EFM_ARATH
ID EFM_ARATH Reviewed; 432 AA.
AC Q9ZQ85; Q8W4S3;
DT 06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Myb family transcription factor EFM {ECO:0000303|PubMed:25132385};
DE AltName: Full=MYB-domain transcription factor HHO4 {ECO:0000305};
DE AltName: Full=Protein EARLY FLOWERING MYB {ECO:0000303|PubMed:25132385};
DE AltName: Full=Protein HRS1 HOMOLOG 4 {ECO:0000305};
GN Name=EFM {ECO:0000303|PubMed:25132385}; Synonyms=HHO4 {ECO:0000305};
GN OrderedLocusNames=At2g03500 {ECO:0000312|Araport:AT2G03500};
GN ORFNames=T4M8.7 {ECO:0000312|EMBL:AAL31188.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION,
RP INDUCTION, DEVELOPMENTAL STAGE, INTERACTION WITH JMJ30, AND LACK OF
RP INTERACTION WITH SVP; FLC AND CO.
RX PubMed=25132385; DOI=10.1016/j.devcel.2014.07.004;
RA Yan Y., Shen L., Chen Y., Bao S., Thong Z., Yu H.;
RT "A MYB-domain protein EFM mediates flowering responses to environmental
RT cues in Arabidopsis.";
RL Dev. Cell 30:437-448(2014).
CC -!- FUNCTION: Transcription factor acting as a flowering repressor,
CC directly repressing FT expression in a dosage-dependent manner in the
CC leaf vasculature. {ECO:0000269|PubMed:25132385}.
CC -!- SUBUNIT: Interacts with JMJ30, but not with SVP, FLC or CO.
CC {ECO:0000269|PubMed:25132385}.
CC -!- INTERACTION:
CC Q9ZQ85; Q93Z00: TCP14; NbExp=3; IntAct=EBI-4449948, EBI-4424563;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:25132385}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in vascular tissues of
CC cotyledons, rosette leaves and cauline leaves. Not detected in the
CC vegetative shoot apical meristem. {ECO:0000269|PubMed:25132385}.
CC -!- DEVELOPMENTAL STAGE: Expressed 3 days after germination, with a gradual
CC decrease before the floral transition and remains at low levels
CC afterward. {ECO:0000269|PubMed:25132385}.
CC -!- INDUCTION: Up-regulated by SVP. Down-regulated by high temperature and
CC gibberellic acid treatment. Not regulated by photoperiod, circadian
CC rhythm under long days or vernalization. {ECO:0000269|PubMed:25132385}.
CC -!- DISRUPTION PHENOTYPE: Early flowering under both long-day and short-day
CC conditions. {ECO:0000269|PubMed:25132385}.
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DR EMBL; AC006284; AAD17450.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC05707.1; -; Genomic_DNA.
DR EMBL; AY060559; AAL31188.1; -; mRNA.
DR EMBL; AY133630; AAM91460.1; -; mRNA.
DR EMBL; AK221662; BAD95340.1; -; mRNA.
DR PIR; B84449; B84449.
DR RefSeq; NP_027544.1; NM_126400.3.
DR AlphaFoldDB; Q9ZQ85; -.
DR SMR; Q9ZQ85; -.
DR IntAct; Q9ZQ85; 6.
DR STRING; 3702.AT2G03500.1; -.
DR iPTMnet; Q9ZQ85; -.
DR PaxDb; Q9ZQ85; -.
DR PRIDE; Q9ZQ85; -.
DR ProteomicsDB; 247130; -.
DR EnsemblPlants; AT2G03500.1; AT2G03500.1; AT2G03500.
DR GeneID; 814878; -.
DR Gramene; AT2G03500.1; AT2G03500.1; AT2G03500.
DR KEGG; ath:AT2G03500; -.
DR Araport; AT2G03500; -.
DR TAIR; locus:2063746; AT2G03500.
DR eggNOG; ENOG502QU6F; Eukaryota.
DR HOGENOM; CLU_036551_0_1_1; -.
DR InParanoid; Q9ZQ85; -.
DR OMA; KCSEAEN; -.
DR OrthoDB; 757958at2759; -.
DR PhylomeDB; Q9ZQ85; -.
DR PRO; PR:Q9ZQ85; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9ZQ85; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
DR GO; GO:0009740; P:gibberellic acid mediated signaling pathway; IEP:TAIR.
DR GO; GO:0010452; P:histone H3-K36 methylation; IPI:TAIR.
DR GO; GO:0048579; P:negative regulation of long-day photoperiodism, flowering; IMP:TAIR.
DR GO; GO:1903507; P:negative regulation of nucleic acid-templated transcription; IPI:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR GO; GO:0009266; P:response to temperature stimulus; IEP:TAIR.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR044787; HRS1-like.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR006447; Myb_dom_plants.
DR PANTHER; PTHR31003; PTHR31003; 1.
DR Pfam; PF00249; Myb_DNA-binding; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR TIGRFAMs; TIGR01557; myb_SHAQKYF; 1.
DR PROSITE; PS51294; HTH_MYB; 1.
PE 1: Evidence at protein level;
KW Coiled coil; DNA-binding; Flowering; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..432
FT /note="Myb family transcription factor EFM"
FT /id="PRO_0000436872"
FT DOMAIN 230..290
FT /note="HTH myb-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 261..286
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 123..237
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 354..412
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 36..81
FT /evidence="ECO:0000255"
FT COMPBIAS 123..145
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 183..235
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 368..397
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 432 AA; 47962 MW; 4B2F09FE1C9AF658 CRC64;
MASSSELSLD CKPQSYSMLL KSFGDNFQSD PTTHKLEDLL SRLEQERLKI DAFKRELPLC
MQLLNNAVEV YKQQLEAYRA NSNNNNQSVG TRPVLEEFIP LRNQPEKTNN KGSNWMTTAQ
LWSQSETKPK NIDSTTDQSL PKDEINSSPK LGHFDAKQRN GSGAFLPFSK EQSLPELALS
TEVKRVSPTN EHTNGQDGND ESMINNDNNY NNNNNNNSNS NGVSSTTSQS NRKARRCWSP
DLHRRFVQAL QMLGGSQVAT PKQIRELMKV DGLTNDEVKS HLQKYRLHTR RPSPSPQTSG
GPGPHLVVLG GIWVPPEYTS AHGGTPTLYH HQVHHHHTNT AGPPPPHFCS SQEFYTTPPP
PQPLHHHHFQ TFNGSSGGTA STDSTHHQVT DSPTVEGKSP ESGGGERKGL AALREECEDH
SNINGSEITL KF
