ID   3L22_DEMVE              Reviewed;          88 AA.
AC   A6MFK5;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-JUL-2007, sequence version 1.
DT   25-MAY-2022, entry version 52.
DE   RecName: Full=Long neurotoxin LNTX-2;
DE   Flags: Precursor;
OS   Demansia vestigiata (Lesser black whip snake) (Demansia atra).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Notechinae; Demansia.
OX   NCBI_TaxID=412038;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 34-45, IDENTIFICATION BY
RP   MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX   PubMed=17608513; DOI=10.1021/pr0701613;
RA   St Pierre L., Birrell G.W., Earl S.T.H., Wallis T.P., Gorman J.J.,
RA   de Jersey J., Masci P.P., Lavin M.F.;
RT   "Diversity of toxic components from the venom of the evolutionarily
RT   distinct black whip snake, Demansia vestigiata.";
RL   J. Proteome Res. 6:3093-3107(2007).
CC   -!- FUNCTION: Binds with high affinity to muscular nicotinic acetylcholine
CC       receptors (nAChRs), whereas it binds with a low affinity to neuronal
CC       alpha-7/CHRNA7 nAChRs. {ECO:0000250|UniProtKB:P0C8R6}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17608513}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- MISCELLANEOUS: Has the length of long neurotoxins, but only 4 disulfide
CC       bonds, as short neurotoxins.
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Long-chain
CC       subfamily. Type II alpha-neurotoxin sub-subfamily. {ECO:0000305}.
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DR   EMBL; DQ917516; ABK63545.1; -; mRNA.
DR   AlphaFoldDB; A6MFK5; -.
DR   SMR; A6MFK5; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   CDD; cd00206; snake_toxin; 1.
DR   Gene3D; 2.10.60.10; -; 1.
DR   InterPro; IPR016054; LY6_UPA_recep-like.
DR   InterPro; IPR003571; Snake_3FTx.
DR   InterPro; IPR045860; Snake_toxin-like_sf.
DR   InterPro; IPR018354; Snake_toxin_con_site.
DR   Pfam; PF00021; UPAR_LY6; 1.
DR   SUPFAM; SSF57302; SSF57302; 1.
DR   PROSITE; PS00272; SNAKE_TOXIN; 1.
PE   1: Evidence at protein level;
KW   Acetylcholine receptor inhibiting toxin; Direct protein sequencing;
KW   Disulfide bond; Ion channel impairing toxin; Neurotoxin;
KW   Postsynaptic neurotoxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..88
FT                   /note="Long neurotoxin LNTX-2"
FT                   /id="PRO_5000254109"
FT   DISULFID        24..42
FT                   /evidence="ECO:0000250"
FT   DISULFID        35..63
FT                   /evidence="ECO:0000250"
FT   DISULFID        67..78
FT                   /evidence="ECO:0000250"
FT   DISULFID        79..84
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   88 AA;  9758 MW;  3762D7524AACAC69 CRC64;
     MKTLLLTLVV VTIVCLDFGY ARTCLKTPEV KSEPCPPGQE VCYTKAWRDR MCSFRGKVIE
     LGCAATCPRQ EPGKEITCCS TDDCNTHP