EFNMT_DROME
ID EFNMT_DROME Reviewed; 673 AA.
AC Q9VIK9;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=eEF1A lysine and N-terminal methyltransferase homolog {ECO:0000305};
DE Includes:
DE RecName: Full=eEF1A lysine methyltransferase homolog {ECO:0000305};
DE EC=2.1.1.- {ECO:0000250|UniProtKB:Q8N6R0};
DE Includes:
DE RecName: Full=eEF1A N-terminal methyltransferase homolog {ECO:0000305};
DE EC=2.1.1.- {ECO:0000250|UniProtKB:Q8N6R0};
GN ORFNames=CG2614;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Dual methyltransferase. It catalyzes N-terminal methylation
CC of target proteins via its C-terminus. It catalyzes dimethylation on
CC lysine residues of target proteins via its N-terminus.
CC {ECO:0000250|UniProtKB:Q8N6R0}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-lysyl-[protein] + S-adenosyl-L-methionine = H(+) + N(6)-
CC methyl-L-lysyl-[protein] + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:51736, Rhea:RHEA-COMP:9752, Rhea:RHEA-COMP:13053,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:29969, ChEBI:CHEBI:57856,
CC ChEBI:CHEBI:59789, ChEBI:CHEBI:61929;
CC Evidence={ECO:0000250|UniProtKB:Q8N6R0};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=N(6)-methyl-L-lysyl-[protein] + S-adenosyl-L-methionine = H(+)
CC + N(6),N(6)-dimethyl-L-lysyl-[protein] + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:54196, Rhea:RHEA-COMP:13053, Rhea:RHEA-COMP:13827,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:61929, ChEBI:CHEBI:61976;
CC Evidence={ECO:0000250|UniProtKB:Q8N6R0};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=N-terminal glycyl-L-lysyl-L-glutamyl-[protein] + 3 S-adenosyl-
CC L-methionine = 3 H(+) + N-terminal N,N,N-trimethyl-glycyl-L-lysyl-L-
CC glutamyl-[protein] + 3 S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:58440, Rhea:RHEA-COMP:15140, Rhea:RHEA-COMP:15143,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:142597, ChEBI:CHEBI:142600;
CC Evidence={ECO:0000250|UniProtKB:Q8N6R0};
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC {ECO:0000305}.
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DR EMBL; AE014134; AAF53908.1; -; Genomic_DNA.
DR EMBL; AY061160; AAL28708.1; -; mRNA.
DR RefSeq; NP_610045.1; NM_136201.5.
DR AlphaFoldDB; Q9VIK9; -.
DR SMR; Q9VIK9; -.
DR BioGRID; 61290; 2.
DR STRING; 7227.FBpp0080918; -.
DR PaxDb; Q9VIK9; -.
DR DNASU; 35326; -.
DR EnsemblMetazoa; FBtr0081388; FBpp0080918; FBgn0032873.
DR GeneID; 35326; -.
DR KEGG; dme:Dmel_CG2614; -.
DR UCSC; CG2614-RA; d. melanogaster.
DR FlyBase; FBgn0032873; CG2614.
DR VEuPathDB; VectorBase:FBgn0032873; -.
DR eggNOG; KOG2352; Eukaryota.
DR GeneTree; ENSGT00510000047399; -.
DR InParanoid; Q9VIK9; -.
DR OrthoDB; 912165at2759; -.
DR PhylomeDB; Q9VIK9; -.
DR BioGRID-ORCS; 35326; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 35326; -.
DR PRO; PR:Q9VIK9; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0032873; Expressed in embryonic/larval hemocyte (Drosophila) and 24 other tissues.
DR ExpressionAtlas; Q9VIK9; baseline and differential.
DR Genevisible; Q9VIK9; DM.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.150; -; 2.
DR InterPro; IPR013216; Methyltransf_11.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR Pfam; PF08241; Methyltransf_11; 1.
DR SUPFAM; SSF53335; SSF53335; 2.
DR PROSITE; PS00867; CPSASE_2; 1.
PE 2: Evidence at transcript level;
KW Methyltransferase; Multifunctional enzyme; Reference proteome; Transferase.
FT CHAIN 1..673
FT /note="eEF1A lysine and N-terminal methyltransferase
FT homolog"
FT /id="PRO_0000310764"
SQ SEQUENCE 673 AA; 76003 MW; ECA8A9BFC384EB47 CRC64;
MNLLPKTREE FAQTDYWNEF FKKRGEKAFE WYGEYLELCD QIHKYIKPAD RILMLGCGNS
KLSMDMYDTG FRDITNIDIS PIAVKKMLEL NAKSRPEMKF LQMDATAMTF PDESFSVSLD
KGTLDALFAD DEPETRAVVE NYFKEILRTM RNGGRYVGIS LLQEHILNFL LDFLPKHNCM
LRIVHCLGVE QANKEKNADD ALTLPVFVVV ATKFKSLPMP VLEFGFGNDK MQRFTTVSEL
NSAVSSVQKA ALVCNGLARS NIAGHNEVIM DLHRPSEQTP RYTIHILDKP PARGLGKYAA
FIVPQGREVE WIFSTPAGRK KLQDSANFQR LAVVTLHRDQ VYSTLDEVKQ ELADSIKNLS
PAGLTDQIPY LSLGSDVGKR ETLICGFSKI SGDFRIEEVE ANGKTLRRLI FLSNQFVVQS
EALVKTVKIK GKKDRKKIDF GYLACQHHLY MSVGVQLATT VQHPKRDVEK DVLVVGLGGG
GLCSFLHAAL PQARITAVEI DPIMLEVAEQ YFELKQDKRF HVVIDDGLDF VERCRNEDIH
FDAVLFDVDS KDLSLGMSCP PQSFLATKIL QHIKEIIGPK GLFMLNLVCR DESLRTEALN
NLHKVFPAVC SYKLEEDINE IIYCANDEKY KTVEQWKKNM GTAGRGLNSA VKETKLASED
ALEVAEFLSE LKI
