EFL2_CAEEL
ID EFL2_CAEEL Reviewed; 358 AA.
AC A5HWA8; A5HWA6; Q9BIE8;
DT 07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT 26-JUN-2007, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Transcription factor efl-2 {ECO:0000305};
DE AltName: Full=E2F-like family member 2 {ECO:0000312|WormBase:Y48C3A.17d};
GN Name=efl-2 {ECO:0000312|WormBase:Y48C3A.17d};
GN ORFNames=Y48C3A.17 {ECO:0000312|WormBase:Y48C3A.17d};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|EMBL:AAK19023.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
RX PubMed=11463372; DOI=10.1016/s1097-2765(01)00194-0;
RA Ceol C.J., Horvitz H.R.;
RT "dpl-1 DP and efl-1 E2F act with lin-35 Rb to antagonize Ras signaling in
RT C.elegans vulval development.";
RL Mol. Cell 7:461-473(2001).
RN [2] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=17881492; DOI=10.1242/dev.004606;
RA Schertel C., Conradt B.;
RT "C. elegans orthologs of components of the RB tumor suppressor complex have
RT distinct pro-apoptotic functions.";
RL Development 134:3691-3701(2007).
CC -!- FUNCTION: Transcription activator (By similarity). May activate
CC expression of the pro-apoptotic genes ced-4 and ced-3
CC (PubMed:17881492). Required for constitutive, and for DNA damage-
CC induced, programmed cell death of germ cells (PubMed:17881492).
CC {ECO:0000250|UniProtKB:O35261, ECO:0000269|PubMed:17881492}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|RuleBase:RU003796}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=d {ECO:0000312|WormBase:Y48C3A.17d};
CC IsoId=A5HWA8-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:Y48C3A.17b};
CC IsoId=A5HWA8-2; Sequence=VSP_060929, VSP_060930;
CC -!- DISRUPTION PHENOTYPE: Reduced level of constitutive germ cell apoptosis
CC on a ced-6 mutant background, in hermaphrodites (PubMed:17881492).
CC Blocks DNA damage-induced germ cell apoptosis, but does not affect
CC associated cell-cycle arrest (PubMed:17881492).
CC {ECO:0000269|PubMed:17881492}.
CC -!- SIMILARITY: Belongs to the E2F/DP family.
CC {ECO:0000255|RuleBase:RU003796}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK19023.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AY028167; AAK19023.1; ALT_INIT; mRNA.
DR EMBL; BX284602; CAN86629.1; -; Genomic_DNA.
DR EMBL; BX284602; CAN86631.1; -; Genomic_DNA.
DR RefSeq; NP_001122658.1; NM_001129186.1. [A5HWA8-2]
DR RefSeq; NP_001122660.1; NM_001129188.1. [A5HWA8-1]
DR AlphaFoldDB; A5HWA8; -.
DR SMR; A5HWA8; -.
DR ComplexPortal; CPX-1145; E2F2-DP1 transcription factor complex.
DR ComplexPortal; CPX-1146; RB1-E2F2-TFDP1 transcription repressor complex.
DR IntAct; A5HWA8; 8.
DR EPD; A5HWA8; -.
DR EnsemblMetazoa; Y48C3A.17b.1; Y48C3A.17b.1; WBGene00001162. [A5HWA8-2]
DR EnsemblMetazoa; Y48C3A.17d.1; Y48C3A.17d.1; WBGene00001162. [A5HWA8-1]
DR GeneID; 174980; -.
DR KEGG; cel:CELE_Y48C3A.17; -.
DR UCSC; Y48C3A.17a; c. elegans.
DR CTD; 174980; -.
DR WormBase; Y48C3A.17b; CE41054; WBGene00001162; efl-2.
DR WormBase; Y48C3A.17d; CE41056; WBGene00001162; efl-2.
DR GeneTree; ENSGT00940000170364; -.
DR HOGENOM; CLU_055514_0_0_1; -.
DR Reactome; R-CEL-1538133; G0 and Early G1.
DR Reactome; R-CEL-2173796; SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00001162; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR ExpressionAtlas; A5HWA8; baseline and differential.
DR GO; GO:0090575; C:RNA polymerase II transcription regulator complex; IC:ComplexPortal.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0043433; P:negative regulation of DNA-binding transcription factor activity; IC:ComplexPortal.
DR GO; GO:0042981; P:regulation of apoptotic process; IC:ComplexPortal.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IC:ComplexPortal.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR015633; E2F.
DR InterPro; IPR037241; E2F-DP_heterodim.
DR InterPro; IPR003316; E2F_WHTH_DNA-bd_dom.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR12081; PTHR12081; 2.
DR Pfam; PF02319; E2F_TDP; 1.
DR SMART; SM01372; E2F_TDP; 1.
DR SUPFAM; SSF144074; SSF144074; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..358
FT /note="Transcription factor efl-2"
FT /id="PRO_0000452249"
FT DNA_BIND 36..99
FT /evidence="ECO:0000255"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 331..358
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..17
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 274..284
FT /note="ETFVLPPSTSA -> GARNSQNSEKI (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_060929"
FT VAR_SEQ 285..358
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_060930"
SQ SEQUENCE 358 AA; 39759 MW; C1B01ACB88B3329A CRC64;
MDPSSIDLGS IPGGSEQQQH EDGAAAAAGS PKEVFRSQQS LGLITQRFMS LRQRNEVLNL
NEVAKELNIS KRRVYDVINV LEGLGYVEKV EKNNIRWIGD NNNSEEQNAL EARVEMLRQQ
EKLLELMIRD AQAIIELHFE DPIERPYNYV SKEDIRKTAE PNTKSIIVKS ELDTSSSFEI
QVTDPSTSGS HDMIVRNKNG VKSHALLFTN DPPEYGDEED EDDVKKLSSQ KRFETSEALR
VTLAEMLTDP TTISSSPKHI KLEEPEEIDT EDLETFVLPP STSASQSNAP YLPETPGRGL
FFSPFKSLID PCILPSFSDD LTSGYINITT PSRATGAGAG ASGQEDEEPV SVMDFFSD
