EFM5_DROME
ID EFM5_DROME Reviewed; 223 AA.
AC Q9VMH7; Q95SB4;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 2.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Protein-lysine N-methyltransferase CG9154 {ECO:0000255|HAMAP-Rule:MF_03187};
DE EC=2.1.1.- {ECO:0000255|HAMAP-Rule:MF_03187};
GN ORFNames=CG9154;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Ovary;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: S-adenosyl-L-methionine-dependent protein-lysine N-
CC methyltransferase that methylates elongation factor 1-alpha.
CC {ECO:0000255|HAMAP-Rule:MF_03187}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03187}.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. EFM5 family. {ECO:0000255|HAMAP-Rule:MF_03187}.
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DR EMBL; AE014134; AAF52340.2; -; Genomic_DNA.
DR EMBL; AY060876; AAL28424.1; -; mRNA.
DR RefSeq; NP_001285662.1; NM_001298733.1.
DR RefSeq; NP_608993.2; NM_135149.3.
DR AlphaFoldDB; Q9VMH7; -.
DR SMR; Q9VMH7; -.
DR STRING; 7227.FBpp0078844; -.
DR PaxDb; Q9VMH7; -.
DR EnsemblMetazoa; FBtr0079213; FBpp0078844; FBgn0031777.
DR EnsemblMetazoa; FBtr0343128; FBpp0309827; FBgn0031777.
DR GeneID; 33858; -.
DR KEGG; dme:Dmel_CG9154; -.
DR UCSC; CG9154-RA; d. melanogaster.
DR FlyBase; FBgn0031777; CG9154.
DR VEuPathDB; VectorBase:FBgn0031777; -.
DR eggNOG; KOG3350; Eukaryota.
DR GeneTree; ENSGT00390000016366; -.
DR HOGENOM; CLU_074410_2_1_1; -.
DR InParanoid; Q9VMH7; -.
DR OMA; HKCNFRP; -.
DR OrthoDB; 1272987at2759; -.
DR PhylomeDB; Q9VMH7; -.
DR Reactome; R-DME-8876725; Protein methylation.
DR BioGRID-ORCS; 33858; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 33858; -.
DR PRO; PR:Q9VMH7; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0031777; Expressed in wing disc and 25 other tissues.
DR ExpressionAtlas; Q9VMH7; baseline and differential.
DR Genevisible; Q9VMH7; DM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0016279; F:protein-lysine N-methyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0018022; P:peptidyl-lysine methylation; IEA:UniProtKB-UniRule.
DR HAMAP; MF_03187; Methyltr_EFM5; 1.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR019369; Efm5/EEF1AKMT1.
DR InterPro; IPR041370; Mlase_EEF1AKMT1/ZCCHC4.
DR PANTHER; PTHR13200; PTHR13200; 1.
DR Pfam; PF10237; N6-adenineMlase; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Methyltransferase; Reference proteome; Transferase.
FT CHAIN 1..223
FT /note="Protein-lysine N-methyltransferase CG9154"
FT /id="PRO_0000311300"
FT CONFLICT 75
FT /note="E -> K (in Ref. 3; AAL28424)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 223 AA; 26043 MW; 323E36908C5BDE97 CRC64;
MDDDISLPAD TLAILNEFLL ERSKREAEEE NQIANKTGKD AQFEEDWQLS QFWYSTETKH
ALRDVVRKLL AERTEDSGDF SIALLSCPSL YKDIREIHDT VHIFEFDKRF EAYGTDFVHY
DLNCVGSNPD YLKEHHQQYD LIVADPPFLS QECIAKTCEI ITRLQRNQKE SKVILCSGEV
VEPWLTARLP VLKCSFRPEH ERNLGNKFVS YANFNLDEYI ENK
