EFHB_MOUSE
ID EFHB_MOUSE Reviewed; 853 AA.
AC Q8CDU5; A2RSG9;
DT 03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=EF-hand domain-containing family member B {ECO:0000305};
DE AltName: Full=Cilia- and flagella-associated protein 21;
GN Name=Efhb {ECO:0000312|MGI:MGI:3045296}; Synonyms=Cfap21;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain, and Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Cytosolic sensor for calcium, modulates the interaction of
CC STIM1 and ORAI1 upon store depletion and the activation of store-
CC operated Ca(2+) entry (SOCE) and NFAT translocation from cytosol to
CC nucleus (By similarity). Microtubule inner protein (MIP) part of the
CC dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is
CC required for motile cilia beating (By similarity).
CC {ECO:0000250|UniProtKB:F1MMV1, ECO:0000250|UniProtKB:Q8N7U6}.
CC -!- SUBUNIT: Interacts with STIM1 and ORAI1; the interactions take place
CC upon Ca(2+)-store depletion and dissociate through a Ca(2+)-dependent
CC mechanism. Interaction with STIM1 inhibits STIM1 interaction with
CC SARAF. {ECO:0000250|UniProtKB:Q8N7U6}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q8N7U6}.
CC Cytoplasm, cytoskeleton, cilium axoneme {ECO:0000250|UniProtKB:F1MMV1}.
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DR EMBL; AK029550; BAC26511.1; -; mRNA.
DR EMBL; CH466559; EDL23660.1; -; Genomic_DNA.
DR EMBL; BC132103; AAI32104.1; -; mRNA.
DR EMBL; BC137880; AAI37881.1; -; mRNA.
DR CCDS; CCDS37652.1; -.
DR RefSeq; NP_766085.2; NM_172497.3.
DR AlphaFoldDB; Q8CDU5; -.
DR SMR; Q8CDU5; -.
DR STRING; 10090.ENSMUSP00000024725; -.
DR iPTMnet; Q8CDU5; -.
DR PhosphoSitePlus; Q8CDU5; -.
DR MaxQB; Q8CDU5; -.
DR PaxDb; Q8CDU5; -.
DR PRIDE; Q8CDU5; -.
DR ProteomicsDB; 275439; -.
DR Antibodypedia; 27043; 109 antibodies from 17 providers.
DR DNASU; 211482; -.
DR Ensembl; ENSMUST00000024725; ENSMUSP00000024725; ENSMUSG00000023931.
DR GeneID; 211482; -.
DR KEGG; mmu:211482; -.
DR UCSC; uc008czl.1; mouse.
DR CTD; 151651; -.
DR MGI; MGI:3045296; Efhb.
DR VEuPathDB; HostDB:ENSMUSG00000023931; -.
DR eggNOG; ENOG502QV2M; Eukaryota.
DR GeneTree; ENSGT00530000063528; -.
DR HOGENOM; CLU_017580_0_0_1; -.
DR InParanoid; Q8CDU5; -.
DR OMA; WAPLGKS; -.
DR OrthoDB; 1016066at2759; -.
DR PhylomeDB; Q8CDU5; -.
DR TreeFam; TF323832; -.
DR BioGRID-ORCS; 211482; 0 hits in 71 CRISPR screens.
DR ChiTaRS; Efhb; mouse.
DR PRO; PR:Q8CDU5; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q8CDU5; protein.
DR Bgee; ENSMUSG00000023931; Expressed in spermatid and 56 other tissues.
DR ExpressionAtlas; Q8CDU5; baseline and differential.
DR Genevisible; Q8CDU5; MM.
DR GO; GO:0005879; C:axonemal microtubule; ISS:UniProtKB.
DR GO; GO:0061891; F:calcium ion sensor activity; ISS:UniProtKB.
DR GO; GO:0032091; P:negative regulation of protein binding; ISS:UniProtKB.
DR GO; GO:0070884; P:regulation of calcineurin-NFAT signaling cascade; ISS:UniProtKB.
DR GO; GO:2001256; P:regulation of store-operated calcium entry; ISS:UniProtKB.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR040193; EFHC1/EFHC2/EFHB.
DR PANTHER; PTHR12086; PTHR12086; 1.
DR Pfam; PF13499; EF-hand_7; 1.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 2.
PE 1: Evidence at protein level;
KW Calcium; Cell projection; Cytoplasm; Cytoskeleton; Metal-binding;
KW Reference proteome; Repeat.
FT CHAIN 1..853
FT /note="EF-hand domain-containing family member B"
FT /id="PRO_0000252095"
FT DOMAIN 581..616
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 617..652
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 244..266
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 9..23
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 594
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000305"
FT BINDING 598
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000305"
FT BINDING 605
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000305"
FT BINDING 630
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 632
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 634
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 641
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT CONFLICT 632
FT /note="D -> G (in Ref. 1; BAC26511)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 853 AA; 95743 MW; 9051B1D2F35AA68E CRC64;
MCSFVRVGSP KPLQTSASPL EMSSLRRTRA PEISELGLTP EQKDDIRDRV LRGSKSPTEL
GFDLRLEQDR KWRERMGSSE AKSPPCHALG VGLERHTISG TPTEMGNLGL HKGSAFQGSK
PLGVLPGRVG PENKGLPPRL RYGGTLHPPF STVHASPLAA ESRRRPLAWG SAWTDAVVEK
QPVVGLELRK EPEKEPTCVV MNPYPEMPPK EVDIGLPQTQ ESDEAKNTEP LIGLVREPSE
CPFAQQPEEK KEPGSTEPGV EPPGNIRPIY SGKFFDRVPC WPSAGKVKPV GYRVATCLTE
KLPRLMTPPE AKKYFNFRYP PAGAERVFYG RANDPQIAPY LTHGLRSKIS IPMGSLINPQ
PITTFQQKIK DKKESIYFSH QRAPLGKSHD QTPGLPKGMD VINTTLGTPT IRELSVRDTV
NPSKSFEDVL KEGQEGHDLY TVSHNDYFAG EAKNRKYNPA SFHRFNLYGI PTPHFNDGRT
MAKALHWLHE LQMERGAKIV SKRVDDFKEK FQHKLGKVLD PIAETMNVPP GHTFGSCLHP
EEYGAGDLIH YRSPDEYLRG KDHQRAVVAA ARHHLKKFNH QNFDTLQVAF RHYDKKGDGV
IDRAELHEAC VQANLHLDKM LLDHLFDYCD VDQDGLINYL EFANFLNWKD RIPLKEHEKR
VVVKGKKPDC ENVTDTSMGE AEPSLLINPE DIVPKEPGSS EETLRTIQRP GDKVSHQYKT
TSSEINAVVG AVPSMCHPIF GVPTIRSDIS APRIRRVSDM NNYGDEGNAY SLLHPSIFSQ
KGVFERDFFK TRSKEEISDI LTNIGVKLSK EEFENVWNLA SKKHQRGEVC VETIRNVLDE
LLHADLVKCK TAM
