ID   AEN_RAT                 Reviewed;         332 AA.
AC   B2GUW6;
DT   25-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-JUN-2008, sequence version 1.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=Apoptosis-enhancing nuclease {ECO:0000250|UniProtKB:Q8WTP8};
DE            EC=3.1.-.-;
DE   AltName: Full=Interferon-stimulated 20 kDa exonuclease-like 1 {ECO:0000312|RGD:1305051};
GN   Name=Aen {ECO:0000250|UniProtKB:Q8WTP8};
GN   Synonyms=Isg20l1 {ECO:0000312|EMBL:AAI66435.1};
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1] {ECO:0000312|EMBL:EDM08560.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000312|EMBL:AAI66435.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Liver {ECO:0000312|EMBL:AAI66435.1};
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Exonuclease with activity against single- and double-stranded
CC       DNA and RNA. Mediates p53-induced apoptosis. When induced by p53
CC       following DNA damage, digests double-stranded DNA to form single-
CC       stranded DNA and amplifies DNA damage signals, leading to enhancement
CC       of apoptosis (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8WTP8}. Nucleus,
CC       nucleolus {ECO:0000250|UniProtKB:Q8WTP8}. Note=Localized predomintly in
CC       the nucleolus. Translocates from the nucleolus to the nucleoplasm upon
CC       apoptosis induction (By similarity). {ECO:0000250|UniProtKB:Q8WTP8}.
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DR   EMBL; CH473980; EDM08560.1; -; Genomic_DNA.
DR   EMBL; CH473980; EDM08562.1; -; Genomic_DNA.
DR   EMBL; BC166435; AAI66435.1; -; mRNA.
DR   RefSeq; NP_001101957.1; NM_001108487.2.
DR   RefSeq; XP_006229461.1; XM_006229399.3.
DR   AlphaFoldDB; B2GUW6; -.
DR   SMR; B2GUW6; -.
DR   STRING; 10116.ENSRNOP00000062841; -.
DR   PaxDb; B2GUW6; -.
DR   PRIDE; B2GUW6; -.
DR   Ensembl; ENSRNOT00000064438; ENSRNOP00000062841; ENSRNOG00000018421.
DR   GeneID; 361594; -.
DR   KEGG; rno:361594; -.
DR   UCSC; RGD:1305051; rat.
DR   CTD; 64782; -.
DR   RGD; 1305051; Aen.
DR   eggNOG; KOG2249; Eukaryota.
DR   GeneTree; ENSGT00940000161660; -.
DR   HOGENOM; CLU_022453_0_0_1; -.
DR   InParanoid; B2GUW6; -.
DR   OMA; LCPSFTI; -.
DR   OrthoDB; 1562214at2759; -.
DR   PhylomeDB; B2GUW6; -.
DR   PRO; PR:B2GUW6; -.
DR   Proteomes; UP000002494; Chromosome 1.
DR   Proteomes; UP000234681; Chromosome 1.
DR   Bgee; ENSRNOG00000018421; Expressed in pancreas and 20 other tissues.
DR   Genevisible; B2GUW6; RN.
DR   GO; GO:0031965; C:nuclear membrane; IEA:Ensembl.
DR   GO; GO:0005730; C:nucleolus; ISS:UniProtKB.
DR   GO; GO:0005654; C:nucleoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0004527; F:exonuclease activity; ISS:UniProtKB.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0042771; P:intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; ISS:UniProtKB.
DR   GO; GO:0010212; P:response to ionizing radiation; ISS:UniProtKB.
DR   Gene3D; 3.30.420.10; -; 1.
DR   InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF00929; RNase_T; 1.
DR   SMART; SM00479; EXOIII; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
PE   2: Evidence at transcript level;
KW   Apoptosis; DNA damage; Exonuclease; Hydrolase; Nuclease; Nucleus;
KW   Reference proteome.
FT   CHAIN           1..332
FT                   /note="Apoptosis-enhancing nuclease"
FT                   /id="PRO_0000355124"
FT   DOMAIN          105..261
FT                   /note="Exonuclease"
FT                   /evidence="ECO:0000255"
FT   REGION          1..102
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          272..332
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           21..29
FT                   /note="Nucleolar localization signal"
FT                   /evidence="ECO:0000250|UniProtKB:Q8WTP8"
FT   MOTIF           160..183
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000250|UniProtKB:Q8WTP8"
FT   COMPBIAS        18..34
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        307..324
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   332 AA;  37061 MW;  BD5048D7D10C196A CRC64;
     MVPREVPESS LTSLKTKDVA RRRHKRRSRQ HQRFMARKAL LQEQESLSMV PGPGLCPLPS
     PTQTPAGTEA SGNRKQRTKA RSGSKGLCSK RPVPREAPSS GPSKYVAIDC EMVGTGPQGR
     VSELARCSVV SYSGDVLYDK YIRPEMPIVD YRTRWSGITR QHMHKAIPFQ VAQKEILKLL
     KGKVVVGHAL HNDFQALKYV HPGSQIRDTT YVPNLLSQPS SLTRARVSLK DLALNLLHKK
     IQVGHHGHSS VEDAMTAMEL YQLVEVQWEQ QVASTAKAHP EDRGPDSSTD VEQYMDDQYW
     PEDLAQSTRG DTREAQDRQE GEEGQGARSA PP