INPQPITTFQ QKMKDKKESV YFSNQRAPLG KSHDQTPGLP
KGLDILNTTF GTAIVRETSA RDMVNPPKPY KEVFEEAQAG HDLYVVSHND YFVGEAKNRK
YDPSSFHRFN LYGIPTPHFN DGRNMAKTLH WLHELQMKRG AKIVSKRVDD FKEKFQHRLG
RVLDPIAETM NVPPDYTFGA FLRPEDYGVG DLIHRRLPGE YLRGKDRQRG LVAAVRHHLK
KVNYQNFDTL LAAFRHYDKK GDGVIDRAEL QEACDQACLH LDEKLLDQLF EYCDVDKDGL
INYLEFANFL TWKDKTPLKE YEERVLIKGR KADCANPAEA NVEESEPALL LKPEDIVLKE
PGSSEKTLRT LLRPSDKVSN HYKTTSSEIS AVVGAVPSTC YPTYGVPTIR SDIPAPLIRR
VSDRTSYGEE GNAYSLLHPT IFAQKGVFER DFFKTRSKQE ISEILCNIGV KLSEDEFENV
WNLASKKHHR GEVCVENIRS VLDELQHADG AKCKAAT
