AEN_MOUSE
ID AEN_MOUSE Reviewed; 336 AA.
AC Q9CZI9; Q3TKV9; Q8BTJ8; Q8C095;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Apoptosis-enhancing nuclease;
DE EC=3.1.-.-;
DE AltName: Full=Interferon-stimulated 20 kDa exonuclease-like 1;
GN Name=Aen; Synonyms=Isg20l1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Salivary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Exonuclease with activity against single- and double-stranded
CC DNA and RNA. Mediates p53-induced apoptosis. When induced by p53
CC following DNA damage, digests double-stranded DNA to form single-
CC stranded DNA and amplifies DNA damage signals, leading to enhancement
CC of apoptosis (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Nucleus, nucleolus
CC {ECO:0000250}. Note=Localized predomintly in the nucleolus.
CC Translocates from the nucleolus to the nucleoplasm upon apoptosis
CC induction (By similarity). {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9CZI9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9CZI9-2; Sequence=VSP_032133;
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC27632.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK090056; BAC41071.1; -; mRNA.
DR EMBL; AK012555; BAB28314.1; -; mRNA.
DR EMBL; AK031972; BAC27632.1; ALT_INIT; mRNA.
DR EMBL; AK165708; BAE38348.1; -; mRNA.
DR EMBL; AK166806; BAE39034.1; -; mRNA.
DR EMBL; AK167042; BAE39210.1; -; mRNA.
DR EMBL; AK167658; BAE39708.1; -; mRNA.
DR EMBL; BC022614; AAH22614.1; -; mRNA.
DR CCDS; CCDS39988.2; -. [Q9CZI9-1]
DR CCDS; CCDS52277.1; -. [Q9CZI9-2]
DR RefSeq; NP_001156411.1; NM_001162939.1. [Q9CZI9-2]
DR RefSeq; NP_080807.3; NM_026531.4. [Q9CZI9-1]
DR RefSeq; XP_006541200.1; XM_006541137.2.
DR AlphaFoldDB; Q9CZI9; -.
DR SMR; Q9CZI9; -.
DR STRING; 10090.ENSMUSP00000103048; -.
DR iPTMnet; Q9CZI9; -.
DR PhosphoSitePlus; Q9CZI9; -.
DR EPD; Q9CZI9; -.
DR MaxQB; Q9CZI9; -.
DR PaxDb; Q9CZI9; -.
DR PeptideAtlas; Q9CZI9; -.
DR PRIDE; Q9CZI9; -.
DR ProteomicsDB; 296073; -. [Q9CZI9-1]
DR ProteomicsDB; 296074; -. [Q9CZI9-2]
DR Antibodypedia; 28501; 86 antibodies from 20 providers.
DR DNASU; 68048; -.
DR Ensembl; ENSMUST00000107421; ENSMUSP00000103044; ENSMUSG00000030609. [Q9CZI9-2]
DR Ensembl; ENSMUST00000107423; ENSMUSP00000103046; ENSMUSG00000030609. [Q9CZI9-1]
DR Ensembl; ENSMUST00000107425; ENSMUSP00000103048; ENSMUSG00000030609. [Q9CZI9-1]
DR GeneID; 68048; -.
DR KEGG; mmu:68048; -.
DR UCSC; uc009hxp.1; mouse. [Q9CZI9-1]
DR CTD; 64782; -.
DR MGI; MGI:1915298; Aen.
DR VEuPathDB; HostDB:ENSMUSG00000030609; -.
DR eggNOG; KOG2249; Eukaryota.
DR GeneTree; ENSGT00940000161660; -.
DR HOGENOM; CLU_022453_0_0_1; -.
DR InParanoid; Q9CZI9; -.
DR OMA; LCPSFTI; -.
DR OrthoDB; 1562214at2759; -.
DR PhylomeDB; Q9CZI9; -.
DR TreeFam; TF354340; -.
DR BioGRID-ORCS; 68048; 10 hits in 73 CRISPR screens.
DR PRO; PR:Q9CZI9; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q9CZI9; protein.
DR Bgee; ENSMUSG00000030609; Expressed in epiblast (generic) and 251 other tissues.
DR ExpressionAtlas; Q9CZI9; baseline and differential.
DR Genevisible; Q9CZI9; MM.
DR GO; GO:0031965; C:nuclear membrane; ISO:MGI.
DR GO; GO:0005730; C:nucleolus; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0004527; F:exonuclease activity; ISS:UniProtKB.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0042771; P:intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; ISS:UniProtKB.
DR GO; GO:0010212; P:response to ionizing radiation; ISS:UniProtKB.
DR Gene3D; 3.30.420.10; -; 1.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00479; EXOIII; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Apoptosis; DNA damage; Exonuclease; Hydrolase;
KW Nuclease; Nucleus; Reference proteome.
FT CHAIN 1..336
FT /note="Apoptosis-enhancing nuclease"
FT /id="PRO_0000324089"
FT DOMAIN 109..265
FT /note="Exonuclease"
FT REGION 1..37
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 56..102
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 276..336
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 25..33
FT /note="Nucleolar localization signal"
FT /evidence="ECO:0000250"
FT MOTIF 164..187
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000250"
FT COMPBIAS 23..37
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 304..318
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..38
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_032133"
FT CONFLICT 62
FT /note="L -> P (in Ref. 1; BAC41071)"
FT /evidence="ECO:0000305"
FT CONFLICT 109
FT /note="C -> R (in Ref. 1; BAC41071)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 336 AA; 37309 MW; 0BE9173A65D7F018 CRC64;
MVAGEVPEST QCPSLTSLNT KDVVRRRHKR RSRQHQRFMA RKALLQEQEL LSTAPGPGLC
LLPSPSQMPA VTEASDSRRQ RPKARSGSNG LCSKKSVPRE APRPGPIKCV AIDCEMVGTG
PQGRVSELAR CSVVSYSGDV LYDKYIRPEM PIVDYRTRWS GITRQHMHKA IPFQVAQKEI
LKLLKGKVVV GHALHNDFQA LKYVHPRSQT RDTTYVPNLL SQPSSLIRTR VSLKDLALNL
LHKKIQVGHQ GHSSVEDAMT AMELYQLVEV QWEQQVASSA QAPAEDRGPD SSTDVEQYMD
DQYWPEDLTQ STGAETNGGP DRQEGEEGQG ARSAPP
